Results 31 to 40 of about 112,531 (165)

A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli. [PDF]

Cell Reports, 2022
Nucleus-forming jumbo phages establish an intricate subcellular organization, enclosing phage genomes within a proteinaceous shell called the phage nucleus.
Pogliano, Joe, Thomas G. Laughlin, Erica A. Birkholz, Corbett, Kevin D, Villa, Elizabeth, Suslov, Sergey, Armbruster, Emily, Lee, Jina, Birkholz, Erica A, Wittmann, Johannes, Johannes Wittmann, Emily Armbruster, Sergey Suslov, Kevin D. Corbett, Elizabeth Villa, Jina Lee, Laughlin, Thomas G, Joe Pogliano   +17 more
core   +5 more sources

The human oral phageome is highly diverse and rich in jumbo phages [PDF]

bioRxiv, 2020
Until recently, the contribution of bacteriophages to the composition and function of the human microbiome has been largely overlooked. Recent developments in discovering novel bacteriophages from human metagenomes have been mostly focused on the gut ...
Victoria R. Carr, A. Shkoporov, D. Gómez-Cabrero, P. Mullany, C. Hill, D. Moyes   +5 more
semanticscholar   +2 more sources

Isolation protocol of jumbo phage from winter grass soil [PDF]

IOP Conference Series: Earth and Environmental Science, 2021
Jumbo phage can isolated by two different methods and protocols. It depends on size, phage species, substrate sources, environment, media and tools. Each method has positive and negative results within each factors.
T KAWASAKI, T Kawasaki, SAAD, A Mohamed, WAFA, Ali, ADDY, H S   +3 more
core   +2 more sources

Molecular basis for anti-jumbo phage immunity by AVAST type 5.

Mol Cell
Jumbo phages protect their genomes from DNA-sensing bacterial defense systems by enclosing them within vesicles and nucleus-like compartments. Very little is known about defense systems specialized to counter these phages. Here, we show that AVAST Type 5
Muralidharan A, Costa AR, Fierlier D, van den Berg DF, van den Bossche H, Zoumaro-Djayoon AD, Rodríguez-Molina A, Pabst M, Pacesa M, Correia BE, Brouns SJJ.   +10 more
europepmc   +7 more sources

Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage

Applied Microbiology and Biotechnology, 2023
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields.
Soares, José Júnior Ferreira, da Silva Paes, Isabela, de Paula, Sérgio Oliveira, Xisto, Mariana Fonseca, Kropinski, Andrew M., da Silva Duarte, Vinícius, da Silva, Jéssica Duarte, Melo, Luís Daniel Rodrigues, Vieira, Marcella Silva, Santos, Sílvio Roberto Branco, Dias, Roberto Sousa, Porcellato, Davide   +11 more
core   +4 more sources

Phage isolation and functional characterization reveal strong antibiofilm activity against Pseudomonas aeruginosa in a cystic fibrosis sputum model [PDF]

Frontiers in Cellular and Infection Microbiology
Pseudomonas aeruginosa is an opportunistic pathogen that forms persistent biofilms in the lungs of cystic fibrosis and other chronic pulmonary disease patients, contributing to antibiotic tolerance, recurrent infection, and clinical decline.
Madeline Bowder, Hannah Kapoor, Steven J Carrell, Lia Danelishvili, Lia Danelishvili   +4 more
doaj   +2 more sources

Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage [PDF]

Science Advances, 2022
Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm.
Pogliano, Joe, Chaikeeratisak, Vorrapon, Nguyen, Katrina T., Enustun, Eray, Nguyen, Katrina T, Egan, MacKennon E, Villa, Elizabeth, Armbruster, Emily, Lee, Jina, Pogliano, Kit, Egan, MacKennon E., Khanna, Kanika   +11 more
core   +5 more sources

Characterisation of a Novel Jumbo Lytic Aeromonas dhakensis Bacteriophage P19 and Its Endolysin. [PDF]

Microb Biotechnol
A jumbo, lytic myovirus phage P19 against multidrug‐resistant (MDR) Aeromonas dhakensis was isolated from river water. P19 demonstrated robust lytic activity, high thermal and pH stability (pH 6–10, up to 60°C), and no genes associated with lysogeny, virulence or antimicrobial resistance. ORF_358 of P19 encodes for T4‐type lysozyme.
Ansari F, Nagar V.
europepmc   +2 more sources

The Dynamics of Synthesis and Localization of Jumbo Phage RNA Polymerases inside Infected Cells

Viruses, 2023
A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP)
Daria Antonova, Viktoriia V. Belousova, Erik Zhivkoplias, Mariia Sobinina, Tatyana Artamonova, Innokentii E. Vishnyakov, Inna Kurdyumova, Anatoly Arseniev, Natalia Morozova, Konstantin Severinov, Mikhail Khodorkovskii, Maria V. Yakunina   +11 more
doaj   +3 more sources

A phage nucleus-associated RNA-binding protein is required for jumbo phage infection

Nucleic Acids Research
Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and DNA-targeting CRISPR-Cas
Eray Enustun, Emily G. Armbruster, Jina Lee, Sitao Zhang, Brian A. Yee, Kseniya Malukhina, Yajie Gu, Amar Deep, Jack T Naritomi, Qishan Liang, S. Aigner, Benjamin A. Adler, Brady F. Cress, Jennifer A. Doudna, Vorrapon Chaikeeratisak, Don W. Cleveland, Majid Ghassemian, Bogdan Bintu, Gene W. Yeo, Joseph A. Pogliano, Kevin D. Corbett   +20 more
semanticscholar   +5 more sources