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Advanced Science, EarlyView.ABSTRACT Brown and beige adipocytes dissipate energy as heat, yet effective strategies to enhance their mitochondrial efficiency remain limited. Here, we identify Agnuside (AGN) as a selective stabilizer of the complex I assembly factor NDUFAF6. AGN directly binds cytosolic NDUFAF6, suppresses its ubiquitination, prolongs its halfâlife, and facilitates
Qingwen Zhao +7 more
wiley +1 more source
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Rabbit blastocyst: Allocation of cells to the inner cell mass and trophectoderm
Molecular Reproduction and Development, 1995AbstractThe proportion of total cells in the blastocyst allocated to the inner cell mass (ICM) and trophectoderm (TE) is important for future development and may be a sensitive indicator to evaluate culture conditions. The number of cells and their distribution within the two primary cell lineages were determined for the rabbit embryo developing in ...
J R, Giles, R H, Foote
exaly +3 more sources
Nuclear transplantation by microinjection of inner cell mass and granulosa cell nuclei
Molecular Reproduction and Development, 1994AbstractThe developmental potential of bovine inner cell mass (ICM) and somatic differentiated (granulosa cell) nuclei was investigated using nuclear transplantation. ICM blastomeres were isolated after immunosurgery of day 7 in vitro produced blastocysts and cumulus granulosa cells recovered from in vitro matured oocytes.
P Collas
exaly +3 more sources
Mosaicism between trophectoderm and inner cell mass
Fertility and Sterility, 2017Defining the actual incidence and prevalence of mosaicism in human blastocysts still remains a difficult task. The small amount of evidence generated by animal and human studies does not support the existence of mechanisms involved in developmental arrest, clonal depletion, or aneuploidy rescue for abnormal cells in euploid/aneuploid embryos during ...
Antonio Capalbo, Laura Rienzi
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Differentiation of the inner cell mass of the baboon blastocyst
The Anatomical Record, 1990AbstractDuring the blastocyst stage of development in the baboon, the inner cell mass changes from an irregular accumulation of cells within the cavity of the blastocyst to a disk at one side of the blastocyst and finally to a spherial mass of epiblast cells exhibiting a distinct polarity.
A C, Enders, K C, Lantz, S, Schlafke
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Differentiation of Porcine Inner Cell Mass Cells Into Proliferating Neural Cells
Stem Cells and Development, 2010Here we report the culture and differentiation of porcine neural cells derived from the inner cell masses (ICMs) of blastocyst-stage embryos. Manually dissected ICMs were cultured on feeder layers of inactivated mouse embryonic fibroblasts (MEFs). Neural rosette-like structures were selected and passaged mechanically.
Puy, L. du +3 more
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Development, 1976
ABSTRACTInner cell masses (ICMs) dissected from -day rat blastocysts were aggregated with -day mouse morulae. Successful aggregates formed blastocysts in vitro and morphologically normal -day conceptuses in the mouse uterus. Immunofluorescent analysis of these conceptuses revealed that rat cells were only present in the embryonic ectoderm and endoderm ...
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ABSTRACTInner cell masses (ICMs) dissected from -day rat blastocysts were aggregated with -day mouse morulae. Successful aggregates formed blastocysts in vitro and morphologically normal -day conceptuses in the mouse uterus. Immunofluorescent analysis of these conceptuses revealed that rat cells were only present in the embryonic ectoderm and endoderm ...
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Microsurgery of Inner Cell Mass of Mouse Blastocysts
Nature, 1969MOUSE eggs can be immobilized for injection1,2 or for microsuction3, and rabbit blastocysts can be held to permit excision of trophoblast cells4. I describe here the use of a microsuction system3 to manipulate the inner cell mass of the mouse blastocyst.
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Control of inner cell mass development in cultured mouse blastocysts
Nature, 1977THE mouse blastocyst consists of a group of cells, the inner cell mass (ICM), attached to a small area of the inside surface of a sphere of trophectoderm cells. The trophecto-derm gives rise to the extraembryonic regions of the conceptus while the ICM forms the embryo proper1. At implantation trophoblast cells invade the uterine epithelium.
M, Monk, U, Petzoldt
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Production of mouse by inter-strain inner cell mass replacement
Zygote, 2005The developmental ability of reconstructed blastocysts from C57BL/6 strain mouse inner cell masses (ICMs) and Kunming strain mouse trophoblasts was assessed. The procedure of ICM replacement was as follows: C57BL/6 ICMs were separated from the blastocysts using immunosurgery.
Yue-Liang, Zheng +4 more
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