Targeted DNA insertion in plants
Conventional methods of DNA sequence insertion into plants, using Agrobacterium-mediated transformation or microprojectile bombardment, result in the integration of the DNA at random sites in the genome.
O. Dong, P. Ronald
semanticscholar +1 more source
Site-specific transposition of insertion sequence IS630 [PDF]
IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene.
T, Tenzen, S, Matsutani, E, Ohtsubo
openaire +2 more sources
Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis [PDF]
The entomopathogenic nematode Heterorhabditis bacteriophora forms a specific mutualistic association with its bacterial partner Photorhabdus temperata.
Abebe-Akele, Feseha +9 more
core +2 more sources
ISMapper: identifying transposase insertion sites in bacterial genomes from short read sequence data
Insertion sequences (IS) are small transposable elements, commonly found in bacterial genomes. Identifying the location of IS in bacterial genomes can be useful for a variety of purposes including epidemiological tracking and predicting antibiotic ...
J. Hawkey +6 more
semanticscholar +1 more source
Import of honeybee prepromelittin into the endoplasmic reticulum [PDF]
Honeybee prepromelittin is correctly processed and imported by dog pancreas microsomes. Insertion of prepromelittin into microsomal membranes, as assayed by signal sequence removal, does not depend on signal recognition particle (SRP) and docking protein.
Müller, Günter, Zimmermann, Richard
core +2 more sources
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Genome-wide analysis of the emigrant family of MITEs: amplification dynamics and evolution of genes in Arabidopsis thaliana [PDF]
MITEs are structurally similar to defective class II elements but their high copy number and the size and sequence conservation of most MITE families suggest that they can be amplified by a replicative mechanism. Here we present a genome-wide analysis
Casacuberta, Josep Maria +4 more
core +1 more source
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang +5 more
wiley +1 more source
The nucleotide sequence of a human immnnoglobulin C-gamma-1 gene [PDF]
We report the nucleotide sequence of a gene encoding the constant region of a human immnnoglobulin γ1 heavy chain (Cγ1). A comparison of this sequence with those of the Cγ2 and Cγ4 genes reveals that these three human Cγ genes share considerable homology
Berson, Bennett J. +2 more
core
Targeting of the master receptor MOM19 to mitochondria [PDF]
The targeting of proteins to mitochondria involves the recognition of the precursor proteins by receptors on the mitochondrial surface followed by insertion of the precursors into the outer membrane at the general insertion site GIP.
Dietmeier, Klaus A. +7 more
core +1 more source

