Results 131 to 140 of about 1,352 (160)

Up‐regulation of atrial and neuronal Kir3 activity by cholesterol

The FASEB Journal, 2017
Cholesterol is one of the major lipid components of the plasma membrane in mammalian cells, and its excess is associated with multiple pathological conditions including cardiovascular and neurodegenerative disease. In recent years, cholesterol has been emerging as a major regulator of ion channel function.
Avia Rosenhouse‐Dantsker, Anna N Bukiya   +1 more
openaire   +1 more source

Interaction of G Protein β Subunit with Inward Rectifier K+ Channel Kir3

Molecular Pharmacology, 2003
G protein betagamma subunits bind and activate G protein-coupled inward rectifier K+ (GIRK) channels. This protein-protein interaction is crucial for slow hyperpolarizations of cardiac myocytes and neurons. The crystal structure of Gbeta shows a seven-bladed propeller with four beta strands in each blade.
Qi, Zhao, Takeharu, Kawano, Hiroko, Nakata, Yasuko, Nakajima, Shigehiro, Nakajima, Tohru, Kozasa   +5 more
openaire   +2 more sources

A novel, radiolabel-free pulse chase strategy to study Kir3 channel ontogeny

Journal of Receptors and Signal Transduction, 2013
Kir3 channels are essential regulators of cellular excitability, maintaining cells at resting membrane potentials. While much research has been dedicated to elucidating the mechanisms regulating Kir3 channel gating, little is known regarding the channel's early associations with signaling partners, its stability at the plasma membrane or mechanisms ...
Peter, Zylbergold, Rory, Sleno, Terence E, Hébert   +2 more
openaire   +2 more sources

Interaction of Gαq and Kir3, G Protein-Coupled Inwardly Rectifying Potassium Channels

Molecular Pharmacology, 2007
Activation of substance P receptors, which are coupled to Galpha(q), inhibits the Kir3.1/3.2 channels, resulting in neuronal excitation. We have shown previously that this channel inactivation is not caused by reduction of the phosphatidylinositol 4,5-bisphosphate level in membrane.
Takeharu, Kawano, Peng, Zhao, Christina V, Floreani, Yasuko, Nakajima, Tohru, Kozasa, Shigehiro, Nakajima   +5 more
openaire   +2 more sources

DNA Origami Scaffolds as Templates for Functional Tetrameric Kir3 K⁺ Channels

Angewandte Chemie, 2018
AbstractIn native systems, scaffolding proteins play important roles in assembling proteins into complexes to transduce signals. This concept is yet to be applied to the assembly of functional transmembrane protein complexes in artificial systems.
Tatsuki, Kurokawa, Shigeki, Kiyonaka, Eiji, Nakata, Masayuki, Endo, Shohei, Koyama, Emiko, Mori, Nam Ha, Tran, Huyen, Dinh, Yuki, Suzuki, Kumi, Hidaka, Masaaki, Kawata, Chikara, Sato, Hiroshi, Sugiyama, Takashi, Morii, Yasuo, Mori   +14 more
openaire   +2 more sources

Kir3 channels undergo arrestin-dependant internalization following delta opioid receptor activation

Cellular and Molecular Life Sciences, 2015
Kir3 channels control excitability in the nervous system and the heart. Their surface expression is strictly regulated, but mechanisms responsible for channel removal from the membrane remain incompletely understood. Using transfected cells, we show that Kir3.1/3.2 channels and delta opioid receptors (DORs) associate in a complex which persists during ...
Karim, Nagi, Iness, Charfi, Graciela, Pineyro   +2 more
openaire   +2 more sources

Co-expression of Human Kir3 Subunits Can Yield Channels with Different Functional Properties

Cellular Signalling, 1999
To date, no comprehensive study has been done on all combinations of the human homologues of the Kir3.0 channel family, and the human homologue of Kir3.3 has not yet been identified. To obtain support for the contention that most of the functional data on non-human Kir3.0 channels can be extrapolated to human channels, we have cloned the human ...
O, Schoots, J M, Wilson, N, Ethier, E, Bigras, T E, Hebert, H H, Van Tol   +5 more
openaire   +2 more sources

Heterogeneous Distribution of Kir3 Potassium Channel Proteins Within Dopaminergic Neurons in the Mesencephalon of the Rat Brain

Cellular and Molecular Neurobiology, 2007
1. Dopaminergic neurons in the substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA) of the ventral mesencephalon play an important role in the regulation of the parallel basal ganglia loops.2. We have raised affinity-purified polyclonal rabbit antibodies specific for all four members of the Kir3 family of inwardly rectifying ...
Dirk, Eulitz, Harald, Prüss, Christian, Derst, Rüdiger W, Veh   +3 more
openaire   +2 more sources

Design and construction of conformational biosensors to monitor ion channel activation: A prototype FlAsH/BRET-approach to Kir3 channels

Methods, 2016
Ion channels play a vital role in numerous physiological functions and drugs that target them are actively pursued for development of novel therapeutic agents. Here we report a means for monitoring in real time the conformational changes undergone by channel proteins upon exposure to pharmacological stimuli.
Derek N, Robertson, Rory, Sleno, Karim, Nagi, Darlaine, Pétrin, Terence E, Hébert, Graciela, Pineyro   +5 more
openaire   +2 more sources

Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury

2020
Patch clamp is an electrophysiological technique that allows to analyze the activity of ion channels in neurons. In this chapter, we provide a detailed description of patch clamp protocol to measure the effect of a μ-opioid receptor agonist on the activity of G protein-coupled inwardly rectifying potassium (GIRK or Kir3) channels.
Viola, Seitz, Philip, Stötzner, Dominika, Labuz, Halina, Machelska   +3 more
openaire   +2 more sources