Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules [PDF]
Nature Communications, 2019 AbstractAssembly of soluble peptide-major histocompatibility complex class II (pMHCII) monomers into multimeric structures enables the detection of antigen-specific CD4+ T cells in biological samples and, in some configurations, their reprogramming in vivo.
Pau Serra +13 more
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Structural and Functional Characterization of a Hole–Hole Homodimer Variant in a “Knob-Into-Hole” Bispecific Antibody [PDF]
Analytical Chemistry, 2017 Bispecific antibodies have great potential to be the next-generation biotherapeutics due to their ability to simultaneously recognize two different targets. Compared to conventional monoclonal antibodies, knob-into-hole bispecific antibodies face unique challenges in production and characterization due to the increase in variant possibilities, such as ...
Hui‐Min Zhang +12 more
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Functional Coiled-Coil-like Assembly by Knob-into-Hole Packing of Single Heptad Repeat
ACS Nano, 2019 Coiled-coil peptides represent the principal building blocks for structure-based design of bionanomaterials. The sequence-structure relationship and precise nanoscale ordering of the coiled-coil helices originate from the knob-into-hole (KIH) packing of side chains.
Sudipta Mondal +5 more
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Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units [PDF]
Journal of Biological EngineeringDuring the production of bispecific antibodies (bsAbs), nonspecific pairing results in low yields of target bsAb molecules, an issue known as the “mispairing problem.” Several antibody engineering techniques have been developed to overcome mispairing ...
Jyunna Yoshida +7 more
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Physicochemical and biological characterization of a bispecific antibody in a CrossMab/KIH format that targets EGFR and VEGF-A [PDF]
Frontiers in ImmunologyIntroductionBispecific antibodies (BsAbs) are a class of antibody therapeutics engineered in various molecular formats to bind two distinct antigens and potentially mediate multiple biological effects.
Safiat Ayinde +3 more
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Aglycosylated knob and hole Fc homodimers are anti-parallel [PDF]
Protein engineering, design & selection : PEDS, 2014 An increasing number of bispecific therapeutic antibodies are progressing through clinical development. The Knob-into-Hole (KiH) technology uses complementary mutations in the CH3 region of the antibody Fc fragment to achieve heavy chain heterodimerization.
Mark Ultsch +7 more
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Rapid affinity-based purification of multi-specific antibodies using Kappa Select and Protein L [PDF]
mAbsMultispecific antibodies (msAbs) are becoming more prevalent as formats of choice for therapeutic antibody development due to their ability to modulate multiple biological targets.
Kalie Mix +6 more
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Rapid Generation of Murine Bispecific Antibodies Using FAST-IgTM for Preclinical Screening of HER2/CD3 T-Cell Engagers [PDF]
AntibodiesBispecific antibodies (BsAbs) can bind to two different antigens, enabling therapeutic concepts that cannot be achieved with monoclonal antibodies. Immuno-competent mice are essential for validating drug discovery concepts, necessitating the development ...
Hikaru Koga +5 more
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Journal of Materials Chemistry BPeptides are highly attractive as nanocarriers for drug delivery and other biomedical applications due to their unique combination of biocompatibility, efficacy, safety, and versatility that are difficult to achieve with other nanocarrier types.
Monika Szefczyk +8 more
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