Results 221 to 230 of about 178,982 (291)

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Correlation between antimicrobial susceptibility data and clinical efficacy for <i>Helicobacter cinaedi</i> bacteraemia. [PDF]

JAC Antimicrob Resist
Araoka H, Tomida J, Yoshino C, Baba M, Ogura S, Kimura M, Kawamura Y.   +6 more
europepmc   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Design and validation of an inducible and curable EvolvR system for directed evolution in <i>Corynebacterium glutamicum</i>. [PDF]

Front Bioeng Biotechnol
Will PM, Göttl VL, Seeger J, Wendisch VF, Henke NA.   +4 more
europepmc   +1 more source

High‐Contiguity Haplotype‐Resolved Genome Assembly of the Hexaploid Actinidia valvata Rootstock Sheds Light on Waterlogging Resistance Gene

Plant Biotechnology Journal, EarlyView.
ABSTRACT Polyploidisation, creating redundant or diverged copies of the genome, is a major driving force in plant evolution, diversification and environmental adaptation, including for the kiwifruit genus (Actinidia Lindl.). We present a high‐contiguity, haplotype‐resolved genome assembly of the hexaploid Actinidia valvata rootstock cultivar ...
Miaomiao Lin, Zhi Li, Cecilia H. Deng, Congcong Li, Zhengzhen Zhang, Yukuo Li, Qina Zhang, Peng Zhang, Leiming Sun, Mingyu Liu, Jinbao Fang, Xiujuan Qi   +11 more
wiley   +1 more source

Therapeutic potential of mitochondrial transfer in reversing mutant-to-wild-type mtDNA ratio and improving mitochondrial dysfunction in 1555A>G mtDNA mutation-associated hearing loss. [PDF]

Sci Rep
Kim Y, Kim CH, Nam DW, Kim BJ, Tran NT, Han JH, Kim M, Yu SH, Lee SE, Yeo JS, Kwon I, Han K, Kim CH, Kang YC, Choi BY.   +14 more
europepmc   +1 more source

Iterative Genome Engineering Platform Enables Efficient Sucrose Biosynthesis From CO2 in Photosynthetic Synechococcus elongatus UTEX 2973

Plant Biotechnology Journal, EarlyView.
ABSTRACT The single crossover occurring via homologous recombination is a common phenomenon existing among microbes like Escherichia coli, Bacillus subtilis, Vibrio natriegens, Gluconobacter oxydans and most cyanobacteria species, threatening the stability of engineered strains and challenging iterative genetic engineering. Among them, we take the fast‐
Shubin Li, Tao Sun, Dailin Liu, Tong Zhang, Lei Chen, Weiwen Zhang   +5 more
wiley   +1 more source

Efficient Embryogenic Callus Induction and <i>Agrobacterium</i>-Mediated Transformation of the Elite Foxtail Millet (<i>Setaria italica</i> L.) Variety Jingu51. [PDF]

Plants (Basel)
Zhang HH, Dai LJ, Xiao N, Zhou Y, Hu X, Zhang CH, Jia YY, Li M, Hao YS, Wu SJ.   +9 more
europepmc   +1 more source

Plant‐Based Production of Human Major Histocompatibility Complex Class II Molecules

Plant Biotechnology Journal, EarlyView.
ABSTRACT Major histocompatibility complex class II (MHC II) protein triggers adaptive immune responses by presenting antigens on antigen‐presenting cells (APCs) to be recognised by CD4+ T cells. Recombinant MHC II proteins are effective for the analysis of peptide‐binding interaction, detection of antigen‐specific T‐cells and clinical testing for donor‐
Abdelaziz Ramadan, Hiroko Miyadera, Kaho Oka, Kohei Ishikawa, Toshiya Kobayashi, Misaki Kobayashi, Mitsunori Shiroishi, Emiko Noguchi, Kenji Miura   +8 more
wiley   +1 more source

Evidence for a Constrained Mutational Pathway to High-Level Spectinomycin Resistance in <i>Neisseria</i>: RpsE Loop 2 Mutations and Associated Growth Costs. [PDF]

Int J Mol Sci
Kravtsov DV, Gryadunov DA, Anashkina AA, Shaskolskiy BL.   +3 more
europepmc   +1 more source