Results 71 to 80 of about 392,164 (310)

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

In vitro blastocyst development of post-thaw vitrified bovine oocytes [PDF]

Veterinary World, 2013
Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro.Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15% ethylene glycol (EG) + 15 ...
D. J. Dutta,, Hiramoni Dev, Himangshu Raj   +2 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Candrian, Christian, Gianni-Barrera, Roberto, Juelke, Henriette, Martin, Ivan, Jaquiery, Claude, Acharya, Chitrangada, Barbero, Andrea, Miot, Sylvie, Pelttari, Karoliina, Mainil-Varlet, Pierre   +9 more
core   +1 more source

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Journal of Animal Science and Biotechnology, 2022
Background Heavy metal cadmium (Cd) is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and fertility.
Anna Rita Piras, Federica Ariu, Alessio Maltana, Giovanni Giuseppe Leoni, Nicola Antonio Martino, Antonella Mastrorocco, Maria Elena Dell’Aquila, Luisa Bogliolo   +7 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos [PDF]

, 2015
In vitro production (IVP) of bovine embryos is not only of great economic importance to the cattle industry, but is also an important model for studying embryo development.
Corrêa, Carolina A.P. [UNESP], Laura L.G. Lorena, João C.T. Penteado, Mingoti, Gisele, Penteado, João C.T., Lorena, Laura L.G., Arnold, Daniel R., Rossi, Guilherme F. [UNESP], Daniel R. Arnold, Guilherme F. Rossi, Flavia L. Lopes, Lopes, Flavia L. [UNESP], Rossi, Guilherme F., Ifran, Aderson M. [UNESP], Carolina A.P. Corrêa, Gaspar, Roberta C., Mingoti, Gisele [UNESP], Gisele Mingoti, Roberta C. Gaspar, Lorena, Laura L.G. [UNESP], Aderson M. Ifran, Corrêa, Carolina A.P., Ifran, Aderson M., Lopes, Flavia L., Penteado, João C.T. [UNESP], Gaspar, Roberta C. [UNESP]   +25 more
core   +1 more source

Altered cytoplasmic maturation in rescued in vitro matured oocytes

Human Reproduction, 2019
Do culture conditions affect cytoplasmic maturation in denuded immature non-GV oocytes?The maturation rate of denuded non-GV oocytes is not affected by culture media, but in vitro maturation seems to alter the mitochondrial membrane potential, endoplasmic reticulum (ER) and actin cytoskeleton compared with in vivo maturation.In vitro maturation of ...
A, Ferrer-Vaquer, M, Barragán, A, Rodríguez, R, Vassena   +3 more
openaire   +2 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source