Results 291 to 300 of about 199,768 (345)

Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein

Nature, 1990
A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of ...
Donald B. Carter, Scott E. Truesdell, Daniel E. Tracey, H. A. Zurcher-Neely, Robert N. McEwan, M. M. Hardee, Michael J. Bienkowski, J G Chosay, Frank F. Sun, J. A. Shelly, Colin J. Dunn, Alice L. Laborde, G. A. Waszak, Robert L. Heinrikson, I M Reardon, Ann E. Berger, Thomas E. Eessalu, Anthony W. Yem, Che-Shen C. Tomich, B. M. Taylor, L. C. Sieu, Jerry L. Slightom, Peter K. W. Harris, Martin R. Deibel   +23 more
openaire   +4 more sources

Interleukin-1 Receptor Antagonist Protein and mRNA in the Rat Adrenal Gland

Journal of Interferon & Cytokine Research, 1995
The occurrence of the endogenous receptor antagonist for the cytokine interleukin-1 in the rat adrenal gland was analyzed y polymerase chain reaction and by immunohistochemistry using a rabbit polyclonal antiserum. Expression of interleukin-1 receptor antagonist mRNA was demonstrated in both adrenal medulla and cortex, and a marked increase in the ...
Johan Lundkvist, Stefan B. Svenson, Tamas Bartfai, Stefan Nobel, András Simoncsits, Susanne Tingsborg, Marianne Schultzberg   +6 more
openaire   +4 more sources

Successful treatment of Erdheim-Chester disease by interleukin-1 receptor antagonist protein

Joint Bone Spine, 2014
Erdheim-Chester disease is a rare non-langerhans systemic histiocytosis of unknown origin, associated with bone diseases and severe visceral complications. Therapies have been disappointing. A recombinant form of interleukin-1 receptor antagonist (anakinra) has been used in a few cases when usual treatment fails.
E. Vignot, Roland Chapurlat, Audrey Courcoul   +2 more
openaire   +4 more sources

Beneficial Effect of Interleukin-1 Receptor Antagonist Protein on Spinal Cord Injury Recovery in the Rat [PDF]

Inflammation, 2011
We assessed the effect of treatment with the interleukin-1 receptor antagonist protein (IRAP) on morphological and functional recovery in a rat model of SCI. All sections were processed for immunohistochemistry, hematoxylin-eosin, and Nissl staining. Rats were assessed for hind limb motor function using the Basso, Beattie, and Bresnahan (BBB) hind limb
Shaohui Zong, Yuxi Zhao, Bo Wei, Chunxiang Xiong, Gaofeng Zeng   +4 more
openaire   +3 more sources

Cytokine regulation of the interleukin‐1 receptor antagonist protein in U937 cells

European Journal of Immunology, 1993
AbstractA naturally occurring receptor‐level antagonist of interleukin‐1 (IRAPorIL‐1 ra) has recently been cloned. To determine what stimuli might regulate this inhibitor, cytokines were tested for their effects on the steady‐state level of IRAP mRNA in phorbol ester‐differentiated U937 cells.
Susan O. Hankey, Donald B. Carter, Ann E. Berger, Robert N. McEwan   +3 more
openaire   +4 more sources

Treatment With an Interleukin-1 Receptor Antagonist Protein Prolongs Mouse Islet Allograft Survival

Diabetes, 1993
An interleukin-1 receptor antagonist protein was evaluated with regard to its efficacy in allogeneic and xenogeneic islet transplantation. Alloxan-induced diabetic C57BL/6 (H-2b) mice were transplanted under the kidney capsule with 500 C57BL/Ks (H-2d) mouse islets.
Sandberg, J O, Eizirik, Decio L., Sandler, S, Tracey, D E, Andersson, A.   +4 more
openaire   +4 more sources

Interleukin-1α and Interleukin-1β Stimulate Adrenocorticotropin Secretion in the Rat through a Similar Hypothalamic Receptor(s): Effects of Interleukin-1 Receptor Antagonist Protein

Neuroendocrinology, 1993
Numerous reports have demonstrated that interleukin-1 beta (IL-1 beta) is a potent secretagogue for adrenocorticotropin (ACTH) and that IL-1 alpha appears to be considerably less efficacious. To clarify apparent differences in the potency of IL-1 alpha vs. -beta on ACTH secretion from a functional perspective, the IL-1 receptor antagonist protein, IRAP,
Shannon G. Matta, Burt M. Sharp, Kristin M. Linner   +2 more
openaire   +4 more sources

Cloning, heterologous expression and characterization of murine interleukin 1 receptor antagonist protein

European Journal of Immunology, 1991
AbstractA cDNA coding for the human interleukin 1 receptor antagonist protein (IL 1Ra) was used to clone the corresponding murine cDNA. The nucleotide sequence of the open reading frame coding for the processed form of mIL 1Ra predicted a 152‐residue protein that was 77% identical to human IL 1Ra.
Mary E. Shuck, Daniel E. Tracey, Michael J. Bienkowski, Thomas E. Eessalu   +3 more
openaire   +4 more sources

Potential agonist action of the interleukin-1 receptor antagonist protein: implications for treatment of women.

The Journal of Clinical Endocrinology & Metabolism, 1993
The interleukin-1 receptor antagonist protein (IRAP) caused a concentration-related increase in prostaglandin production by human decidual cells. The degree and time course of this stimulatory action were identical to those induced by interleukin 1 beta (IL-1 beta).
Mitchell, M.D., Edwin, S.S., Silver, R.M., Romero, R.J.   +3 more
openaire   +5 more sources