Results 71 to 80 of about 7,704 (210)

Pathways for glutamate biosynthesis in the yeast Kluyveromyces lactis [PDF]

Microbiology, 2000
Purified glutamate synthase (GOGAT) from Kluyveromyces lactis was characterized as a high-molecular-mass polypeptide, a distinction shared with previously described GOGATs from other eukaryotic micro-organisms. Using degenerate deoxyoligonucleotides, designed from conserved regions of the alfalfa, maize and Escherichia coli GOGAT genes, a 300 bp PCR ...
Mauricio, Romero, Simón, Guzmán-León, Cristina, Aranda, Diego, González-Halphen, Lourdes, Valenzuela, Alicia, González   +5 more
openaire   +2 more sources

Safety of lacto‐N‐triose II as a novel food pursuant to Regulation (EU) 2015/2283

EFSA Journal, Volume 24, Issue 7, July 2026.
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on lacto‐N‐triose II (LNTri II) as a novel food (NF) pursuant to Regulation (EU) 2015/2283.
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Dominique Turck, Torsten Bohn, Montaña Cámara, Jacqueline Castenmiller, Stefaan De Henauw, Ángeles Jos, Alexandre Maciuk, Inge Mangelsdorf, Breige McNulty, Androniki Naska, Kristina Pentieva, Alfonso Siani, Frank Thies, Margarita Aguilera‐Gómez, Thomas Frenzel, Montaña Cámara, Inge Mangelsdorf, Francesca Marcon, Harry J. McArdle, Peter Moldeus, Monika Neuhäuser‐Berthold, Estefanía Noriega Fernández, Carolina Ropero‐Pérez, Karen Ildico Hirsch‐Ernst   +25 more
wiley   +1 more source

Nanoshell Fabrication Techniques for Probiotic Encapsulation: A Comprehensive Review

eFood, Volume 7, Issue 3, June 2026.
ABSTRACT Probiotics, beneficial microorganisms, confer numerous health benefits when administered. However, their efficacy is hindered by harsh gut conditions, including acidity, bile acids, and enzymatic degradation. To overcome these challenges, encapsulation and nanoshell technology have emerged as a pivotal strategy to enhance probiotic stability ...
Tuba Mustafa, Muhammad Afzaal, Farhan Saeed, Amna Shahbaz, Zainab Hira, Ayesha Adil, Tayyaba Sohail, Faiyaz Ahmed, Maryam Amanullah, Hina Sarwar, Noor Akram, Saad Mansoor, Gebremichael Gebremedhin Hailu   +12 more
wiley   +1 more source

Improved secretory production of calf prochymosin by codon optimization and disruption of PMR1 in Kluyveromyces lactis GG799 [PDF]

, 2013
Chymosin as an important industrial enzyme used widely in cheese manufacture. In our preliminary study, low yields (80 U mL-1) were obtained when Kluyveromyces lactis GG799 was used to express chymosin.
Ren, J, Feng, Z, Zhang, Y, Hu, S
core   +2 more sources

Transformation of Kluyveromyces lactis by killer plasmid DNA [PDF]

Journal of Bacteriology, 1983
Some strains of Kluyveromyces lactis contain two linear double-stranded DNA plasmids, k1 and k2. The presence of the two plasmids confer on the cell a "killer" character, due to the production of a toxin that kills the sensitive cells. We have used one of these linear DNA molecules as a gene vector to transform K. lactis cells.
de Louvencourt, L., Fukuhara, H., Heslot, H., Wesolowski, M.   +3 more
openaire   +3 more sources

Kefir: A Potential Gut Microbiota Modulator: A Systematic Review of Human Interventional Studies

MicrobiologyOpen, Volume 15, Issue 3, June 2026.
Daily cow's milk kefir intake transiently enriches gut microbiota, modulates the intestinal environment by increasing SCFA and reducing pH and oxygen, and exerts antimicrobial effects via SCFAs, bacteriocins, exopolysaccharides, and microbial fractions, highlighting its potential for gut health promotion.
Mohammed Hamsho, Yazan Ranneh, Batoul Kaddour, Sundus Alfakhri, Hale Hacıbayram, Wijdan Shkorfu, Merve Terzi, Abdulmannan Fadel   +7 more
wiley   +1 more source

Enzimas pectinolíticas de Kluyveromyces marxianus: seleção e aplicação em processos da indústria de alimentos [PDF]

, 2012
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de AlimentosNesse trabalho, dezenove leveduras do gênero Kluyveromyces foram testadas quanto à sua capacidade de produzir pectinases ...
Barreto, Luciani Tatsch Piemolini
core  

Polyethylenimine-Assisted Aggregation of β-Galactosidase from Kluyveromyces lactis

Hittite Journal of Science and Engineering
Using PEI as an aggregating agent with β-galactosidase from Kluyveromyces lactis was investigated with no solid support. PEI, a cationic polymer initiated instant aggregation with the enzyme in solution. The factors affecting the
Nedim Albayrak, Rabia Akyol Kütük
doaj   +1 more source

Improved Gene Targeting through Cell Cycle Synchronization. [PDF]

PLoS ONE, 2015
Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during ...
Vasiliki Tsakraklides, Elena Brevnova, Gregory Stephanopoulos, A Joe Shaw   +3 more
doaj   +1 more source

Fermentation and Enzymatic Pre‐Treatments to Modulate Sensory Properties of Extruded Plant‐Based Meat Alternatives

Sustainable Food Proteins, Volume 4, Issue 2, June 2026.
Fermentation, enzymatic hydrolysis, and enzymatic crosslinking are emerging pre‐treatments to improve the sensory properties of extruded plant‐based meat alternatives. This review critically examines current literature to identify underlying mechanisms, benefits, and key research gaps associated with these pre‐treatments.
Ravinder Singh, Michel Aliani, Warren Blunt, Filiz Koksel   +3 more
wiley   +1 more source