Insertion sequence transposition inactivates CRISPR-Cas immunity [PDF]
Nature Communications, 2023 CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified multiple natural transpositions of insertion sequences (ISs) into cas genes ...
Yong Sheng +9 more
doaj +4 more sources
Prevalence of insertion sequence elements in plasmids relating to mgrB gene disruption causing colistin resistance in Klebsiella pneumoniae [PDF]
MicrobiologyOpen, 2022 Colistin is a last resort antibiotic for the treatment of carbapenemase producing Klebsiella pneumoniae. The disruption of the mgrB gene by insertion sequences (ISs) is a mechanism mediating colistin resistance.
Stephen Mark Edward Fordham +2 more
doaj +3 more sources
No Isolate, No Problem: Using a Novel Insertion Sequence PCR to Link Rats to Human Shigellosis Cases in an Underserved Urban Community [PDF]
Microbiology Spectrum, 2023 During an investigation into a cluster of Shigella flexneri serotype 2a cases in an underserved community, we assessed the relatedness of human and rat S.
Gordon Ritchie +9 more
doaj +2 more sources
Scarless excision of an insertion sequence in the OmpK36 promoter restores meropenem susceptibility in a non-carbapenemase-producing Klebsiella pneumoniae [PDF]
Emerging Microbes and InfectionsCarbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant global health challenge due to its limited treatment options and high mortality rates.
Yingying Du +7 more
doaj +2 more sources
Insertion sequence transposition activates antimycobacteriophage immunity through an lsr2‐silenced lipid metabolism gene island [PDF]
mLifeInsertion sequences (ISs) exist widely in bacterial genomes, but their roles in the evolution of bacterial antiphage defense remain to be clarified. Here, we report that, under the pressure of phage infection, the IS1096 transposition of Mycobacterium ...
Yakun Li +6 more
doaj +2 more sources
The early phase of a bacterial insertion sequence infection [PDF]
Theoretical Population Biology, 2010 Bacterial insertion sequences are the simplest form of autonomous mobile DNA. It is unknown whether they need to have beneficial effects to infect and persist in bacterial populations, or whether horizontal gene transfer suffices for their persistence ...
Andreas Wagner
exaly +2 more sources
Estimating the fitness effect of an insertion sequence [PDF]
Journal of Mathematical Biology, 2012 Since its discovery, mobile DNA has fascinated researchers. In particular, many researchers have debated why insertion sequences persist in prokaryote genomes and populations. While some authors think that insertion sequences persist only because of occasional beneficial effects they have on their hosts, others argue that horizontal gene transfer is ...
A D Barbour, Andreas Wagner
exaly +4 more sources
Templated sequence insertion polymorphisms in the human genome [PDF]
Frontiers in Chemistry, 2016 Templated Sequence Insertion Polymorphism (TSIP) is a recently described form of polymorphism recognized in the human genome, in which a sequence that is templated from a distant genomic region is inserted into the genome, seemingly at random.
Masahiro Onozawa +2 more
doaj +3 more sources
Insertion sequences disrupting mgrB in carbapenem-resistant Klebsiella pneumoniae strains in Brazil
Journal of Global Antimicrobial Resistance, 2021 Objectives: This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp). Methods: A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (
Daniely Martins da Silva +7 more
doaj +1 more source
Microbiology and Molecular Biology Reviews, 1998 SUMMARY Insertion sequences (ISs) constitute an important component of most bacterial genomes. Over 500 individual ISs have been described in the literature to date, and many more are being discovered in the ongoing prokaryotic and eukaryotic genome-sequencing projects. The last 10 years have also seen some striking advances in
Mahillon, Jacques, Chandler, M.
openaire +3 more sources