Results 11 to 20 of about 199,615 (291)

VisioProt-MS: interactive 2D maps from intact protein mass spectrometry. [PDF]

Bioinformatics, 2019
Abstract Summary VisioProt-MS is designed to summarize and analyze intact protein and top-down proteomics data. It plots the molecular weights of eluting proteins as a function of their retention time, thereby allowing inspection of runs from liquid chromatography coupled to mass spectrometry (LC-MS).
Locard-Paulet M, Parra J, Albigot R, Mouton-Barbosa E, Bardi L, Burlet-Schiltz O, Marcoux J.   +6 more
europepmc   +6 more sources

Mass spectrometry of intact membrane protein complexes [PDF]

Nature Protocols, 2013
Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful technique to study the stoichiometry, structure-function and dynamics of protein assemblies. Recent developments have extended this technique to the study of membrane protein complexes, where it has already revealed subunit stoichiometries and specific phospholipid ...
Laganowsky, A, Reading, E, Hopper, JT, Robinson, CV   +3 more
openaire   +4 more sources

Investigation of intact protein complexes by mass spectrometry [PDF]

Mass Spectrometry Reviews, 2004
Abstract    I. Introduction 00   II. Electrospray Ionization of Biomacromolecules 00   III. Mass Analyzers for Biomacromolecular Mass Spectrometry 00 A.  Collisional Cooling and/or Focusing 00 B.  Alternative Mass Analyzers 00  IV. Solution‐Phase Properties of Proteins Complexes 00 A.
Heck, A.J.R., van den Heuvel, R.H.H.
openaire   +3 more sources

Mass Spectrometry Analysis of Intact Proteins from Crude Samples [PDF]

Analytical Chemistry, 2020
Analysis of intact proteins by native mass spectrometry has emerged as a powerful tool for obtaining insight into subunit diversity, post-translational modifications, stoichiometry, structural arrangement, stability, and overall architecture. Typically, such an analysis is performed following protein purification procedures, which are time consuming ...
Shay Vimer, Gili Ben-Nissan, Michal Sharon   +2 more
openaire   +2 more sources

Analysis of Intact Protein Isoforms by Mass Spectrometry [PDF]

Journal of Biological Chemistry, 2011
The diverse proteome of an organism arises from such events as single nucleotide substitutions at the DNA level, different RNA processing, and dynamic enzymatic post-translational modifications. This minireview focuses on the measurement of intact proteins to describe the diversity found in proteomes.
Jeremiah D, Tipton, John C, Tran, Adam D, Catherman, Dorothy R, Ahlf, Kenneth R, Durbin, Neil L, Kelleher   +5 more
openaire   +2 more sources

Electrospray‐ionization mass spectrometry of intact intrinsic membrane proteins [PDF]

Protein Science, 1998
AbstractMembrane proteins drive and mediate many essential cellular processes making them a vital section of the proteome. However, the amphipathic nature of these molecules ensures their detailed structural analysis remains challenging. A versatile procedure for effective electrospray‐ionization mass spectrometry (ESI‐MS) of intact intrinsic membrane ...
J P, Whitelegge, C B, Gundersen, K F, Faull   +2 more
openaire   +2 more sources

Sequencing intact membrane proteins using MALDI mass spectrometry

Frontiers in Analytical Science, 2023
Membrane proteins are key players in many cellular events and represent crucial drug targets. Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) is a valuable approach to investigate them. To our knowledge, there are only a few reports of sequencing small membrane proteins using MALDI in-source decay (ISD).
Zhamungui Sánchez, Edison, Hijazi, Hassan, Haidar, Jana, Mecarelli, Enrica, Bauda, Elda, Petit-Härtlein, Isabelle, Teulon, Jean-Marie, Pellequer, Jean-Luc, Boeri Erba, Elisabetta   +8 more
openaire   +3 more sources

Mass graphs and their applications in top-down proteomics [PDF]

, 2015
Although proteomics has made rapid progress in the past decade, researchers are still in the early stage of exploring the world of complex proteoforms, which are protein products with various primary structure alterations resulting from gene mutations ...
Kou, Qiang, Liu, Xiaowen, Pasa-Tolić, Ljiljana, Tolić, Nikola, Wu, Si   +4 more
core   +2 more sources

Charge Reduction Stabilizes Intact Membrane Protein Complexes for Mass Spectrometry [PDF]

Journal of the American Chemical Society, 2014
The study of intact soluble protein assemblies by means of mass spectrometry is providing invaluable contributions to structural biology and biochemistry. A recent breakthrough has enabled similar study of membrane protein complexes, following their release from detergent micelles in the gas phase.
Mehmood, Shahid, Marcoux, Julien, Hopper, Jonathan, Allison, Timothy, Liko, Idlir, Borysik, Antoni, Robinson, Carol   +6 more
openaire   +3 more sources

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors [PDF]

, 2020
Detergents enable the purification of membrane proteins and are indispensable reagents instructural biology. Even though a large variety of detergents have been developed in the lastcentury, the challenge remains to identify guidelines that allowfine ...
Almeida, F., Bolla, J., Ehrmann, S., Gault, J., Haag, R., Hoi, K., Liko, I., Pagel, K., Robinson, C., Schweder, M., Shutin, D., Urner, L., Yen, H.   +12 more
core   +2 more sources