Results 1 to 10 of about 31,694 (314)

New Applications for Phage Integrases [PDF]

Journal of Molecular Biology, 2014
Within the last twenty-five years bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering.
Abremski, Albert, Andreas, Astumian, Atlung, Auvray, Bai, Ball, Baltz, Baltz, Bateman, Baumgardner, Becher, Bibb, Biswas, Bonnet, Bonnet, Branda, Breüner, Brown, Campbell, Campbell, Chalberg, Chavez, Cho, Colloms, Craig, Dafhnis-Calas, Dhar, Esposito, Freuler, Friedland, Ghosh, Ghosh, Ghosh, Giuraniuc, Goodman, Gregory, Grindley, Groth, Groth, Hakimi, Ham, Hambly, Hartley, Hauser, Hauser, Hazelbaker, Hoang, Hoess, Hu, Irion, Kapusi, Kempe, Khaleel, Kuhstoss, Kuhstoss, Langer, Lee, Lee, Leong, Lewis, Lewis, Li, Lister, LoVullo, Malla, Mandali, Margaret C.M. Smith, Marshall Stark, Martin, Mattis, Meredith, Miyazaki, Mizuuchi, Monetti, Morita, Nimmo, Olivares, Olivares, Olorunniji, Ortiz-Urda, Ortiz-Urda, Paul C.M. Fogg, Pedulla, Peña, Peña, Ptashne, Rashel, Ravatn, Raymond, Riggs, Rodriguez, Ross, Rutherford, Sauer, Savinov, Schlake, Sean Colloms, Siuti, Smith, Smith, Smith, Smith, Smith-Mungo, Smokvina, Soriano, St-Pierre, Stark, Stoll, Stover, Susan Rosser, Suttle, Sylwan, Tasic, Thomson, Thorpe, Thorpe, Thyagarajan, Turan, Van Duyne, Van Duyne, Venken, Venken, Xu, Xu, Xu, Ye, Yin, Yonemura, Yonemura, Yu, Yu, Yu, Yu, Zhang, Zhang, Zhang   +137 more
core   +9 more sources

Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases [PDF]

Biomolecules, 2021
Integrases of different retroviruses assemble as functional complexes with varying multimers of the protein. Retroviral integrases require a divalent metal cation to perform one-step transesterification catalysis.
Anthony J. Rabe, Yow Yong Tan, Ross C. Larue, Kristine E. Yoder   +3 more
doaj   +4 more sources

Integrase and integration: biochemical activities of HIV-1 integrase [PDF]

Retrovirology, 2008
Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration.
Deprez Eric, Saïb Ali, Carayon Kevin, Delelis Olivier, Mouscadet Jean-François   +4 more
doaj   +4 more sources

A population‐based temporal logic gate for timing and recording chemical events [PDF]

Molecular Systems Biology, 2016
Engineered bacterial sensors have potential applications in human health monitoring, environmental chemical detection, and materials biosynthesis. While such bacterial devices have long been engineered to differentiate between combinations of inputs ...
Victoria Hsiao, Yutaka Hori, Paul WK Rothemund, Richard M Murray   +3 more
doaj   +3 more sources

Application of serine integrases for secondary metabolite pathway assembly in Streptomyces [PDF]

Synthetic and Systems Biotechnology, 2020
Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be ...
Hong Gao, Gabrielle Taylor, Stephanie K. Evans, Paul C.M. Fogg, Margaret C.M. Smith   +4 more
doaj   +4 more sources

Genetic characteristics and integration specificity of Salmonella enterica temperate phages [PDF]

Frontiers in Microbiology, 2023
IntroductionTemperate phages can engage in the horizontal transfer of functional genes to their bacterial hosts. Thus, their genetic material becomes an intimate part of bacterial genomes and plays essential roles in bacterial mutation and evolution ...
Siqi Sun, Siqi Sun, Xianglilan Zhang
doaj   +2 more sources

Substrate specificity of Ty1 integrase [PDF]

Journal of Virology, 1995
Integration of the Saccharomyces cerevisiae retrotransposon Ty1 requires the element-encoded integrase (IN) protein, which is a component of cytoplasmic virus-like particles (VLPs). Using purified recombinant Ty1 IN and an oligonucleotide integration assay based on Ty1 long terminal repeat sequences, we have compared IN activity on substrates having ...
S P Moore, Maureen A. Powers, David Garfinkel   +2 more
openalex   +3 more sources

Variable orthogonality of serine integrase interactions within the ϕC31 family [PDF]

Scientific Reports
Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage ...
Alasdair I. MacDonald, Aron Baksh, Alexandria Holland, Heewhan Shin, Phoebe A. Rice, W. Marshall Stark, Femi J. Olorunniji   +6 more
doaj   +2 more sources

There and turn back again: the application of phage serine integrases in eukaryotic systems [PDF]

Frontiers in Bioengineering and Biotechnology
Serine integrases (Ints) have gained prominence and have been extensively used in Synthetic Biology due to their ability to modify DNA sequences. Ints are recombinases encoded by the phage genome and have been used to unidirectionally catalyze an ...
Thais Torquato Sales, Thais Torquato Sales, Marco Antônio de Oliveira, Marco Antônio de Oliveira, Lilian Hasegawa Florentino, Lilian Hasegawa Florentino, Rayane Nunes Lima, Elibio Rech   +7 more
doaj   +2 more sources

Prevalence of SOS-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons [PDF]

Mobile DNA, 2011
Background Integrons are found in hundreds of environmental bacterial species, but are mainly known as the agents responsible for the capture and spread of antibiotic-resistance determinants between Gram-negative pathogens.
Cambray Guillaume, Sanchez-Alberola Neus, Campoy Susana, Guerin Émilie, Da Re Sandra, González-Zorn Bruno, Ploy Marie-Cécile, Barbé Jordi, Mazel Didier, Erill Ivan   +9 more
doaj   +12 more sources