Results 101 to 110 of about 178,982 (291)

Generation of an acrAacrE double-knockout in Escherichia coli and its role in kanamycin resistance

, 2020
AcrAB and AcrEF are multidrug efflux pumps found in Escherichia coli that are thought to mediate the export of kanamycin, and therefore contribute to kanamycin resistance. Previous studies found that expression of acrE increased in response to increasing
Park, Cathy, Ang, Ada, Kumar, Shaneel, De Guzman, Josh   +3 more
core   +1 more source

Investigations of the Evolved Molecular Basis for Terpenoid Biosynthesis in Marine Sponges

Advanced Science, EarlyView.
Confirming and extending a previous observation in another Bubarida sponge, genomic and functional analyses of A. cavernosa reveal that sponges retain the mevalonate pathway and employ single α‐domain T1TSs and UbiA‐type TSs for terpenoid biosynthesis. The absence of T1TSs clustering with other biosynthetic genes tentatively suggests, based on limited ...
Fangyan Chen, Bao Chen, Wenhui Zhang, Guifang Sun, Yao Zhao, Jianpeng Zhang, Baofu Xu   +6 more
wiley   +1 more source

Development of immunoassays for the detection of kanamycin in veterinary fields [PDF]

, 2006
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk.
Jin-Wook Jang, Mun-Han Lee, Jin, Yong, Yong Jin, Jang, Jin-Wook, Lee, Mun Han, Han, Chang-Hoon, Chang-Hoon Han   +7 more
core   +1 more source

The Mitogen‐Activated Protein Kinase GhNTF3 Modulates the Arbuscular Mycorrhizal Symbiosis‐Immunity Trade‐Off in Cotton by Regulating Salicylic Acid Biosynthesis

Advanced Science, EarlyView.
AM symbiosis induces GhWAK13 to promote fungal colonization. GhNTF3 negatively regulates symbiosis by promoting SA biosynthesis. GhWAK13 sequesters GhNTF3 at the membrane, limiting its nuclear translocation and relieving SA‐mediated suppression. Under Verticillium stress, GhNTF3 enters the nucleus and interacts with GhJAZ6 to activate SA signaling ...
Shuangjie Jia, Jingshang Wen, Jihang Li, Meina Feng, Yiming He, Ruojun Liu, Qian Liu, Junli Zhang, Kai Cheng, Xiangyu Zhang, Xiao Zhang   +10 more
wiley   +1 more source

A Conserved Isoleucine Gates the Diffusion of CO and O2 to the Active Site of NiFe CO‐Dehydrogenase

Angewandte Chemie, EarlyView.
A combination of protein film electrochemistry and site‐directed mutagenesis shows that isoleucine 563 (numbering of Thermococcus sp. AM4) gates the arrival of both CO and O2 to the active site of the nickel‐iron CO dehydrogenases. Mutations at this position increase the resistance to O2 at the cost of a slower reaction with CO.
Laura Opdam, Marta Meneghello, Chloé Guendon, Jade Chargelègue, Andrea Fasano, Aurore Jacq‐Bailly, Christophe Léger, Vincent Fourmond   +7 more
wiley   +2 more sources

Detection of Resistance to Fluoroquinolones and Second-Line Injectable Drugs Among Mycobacterium tuberculosis by a Reverse Dot Blot Hybridization Assay

Infection and Drug Resistance, 2020
Guilian Li,1,* Qian Guo,1,2,* Haican Liu,1 Li Wan,1 Yi Jiang,1 Machao Li,1 Li-li Zhao,1 Xiuqin Zhao,1 Zhiguang Liu,1 Kanglin Wan1 1State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and ...
Li G, Guo Q, Liu H, Wan L, Jiang Y, Li M, Zhao LL, Zhao X, Liu Z, Wan K   +9 more
doaj  

Effect of Kanamycin Sulfate and Gentamicin on Growth of Probiotics

, 2011
Effect of kanamycin sulfate and gentamicin on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus bulgaricus was studied by measuring the optical density at 600nm and pH using MRS ...
Zhao Wei Wang, Guo Wei Shu, Zhe Ji, He Chen   +3 more
core   +1 more source

One‐Step Immobilized Phage Depolymerase Coatings for Catheter‐Related Bloodstream Infections

Advanced Science, EarlyView.
Catheter‐related bloodstream infections (CRBSI) caused by drug‐resistant Gram‐negative bacteria remain a major clinical challenge. Here, a polydopamine‐assisted depolymerase coating degrades bacterial capsules and restores serum‐mediated killing, effectively suppressing biofilms and reducing bacterial burden in vitro, ex vivo, and in vivo, presenting a
Lingkai Dong, Xingjin Li, Tao Hu, Qianqian Lu, Xiaoxu Zhang, Xi Chen, Yifan Luo, Zhong Zuo, Ronald Man Yeung Wong, Miao Xu, Tiancong Zhao, Sharon Shui Yee Leung   +11 more
wiley   +1 more source

Prestin-enhanced kanamycin-induced apoptosis depends on extracellular chloride.

, 2013
Control or prestin-transfected CHO cells were exposed to 100 µM kanamycin for 2 h in the presence or absence of extracellular chloride in KH media. Normal culture media F-12K was used as a control.
Xiao Hu Zhang (221708), Li Yu (81651), Zhen Zhou (184390), Lai Ling Tsang (221711), Hsiao Chang Chan (187453), Xiao Hua Jiang (355521), Mei Kuen Yu (187446), Yiu Wa Chung (355522), Hao Tang (44155), Ai Mei Wang (355523)   +9 more
core   +1 more source

Repurposing FDA‐Approved Drugs to Inhibit Fungal PPTases for Broad‐Spectrum Synergy

Advanced Science, EarlyView.
Fungal secondary metabolites serve as crucial virulence effectors; however, the potential of their biosynthetic pathways as antifungal targets remains inadequately comprehended. Repurposed FDA‐approved drugs inhibit fungal PPTase (essential for secondary metabolism) in vivo.
Zili Song, Can Zhao, Qiaoling Wang, Hongjiao Zhang, Xiao Liu, Linqi Wang, Michael Bromley, Wen‐Bing Yin   +7 more
wiley   +1 more source