Results 21 to 30 of about 9,111 (181)

Synthesis of programmable integrases [PDF]

Proceedings of the National Academy of Sciences, 2009
Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes ...
Russell M, Gordley, Charles A, Gersbach, Carlos F, Barbas   +2 more
openaire   +2 more sources

Resistance to Integrase Inhibitors [PDF]

Viruses, 2010
Integrase (IN) is a clinically validated target for the treatment of human immunodeficiency virus infections and raltegravir exhibits remarkable clinical activity. The next most advanced IN inhibitor is elvitegravir. However, mutant viruses lead to treatment failure and mutations within the IN coding sequence appear to confer cross-resistance.
Mathieu Métifiot, Christophe Marchand, Kasthuraiah Maddali, Yves Pommier   +3 more
openaire   +3 more sources

Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

BMC Biotechnology, 2019
Background Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms.
Hong Gao, Buvani Murugesan, Janina Hoßbach, Stephanie K. Evans, W. Marshall Stark, Margaret C. M. Smith   +5 more
doaj   +1 more source

Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Nature Communications, 2019
Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ...
Marcus D. Wilson, Ludovic Renault, Daniel P. Maskell, Mohamed Ghoneim, Valerie E. Pye, Andrea Nans, David S. Rueda, Peter Cherepanov, Alessandro Costa   +8 more
doaj   +1 more source

Influence of the amino-terminal sequence on the structure and function of HIV integrase

Retrovirology, 2020
Background Antiretroviral therapy (ART) can mitigate the morbidity and mortality caused by the human immunodeficiency virus (HIV). Successful development of ART can be accelerated by accurate structural and biochemical data on targets and their responses
Grant Eilers, Kushol Gupta, Audrey Allen, Jeffrey Zhou, Young Hwang, Michael B. Cory, Frederic D. Bushman, Gregory Van Duyne   +7 more
doaj   +1 more source

New Applications for Phage Integrases [PDF]

Journal of Molecular Biology, 2014
Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular ...
Fogg, Paul C M, Colloms, Sean, Rosser, Susan, Stark, Marshall, Smith, Margaret C M   +4 more
openaire   +5 more sources

A snapshot on HIV-1 evolution through the identification of phylogenetic-specific properties of HIV-1 integrases M/O

PLoS Pathogens, 2023
Transmissions of simian viruses to humans has originated the different groups of HIV-1. We recently identified a functional motif (CLA), in the C-terminal domain of the integrase, essential for integration in HIV-1 group M.
Elenia Toccafondi, Marine Kanja, Flore Winter, Daniela Lener, Matteo Negroni   +4 more
doaj   +2 more sources

Metagenomic community composition and resistome analysis in a full-scale cold climate wastewater treatment plant

Environmental Microbiome, 2022
Background Wastewater treatment plants are an essential part of maintaining the health and safety of the general public. However, they are also an anthropogenic source of antibiotic resistance genes.
Paul Jankowski, Jaydon Gan, Tri Le, Michaela McKennitt, Audrey Garcia, Kadir Yanaç, Qiuyan Yuan, Miguel Uyaguari-Diaz   +7 more
doaj   +1 more source

Genomic and Functional Characterization of Vancomycin-Resistant Enterococci-Specific Bacteriophages in the Galleria mellonella Wax Moth Larvae Model

Pharmaceutics, 2022
Phages are naturally occurring viruses that selectively kill bacterial species without disturbing the individual’s normal flora, averting the collateral damage of antimicrobial usage.
Lynn El Haddad, Georgios Angelidakis, Justin R. Clark, Jesus F. Mendoza, Austen L. Terwilliger, Christopher P. Chaftari, Mark Duna, Serena T. Yusuf, Cynthia P. Harb, Mark Stibich, Anthony Maresso, Roy F. Chemaly   +11 more
doaj   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source