Results 21 to 30 of about 31,694 (314)
Advances in Biomedical and Health Sciences, 2022 Antibiotic resistance genes (ARGs) are classified as emerging environmental pollutants of global public health concern. These ARGs are disseminated through genetic elements such as integrons.
Anthony Ifeanyin Okoh +1 more
doaj +1 more source
Structure and function of retroviral integrase [PDF]
Nature Reviews Microbiology, 2021 A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the viral enzyme responsible for the catalytic steps involved in this process, and integrase strand transfer inhibitors are widely used to treat people living with HIV ...
Alan Engelman +3 more
openaire +4 more sources
Applications of Serine Integrases in Synthetic Biology over the Past Decade
SynBio, 2023 Serine integrases are emerging as one of the most powerful biological tools for biotechnology. Over the past decade, many research papers have been published on the use of serine integrases in synthetic biology.
Fang Ba +4 more
semanticscholar +1 more source
Systematic Discovery of a New Catalogue of Tyrosine-Type Integrases in Bacterial Genomic Islands
Applied and Environmental Microbiology, 2023 Site-specific recombinases or integrases have high specificity for DNA large fragment integration, which is urgently needed for gene editing. However, known integrases are not sufficient for meeting multiple integrations.
Gang Zhang +7 more
semanticscholar +1 more source
High fidelity one‐pot DNA assembly using orthogonal serine integrases
Biotechnology Journal, 2022 Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is limited by
J. Abioye +7 more
semanticscholar +1 more source
Retroviral integrases and their cousins [PDF]
Current Opinion in Structural Biology, 1996 The recently determined structures of the catalytic domains of HIV integrase, avian sarcoma virus integrase and the Mu transposase are strikingly similar to each other and also exhibit significant similarity to several nucleases. All these enzymes of cut polynucleotides, leaving 3'OH and 5'PO4 groups. The integrase and transposase also possess a strand-
Rice, Phoebe +2 more
openaire +3 more sources
Resistance to Integrase Inhibitors [PDF]
Viruses, 2010 Integrase (IN) is a clinically validated target for the treatment of human immunodeficiency virus infections and raltegravir exhibits remarkable clinical activity. The next most advanced IN inhibitor is elvitegravir. However, mutant viruses lead to treatment failure and mutations within the IN coding sequence appear to confer cross-resistance.
Yves Pommier +3 more
openaire +4 more sources
A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
doaj +1 more source
Access Microbiology, 2021 Some major producers of useful bioactive natural products belong to the genus Streptomyces or related actinobacteria. Genetic engineering of these bacteria and the pathways that synthesize their valuable products often relies on serine integrases.
Hong Gao, Margaret C. M. Smith
semanticscholar +1 more source
Retroviral Integrase: Then and Now [PDF]
Annual Review of Virology, 2015 The retroviral integrases are virally encoded, specialized recombinases that catalyze the insertion of viral DNA into the host cell's DNA, a process that is essential for virus propagation. We have learned a great deal since the existence of an integrated form of retroviral DNA (the provirus) was first proposed by Howard Temin in 1964. Initial studies
Anna Marie Skalka, Mark Andrake
openaire +3 more sources