Results 21 to 30 of about 14,447 (191)

Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Nature Communications, 2019
Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ...
Marcus D. Wilson, Ludovic Renault, Daniel P. Maskell, Mohamed Ghoneim, Valerie E. Pye, Andrea Nans, David S. Rueda, Peter Cherepanov, Alessandro Costa   +8 more
doaj   +1 more source

Influence of the amino-terminal sequence on the structure and function of HIV integrase

Retrovirology, 2020
Background Antiretroviral therapy (ART) can mitigate the morbidity and mortality caused by the human immunodeficiency virus (HIV). Successful development of ART can be accelerated by accurate structural and biochemical data on targets and their responses
Grant Eilers, Kushol Gupta, Audrey Allen, Jeffrey Zhou, Young Hwang, Michael B. Cory, Frederic D. Bushman, Gregory Van Duyne   +7 more
doaj   +1 more source

A snapshot on HIV-1 evolution through the identification of phylogenetic-specific properties of HIV-1 integrases M/O

PLoS Pathogens, 2023
Transmissions of simian viruses to humans has originated the different groups of HIV-1. We recently identified a functional motif (CLA), in the C-terminal domain of the integrase, essential for integration in HIV-1 group M.
Elenia Toccafondi, Marine Kanja, Flore Winter, Daniela Lener, Matteo Negroni   +4 more
doaj   +2 more sources

Integrase and integration: biochemical activities of HIV-1 integrase

Retrovirology, 2008
Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration.
Deprez Eric, Saïb Ali, Carayon Kevin, Delelis Olivier, Mouscadet Jean-François   +4 more
doaj   +1 more source

Metagenomic community composition and resistome analysis in a full-scale cold climate wastewater treatment plant

Environmental Microbiome, 2022
Background Wastewater treatment plants are an essential part of maintaining the health and safety of the general public. However, they are also an anthropogenic source of antibiotic resistance genes.
Paul Jankowski, Jaydon Gan, Tri Le, Michaela McKennitt, Audrey Garcia, Kadir Yanaç, Qiuyan Yuan, Miguel Uyaguari-Diaz   +7 more
doaj   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Rational Design of Optical Single‐Walled Carbon Nanotube‐Based Nanosensors with Biological Recognition Elements

Advanced Sensor Research, EarlyView.
This Review focuses on assessing and providing perspective on the field of rationally‐designed optical sensors constructed with single‐walled carbon nanotubes. The literature is reviewed and evaluated for SWCNT‐based sensors constructed with biomolecular recognition elements, including proteins, peptides, and oligonucleotides, as well as their methods ...
Amelia K. Ryan, Atara Israel, Maria Celina Stefoni, Catarina Ferraz, Ryan M. Williams   +4 more
wiley   +1 more source

Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation

Nature Communications, 2016
The integron integrases have evolved to perform recombination of single and double stranded DNA. Here the authors show that the ancestral pathway is still functional at double stranded sites, revealing the evolution towards the modern resolution pathway.
Jose Antonio Escudero, Celine Loot, Vincent Parissi, Aleksandra Nivina, Christiane Bouchier, Didier Mazel   +5 more
doaj   +1 more source

Effect of attC structure on cassette excision by integron integrases

Mobile DNA, 2011
Background Integrons are genetic elements able to integrate and disseminate genes as cassettes by a site-specific recombination mechanism. These elements contain a gene coding for an integrase that carries out recombination by interacting with two ...
Larouche André, Roy Paul H
doaj   +1 more source

Genomic and Functional Characterization of Vancomycin-Resistant Enterococci-Specific Bacteriophages in the Galleria mellonella Wax Moth Larvae Model

Pharmaceutics, 2022
Phages are naturally occurring viruses that selectively kill bacterial species without disturbing the individual’s normal flora, averting the collateral damage of antimicrobial usage.
Lynn El Haddad, Georgios Angelidakis, Justin R. Clark, Jesus F. Mendoza, Austen L. Terwilliger, Christopher P. Chaftari, Mark Duna, Serena T. Yusuf, Cynthia P. Harb, Mark Stibich, Anthony Maresso, Roy F. Chemaly   +11 more
doaj   +1 more source