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Kluyveromyces lactis: An emerging tool in biotechnology
Journal of Biotechnology, 2016Kluyveromyces lactis has emerged as one of the most important yeast species for research and industrial biotechnology. This Crabtree-negative species is suitable for the production of metabolites and heterologous proteins, and its ability to achieve high levels of protein secretion makes it an attractive alternative for industrial protein production ...
Sebastian C, Spohner +3 more
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Flocculation Dispersion in Kluyveromyces lactis
Process Biochemistry, 1995Yeast flocculation is a complex phenomenon. The present work concerns the effect of different factors on the flocculation dispersion of Kluyveromyces lactis. This study employs mother and hybrid strains, the latter being obtained by mating a flocculent and a non-flocculent mother strain. The optimum pH of flocculation was around 4·5.
Bellal, Mouloud +3 more
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Nucleosome organization on Kluyveromyces lactis centromeric DNAs
Biophysical Chemistry, 2002The preferential assembly of specialized nucleosomes on budding yeast centromeres can be due either to the higher stability of specialized centromeric nucleosomes and/or to the lower stability of canonical centromeric nucleosomes with respect to bulk nucleosomes.
Mattei S +3 more
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Transformation of Kluyveromyces lactis
2003Often the aim of a transformation is to obtain at least one transformant that can be used in further experiments. This transformant should contain either a linear fragment integrated at the correct position in the genome or a plasmid. On the other hand, for transformation with a plasmid library, for example, high transformation efficiency is required ...
Rolf A. Kooistra, H. Yde Steensma
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A Novel Regulated Hybrid Promoter That Permits Autoinduction of Heterologous Protein Expression in Kluyveromyces lactis [PDF]
Applied and Environmental Microbiology, 2019 Christopher H Taron, Juliane Behler
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The proteolytic system of the yeast Kluyveromyces lactis
Yeast, 1999Major proteolytic activities were characterized in the yeast K. lactis NRRL 1118, grown in chemostat cultures. This yeast expressed proteolytic activities similar to those found in S. cerevisiae. This fact was particularly evident in the case of proteases such as PrA, PrB and CpY with regard to substrate specificity, activation at pH 5.
M V, Flores, A, Cuellas, C E, Voget
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Expression cloning in Kluyveromyces lactis
Biotechnology Techniques, 1999Kluyveromyces lactis was used as host for an Aspergillus tubingensis expression library. A new episomal vector was constructed to direct the expression of the A. tubingensis cDNAs and to allow subsequent analysis in Escherichia coli. Using three different plate assays, 18000 K.
van der Vlugt-Bergmans, C.J.B. +1 more
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The UPC2 gene in Kluyveromyces lactis stress adaptation
Folia Microbiologica, 2022KlUpc2p, a transcription factor belonging to the fungal binuclear cluster family, is an important regulator of ergosterol biosynthesis and azole drug resistance in Kluyveromyces lactis. In this work, we show that the absence of KlUpc2p generates Rag- phenotype and modulates the K. lactis susceptibility to oxidants and calcofuor white.
Veronika Betinova +4 more
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Molecular biology of Kluyveromyces lactis
1993The yeast Saccharomyces cerevisiae is probably the most thoroughly understood amongst eukaryotic organisms and an excellent model for the study of eukaryotic cells in general; indeed, the term “yeast” is often used as a synonim for this species. Recently, however, other yeasts have attracted the attention of researchers as a result of their distinct ...
FRONTALI, Laura +7 more
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Synonymous codon usage in Kluyveromyces lactis
Yeast, 1993AbstractThe nature and variation of synonymous codon usage in 47 open reading frames from Kluyveromyces lactis have been investigated. Using multivariate statistical analysis, a single major trend among K. lactis genes was identified that differentiates among genes by expression level: highly expressed genes have high codon usage bias, while genes of ...
A T, Lloyd, P M, Sharp
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