Results 11 to 20 of about 13,949 (234)
Integrase and integration: biochemical activities of HIV-1 integrase [PDF]
Retrovirology, 2008 Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration.
Deprez Eric +4 more
doaj +4 more sources
Substrate specificity of Ty1 integrase [PDF]
Journal of Virology, 1995 Integration of the Saccharomyces cerevisiae retrotransposon Ty1 requires the element-encoded integrase (IN) protein, which is a component of cytoplasmic virus-like particles (VLPs). Using purified recombinant Ty1 IN and an oligonucleotide integration assay based on Ty1 long terminal repeat sequences, we have compared IN activity on substrates having ...
S P Moore +2 more
openalex +3 more sources
Angewandte Chemie, Volume 134, Issue 51, December 19, 2022., 2022 Benzoxazolinate is a rare bis‐heterocyclic moiety that interacts with proteins and DNA. It was found that a putative acyl AMP‐ligase mediates the last cyclization step to afford benzoxazolinate. The enzyme was then used as a probe for genome mining, which led to the discovery of a new class of benzoxazolinate‐containing compounds in diverse bacteria ...
Yi‐Ming Shi +12 more
wiley +2 more sources
Structure and function of retroviral integrase [PDF]
Nature Reviews Microbiology, 2021 A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the viral enzyme responsible for the catalytic steps involved in this process, and integrase strand transfer inhibitors are widely used to treat people living with HIV ...
Alan Engelman +3 more
openaire +4 more sources
Retroviral integrases and their cousins [PDF]
Current Opinion in Structural Biology, 1996 The recently determined structures of the catalytic domains of HIV integrase, avian sarcoma virus integrase and the Mu transposase are strikingly similar to each other and also exhibit significant similarity to several nucleases. All these enzymes of cut polynucleotides, leaving 3'OH and 5'PO4 groups. The integrase and transposase also possess a strand-
Rice, Phoebe +2 more
openaire +3 more sources
Cancer Communications, Volume 42, Issue 12, Page 1257-1287, December 2022., 2022 Abstract Clustered regularly interspaced short palindromic repeats‐associated protein (CRISPR/Cas9), an adaptive microbial immune system, has been exploited as a robust, accurate, efficient and programmable method for genome targeting and editing. This innovative and revolutionary technique can play a significant role in animal modeling, in vivo genome
Khaled S. Allemailem +6 more
wiley +1 more source
Resistance to Integrase Inhibitors [PDF]
Viruses, 2010 Integrase (IN) is a clinically validated target for the treatment of human immunodeficiency virus infections and raltegravir exhibits remarkable clinical activity. The next most advanced IN inhibitor is elvitegravir. However, mutant viruses lead to treatment failure and mutations within the IN coding sequence appear to confer cross-resistance.
Yves Pommier +3 more
openaire +4 more sources
Synthesis of programmable integrases [PDF]
Proceedings of the National Academy of Sciences, 2009 Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes ...
Charles A. Gersbach +2 more
openaire +3 more sources
Retroviral Integrase: Then and Now [PDF]
Annual Review of Virology, 2015 The retroviral integrases are virally encoded, specialized recombinases that catalyze the insertion of viral DNA into the host cell's DNA, a process that is essential for virus propagation. We have learned a great deal since the existence of an integrated form of retroviral DNA (the provirus) was first proposed by Howard Temin in 1964. Initial studies
Anna Marie Skalka, Mark Andrake
openaire +3 more sources
A little less aggregation a little more replication: Viral manipulation of stress granules
WIREs RNA, Volume 14, Issue 1, January/February 2023., 2023 Viruses depend entirely on host machinery and therefore aim to conquer the host and defeat its defenses to co‐opt its resources for its own replication. Improved understanding of how viruses counteract host immune responses such as stress granule assembly, detailed here, will inform future antiviral therapeutic strategies.
Matthew J. Brownsword, Nicolas Locker
wiley +1 more source