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Fully integrated production of L-malic acid
2010L-malic acid is a dicarboxylic acid and an intermediate of cell metabolism. It belongs to a group of „bulk“ chemicals with estimated consumption of 40, 000 tons per year. It is widely distributed in nature where it occurs in very low concentrations. It can be prepared by extraction from natural fruit juices or by bioconversion of fumaric acid with an ...
Kovačević, Tatjana, Zelić, Bruno
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Transport of l(-)malic acid and other dicarboxylic acids in the yeast Candida sphaerica
Applied Microbiology and Biotechnology, 1989dl-Malic acid grown cells of Candida sphaerica (anamorph of Kluyveromyces marxianus) formed a saturable transport system that mediated accumulative transport of l(-)malic acid with the following kinetic parameters at pH 5.0: V max, 0.44 nmol l(-)malate·s-1 per milligram dry weight; K m ,0.1 mM l(-)malate.
Manuela Côrte-Real +2 more
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Production of L-malic acid by permeabilized yeast cells
2005L-malic acid is an important intermediate of cell metabolism. It is second most popular acidulant in the food industry and holds about 10% of this market1. Commercially it is produced by chemical synthesis via hydratation of fumaric acid that results to the racemic mixture or by the enzymatic process, which yields to the L-isomer of malic acid. In this
Vasić-Rački, Đurđa +1 more
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Fully integrated L-malic acid production process
2011Bioconversion of fumaric acid to L-malic acid was performed using biocatalyst fumarase from whole permeabilized baker’s yeast cells Saccharomyces cerevisiae in a continuous stirred tank reactor, with biomass recirculation and in situ product separation. The process of in situ product separation consisted of five processing steps: biotransformation, the
Pandurić, Nikola +2 more
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Highly efficient neutralizer-free l-malic acid production using engineered Saccharomyces cerevisiae
Bioresource Technology, 2023Yanfeng Liu, Guocheng Du, Long Liu
exaly
Expression of Escherichia coli malic enzyme gene in Zymomonas mobilis for production of malic acid
Journal of Biotechnology, 2022Virendra Swarup Bisaria +2 more
exaly
Metabolic Engineering of Trichoderma reesei for l-Malic Acid Production
Journal of Agricultural and Food Chemistry, 2023, Dongzhi Wei, Yumeng Chen
exaly

