Results 41 to 50 of about 312,266 (316)

L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans [PDF]

Journal of Bacteriology, 1994
The previously cloned gene for L-(+)-lactate dehydrogenase (LDH) from Streptococcus mutans was mutagenized in vitro. An Escherichia coli transformant which expressed a thermolabile LDH activity was identified. The ldh(Ts) gene was introduced into S.
Jeffrey D. Hillman, Anping Chen, M J Duncan   +2 more
openaire   +3 more sources

Protein Conformational Landscapes and Catalysis. Influence of Active Site Conformations in the Reaction Catalyzed by L-Lactate Dehydrogenase.

ACS Catalysis, 2015
In the last decade L-Lactate Dehydrogenase (LDH) has become an extremely useful marker in both clinical diagnosis and in monitoring the course of many human diseases. It has been assumed from the 80s that the full catalytic process of LDH starts with the
K. Świderek, I. Tuñón, S. Martí, V. Moliner   +3 more
semanticscholar   +1 more source

Human d‐lactate dehydrogenase deficiency by LDHD mutation in a patient with neurological manifestations and mitochondrial complex IV deficiency

JIMD Reports, 2021
Background d‐lactate, one of the isomers of lactate, exists in a low concentration in healthy individuals and it can be oxidized to pyruvate catalyzed by d‐lactate dehydrogenase.
Anna Ka‐Yee Kwong, Sheila Suet‐Na Wong, Richard J. T. Rodenburg, Jan Smeitink, Godfrey Chi Fung Chan, Cheuk‐Wing Fung   +5 more
doaj   +1 more source

DIAGNOSTIC UTILITY OF SERUM LACTATE DEHYDROGENASE LEVELS (LDL) IN DIFFERENTIATING MEGALOBLASTIC ANEMIA FROM MYELODYSPLASTIC SYNDROMES IN PAKISTAN

Pakistan Armed Forces Medical Journal, 2021
Objective: To study the diagnostic utility of lactate dehydrogenase levels in differentiating megaloblastic anemia from myelodysplastic anemia in Pakistan. Study Design: Comparative cross-sectional study.
Faraz Ali Rana, Helen Mary Robert, Madiha Ilyas, Asad Mahmood, Muhammad Amir, Nabeela Khan   +5 more
doaj  

Cytochrome b2 from bakers' yeast (L-lactate dehydrogenase). A double-headed enzyme.

European Journal of Biochemistry, 1974
Bakers' yeast cytochrome b2 [lL-(+)-lactate dehydrogenase or l-(+)-lactate cytochrome c oxidoreductase], usually purified by crystallisation according to Appleby and Morton, was shown to be a degraded form of the enzyme, arising by a proteolytic ...
C. Jacq, F. Lederer
semanticscholar   +1 more source

Evidence that L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans [PDF]

Infection and Immunity, 1994
In order to construct an effector strain for the replacement therapy of dental caries, we wished to combine the properties of low-level acid production and high-level colonization potential in a strain of Streptococcus mutans. To this end, we made a deletion in the lactate dehydrogenase (LDH) gene cloned from the bacteriocin-producing S.
Seok-Woo Lee, M J Duncan, A. Chen, Jeffrey D. Hillman   +3 more
openaire   +2 more sources

DETERMINATION OF DIAGNOSTIC ACCURACY OF BIOCHEMICAL PARAMETERS (CRP, LDH & FERRITIN) IN THE DIAGNOSIS OF COVID-19 IN SUSPECTED COVID CASES

Pakistan Armed Forces Medical Journal, 2021
Objective: To determine the diagnostic accuracy of the Lactate Dehydrogenase, C-Reactive Protein and Ferritin in suspected patients of COVID-19. Study Design: Cross-sectional validation study.
Tayyaba Ashiq, Abdus Sattar, Nasir Uddin, Qamar Bashir, Sajida Shaheen, Aamir Ijaz   +5 more
doaj  

Purification and properties of L(+)-lactate dehydrogenase from potato tubers.

Biochemical Journal, 1972
1. A purification of l(+)-lactate dehydrogenase is described. 2. The final preparation is active with NADH and NADPH and with a number of keto acids, but evidence is presented to support the view that a single enzyme is involved. 3. NAD(+) showed product
D. Davies, S. Davies
semanticscholar   +1 more source

Functional inhibition of lactate dehydrogenase suppresses pancreatic adenocarcinoma progression

Clinical and Translational Medicine, 2021
Background Pancreatic adenocarcinoma (PAAD) a highly lethal malignancy. The current use of clinical parameters may not accurately predict the clinical outcome, which further renders the unsatisfactory therapeutic outcome.
Chien‐shan Cheng, Hor‐Yue Tan, Ning Wang, Lianyu Chen, Zhiqiang Meng, Zhen Chen, Yibin Feng   +6 more
doaj   +1 more source

Amperometric L-Lactate Biosensor Based upon a Gold Nanoparticles/Reduced Graphene Oxide/Polyallylamine Hydrochloride Modified Screen-Printed Graphite Electrode

Chemosensors, 2021
This work describes a novel L-lactate biosensor based on the immobilization of L-lactate dehydrogenase enzyme on the screen-printed electrode modified with a ternary composite based on gold nanoparticles, electrochemically-reduced graphene oxide, and ...
Oana-Maria Istrate, Lucian Rotariu, Camelia Bala   +2 more
doaj   +1 more source