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Flocculation Dispersion in Kluyveromyces lactis
Process Biochemistry, 1995Yeast flocculation is a complex phenomenon. The present work concerns the effect of different factors on the flocculation dispersion of Kluyveromyces lactis. This study employs mother and hybrid strains, the latter being obtained by mating a flocculent and a non-flocculent mother strain. The optimum pH of flocculation was around 4·5.
Bellal, Mouloud +3 more
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Centromeric DNA of Kluyveromyces lactis
Current Genetics, 1990A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K.
J J, Heus +3 more
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Nucleosome organization on Kluyveromyces lactis centromeric DNAs
Biophysical Chemistry, 2002The preferential assembly of specialized nucleosomes on budding yeast centromeres can be due either to the higher stability of specialized centromeric nucleosomes and/or to the lower stability of canonical centromeric nucleosomes with respect to bulk nucleosomes.
Mattei S +3 more
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Expression cloning in Kluyveromyces lactis
Biotechnology Techniques, 1999Kluyveromyces lactis was used as host for an Aspergillus tubingensis expression library. A new episomal vector was constructed to direct the expression of the A. tubingensis cDNAs and to allow subsequent analysis in Escherichia coli. Using three different plate assays, 18000 K.
van der Vlugt-Bergmans, C.J.B. +1 more
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Heme-mediated transcriptional control in Kluyveromyces lactis
Current Genetics, 2000Heme is of great importance in oxygen-dependent biological functions, since it serves as a prosthetic group for many proteins related to oxygen-binding, oxidative damage prevention and electron transport. It also regulates gene expression through the action of specific transcriptional regulatory factors.
M, González-Domínguez +4 more
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Kluyveromyces lactis: An emerging tool in biotechnology
Journal of Biotechnology, 2016Kluyveromyces lactis has emerged as one of the most important yeast species for research and industrial biotechnology. This Crabtree-negative species is suitable for the production of metabolites and heterologous proteins, and its ability to achieve high levels of protein secretion makes it an attractive alternative for industrial protein production ...
Sebastian C, Spohner +3 more
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Synonymous codon usage in Kluyveromyces lactis
Yeast, 1993AbstractThe nature and variation of synonymous codon usage in 47 open reading frames from Kluyveromyces lactis have been investigated. Using multivariate statistical analysis, a single major trend among K. lactis genes was identified that differentiates among genes by expression level: highly expressed genes have high codon usage bias, while genes of ...
A T, Lloyd, P M, Sharp
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Properties of mitochondrial DNA from Kluyveromyces lactis
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974Abstract 1. We have isolated a closed circular DNA fraction from mitochondria purified from Kluyveromyces lactis by centrifuging a mitochondrial lysate to equilibrium in CsCl containing ethidium bromide. Electron micrographs of appropriate gradient fractions show predominantly circular duplex DNA with an average contour length ( ± S.D.
J P, Sanders +3 more
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