iScience, 2021 Summary: Standard methods for mass analysis measure ensembles of thousand to millions of molecules. This approach enables analysis of monodisperse recombinant proteins, whereas some heterogeneous protein assemblies pose a significant challenge, whereby ...
Szu-Hsueh Lai +2 more
doaj +1 more source
Limited proteolysis in the investigation of beta2-microglobulin amyloidogenic and fibrillar states. [PDF]
, 2005 Amyloid fibrils of patients treated with regular haemodialysis essentially consists of β2-microglobulin (β2-m) and its truncated species ΔN6β2-m lacking six residues at the amino terminus.
AMORESANO, ANGELA +4 more
core +1 more source
Integrating mass spectrometry of intact protein complexes into structural proteomics [PDF]
PROTEOMICS, 2012 MS analysis of intact protein complexes has emerged as an established technology for assessing the composition and connectivity within dynamic, heterogeneous multiprotein complexes at low concentrations and in the context of mixtures. As this technology continues to move forward, one of the main challenges is to integrate the information content of ...
Hyung, Suk‐joon, Ruotolo, Brandon T.
openaire +3 more sources
Top-Down Mass Analysis of Protein Tyrosine Nitration: Comparison of Electron Capture Dissociation with “Slow-Heating” Tandem Mass Spectrometry Methods [PDF]
, 2010 Tyrosine nitration in proteins is an important post-translational modification (PTM) linked to various pathological conditions. When multiple potential sites of nitration exist, tandem mass spectrometry (MS/MS) methods provide unique tools to locate the ...
Cooper, Helen J. +2 more
core +2 more sources
Mass spectrometry of intact protein complexes [PDF]
Nature Methods, 2013 Mass spectrometry technology to detect and characterize large, intact protein complexes is becoming more accessible.
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Protein complexes in cells by AI‐assisted structural proteomics
Molecular Systems Biology, 2023 Accurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable a candidate‐based approach to systematically model novel protein assemblies.
Francis J O'Reilly +9 more
doaj +1 more source
Mass Spectrometry in the Elucidation of the Glycoproteome of Bacterial Pathogens [PDF]
, 2010 Presently some three hundred post-translational modifications are known to occur in bacteria in vivo. Many of these modifications play critical roles in the regulation of proteins and control key biological processes.
Graham, Robert L. J., Hess, Sonja
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Structural Plasticity and Noncovalent Substrate Binding in the GroEL Apical Domain. A study using electrospray ionization mass spectrometry and fluorescence binding studies [PDF]
, 2002 Advances in understanding how GroEL binds to non-native proteins are reported. Conformational flexibility in the GroEL apical domain, which could account for the variety of substrates that GroEL binds, is illustrated by comparison of several independent ...
Adams +62 more
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Shotgun ion mobility mass spectrometry sequencing of heparan sulfate saccharides [PDF]
, 2020 Despite evident regulatory roles of heparan sulfate (HS) saccharides in numerous biological processes, definitive information on the bioactive sequences of these polymers is lacking, with only a handful of natural structures sequenced to date.
Grabarics, Márkó +9 more
core +1 more source
Ambient surface mass spectrometry–ion mobility spectrometry of intact proteins
Current Opinion in Chemical Biology, 2018 Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application.
Rian L, Griffiths +2 more
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