Results 181 to 190 of about 13,811 (216)

Transformation of Kluyveromyces lactis

2003
Often the aim of a transformation is to obtain at least one transformant that can be used in further experiments. This transformant should contain either a linear fragment integrated at the correct position in the genome or a plasmid. On the other hand, for transformation with a plasmid library, for example, high transformation efficiency is required ...
Rolf A. Kooistra, H. Yde Steensma
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Efficient gene targeting in Kluyveromyces lactis

Yeast, 2004
AbstractIntegration of a DNA fragment in a host genome requires the action of a double‐strand break (DSB) repair mechanism. Homologous recombination (HR) is initiated by binding of Rad52p to DNA ends and results in targeted integration. Binding of the Ku heterodimer (Ku70p/Ku80p) results in random integration via non‐homologous end joining (NHEJ).
Rolf, Kooistra, Paul J J, Hooykaas, H Yde, Steensma   +2 more
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Finding of dairy yeasts Kluyveromyces lactis var. lactis in natural habitats

Microbiology, 2014
Well-known yeasts Kluyveromyces lactis var. lactis, which are usually associated with dairy prod- ucts, were discovered in nature (in woodland park soil under Impatiens glandulifera Royle plants). Reliable identification of the yeasts was carried out using physiological criteria (lactose and maltose utilization) and molecular markers (nucleotide ...
G I, Naumov, E S, Naumova, A M, Glushakova, A V, Kachalkin, I Y, Chernov   +4 more
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Molecular biology of Kluyveromyces lactis

1993
The yeast Saccharomyces cerevisiae is probably the most thoroughly understood amongst eukaryotic organisms and an excellent model for the study of eukaryotic cells in general; indeed, the term “yeast” is often used as a synonim for this species. Recently, however, other yeasts have attracted the attention of researchers as a result of their distinct ...
FRONTALI, Laura, BIANCHI, Michele Maria, FABIANI, Lucia, FALCONE, Claudio, MAZZONI, Cristina, RAGNINI A., SALIOLA, Michele, WILSON C.   +7 more
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Regulation of primary carbon metabolism in Kluyveromyces lactis

Enzyme and Microbial Technology, 2000
In the recent past, through advances in development of genetic tools, the budding yeast Kluyveromyces lactis has become a model system for studies on molecular physiology of so-called "Nonconventional Yeasts." The regulation of primary carbon metabolism in K.
, Breunig, , Bolotin-Fukuhara, , Bianchi, , Bourgarel, , Falcone, , Ferrero, , Frontali, , Goffrini, , Krijger, , Mazzoni, , Milkowski, , Steensma, , Wésolowski-Louvel, , Zeeman   +13 more
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Deletions and rearrangements in Kluyveromyces lactis mitochondrial DNA

Current Genetics, 1989
Three classes of respiratory deficient mutants have been isolated from a fusant between Kluyveromyces lactis and Saccharomyces cerevisiae that contains only K. lactis mtDNA. One class (15 isolates), resemble rho 0 mutants of S. cerevisiae as they lack detectable mtDNA.
C M, Hardy, C L, Galeotti, G D, Clark-Walker   +2 more
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Electroinduced extraction of β-galactosidase from Kluyveromyces lactis

Applied Microbiology and Biotechnology, 2001
A new methodology for the extraction of beta-galactosidase from the yeast Kluyveromyces lactis was obtained by electropulsation. The application of a series of electric pulses (2 ms duration, 1 Hz frequency, and 4-4.5 kV/cm field strength) to fresh cells suspended in deionized water, followed by incubation in PBS, led to a spontaneous slow release of ...
V, Ganeva, B, Galutzov, N, Eynard, J, Teissié   +3 more
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Action spectrum of Kluyveromyces lactis mycocins

Microbiology, 2013
New mycocinogenic strains of the yeast Kluyveromyces lactis were found. They have fungicidic activity at pH from 5 to 7. This activity was eliminated by UV irradiation. Among over 260 species tested, ones sensitive to these mycocins were revealed mainly in the families Saccharomycetaceae and Wickerhamomycetaceae of the order Saccharomycetales.
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Magnification of the rDNA cluster in Kluyveromyces lactis

Molecular and General Genetics MGG, 1990
By employing pulsed field gel electrophoresis we find that slow growing strains of Kluyveromyces lactis have only 43%-55% of the wild-type level of ribosomal DNA (rDNA) repeats. When subjected to prolonged vegetative growth these strains can increase both the number of rDNA repeats and their growth rate.
R, Maleszka, G D, Clark-Walker
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Kluyveromyces lactis: genetics, physiology, and application

2003
For Kluyveromyces lactis basic research, development of genetic tools and industrial application has gone hand in hand over the last fifteen years. This article summarizes the current knowledge with particular emphasis on genetics and physiology in comparison to Saccharomyces cerevisiae. The K.
Karin D. Breunig, H. Yde Steensma
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