Building Immunocompetent Cerebral Organoids From a Developmental Perspective
Glia, EarlyView.Conventional cerebral organoids do not contain microglia, which must be added. We propose recommendations to assess microglial immunocompetence using quantitative approaches and stringent statistical analysis to help develop standardized protocols. ABSTRACT Cerebral organoids derived from human induced pluripotent stem cells (iPSCs) are increasingly ...
Xabier Cuesta‐Puente +8 more
wiley +1 more source
Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis
Scientific Reports, 2017 For faithful chromosome segregation, the formation of stable kinetochore–microtubule attachment and its monitoring by the spindle assembly checkpoint (SAC) are coordinately regulated by mechanisms that are currently ill-defined.
Masanori Ikeda, Kozo Tanaka
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Tubulin interaction with kinetochore proteins: analysis by in vitro assembly and chemical cross-linking. [PDF]
, 1987 R. Balczon, B. R. Brinkley
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Mechanisms of enhancer‐driven oncogene activation
International Journal of Cancer, EarlyView.Abstract An aggressive subtype of acute myeloid leukemia (AML) is caused by enhancer hijacking resulting in MECOM overexpression. Several chromosomal rearrangements can lead to this: the most common (inv(3)/t(3;3)) results in a hijacked GATA2 enhancer, and there are several atypical MECOM rearrangements involving enhancers from other hematopoietic ...
Joyce Vriend +2 more
wiley +1 more source
Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals
Cell Reports, 2022 Summary: Chromosome segregation in mammals relies on the maturation of a thick bundle of kinetochore-attached microtubules known as k-fiber. How k-fibers mature from initial kinetochore microtubule attachments remains a fundamental question. By combining
Ana C. Almeida +9 more
doaj
Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis. [PDF]
, 1994 Kevin D. Brown +3 more
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Deregulated enhancer‐promoter communication in cancer through altered nuclear architecture
International Journal of Cancer, EarlyView.Abstract Enhancers are critical regulators of gene expression. Structural variations in cancer genomes can lead to enhancer hijacking, where oncogenes are activated by mistargeted enhancer activity. Novel enhancer‐promoter interactions may also arise through chromosomal rearrangements that create extrachromosomal DNA elements.
Isabelle Seufert +3 more
wiley +1 more source
Chromosome motion during attachment to the vertebrate spindle: initial saltatory-like behavior of chromosomes and quantitative analysis of force production by nascent kinetochore fibers. [PDF]
, 1991 Stephanie Alexander, C L Rieder
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iLABMED, EarlyView.Extrachromosomal circular DNA (eccDNA) has quite recently turned out to be a cardinal component in cardiovascular diseases (CVD), governing the gene expression and mechanisms of disease causation. Studies indicated an upregulation of eccDNA in pulmonary arterial hypertension (PAH), which makes it strikingly potential as a sensitive and specific ...
Saim Mahmood Khan +6 more
wiley +1 more source
Microinjection of biotin-tubulin into anaphase cells induces transient elongation of kinetochore microtubules and reversal of chromosome-to-pole motion. [PDF]
, 1992 Eric A. Shelden, Patricia Wadsworth
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