Results 261 to 270 of about 148,747 (310)
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How Lac repressor finds lac operator in vitro
Journal of Molecular Biology, 1992Filter-binding and gel mobility shift assays were used to analyse the kinetics of the interaction of Lac repressor with lac operator. A comparison of the two techniques reveals that filter-binding assays with tetrameric Lac repressor have often been misinterpreted.
Benno MÜLLER-HILL, B MÜLLER-HILL
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A model for the binding of lac repressor to the lac operator
Molecular Biology Reports, 1976A model is suggested for the lac repressor binding to the lac operator in which the repressor polypeptide chain sequences from Gly 14 to Ala 32 and from Ala 53 to Leu 71 are involved in specific interaction with operator DNA. A correspondence between the protein and DNA sequences is found which explains specificity of the repressor binding to the lac ...
G V, Gursky +5 more
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Binding of lac repressor to the secondary lac operator in Escherichia coli
Molecular and General Genetics MGG, 1978In the lac operon, the existence of a secondary repressor binding site, inside Z gene, had been inferred from in vitro binding studies (Reznikoff et al., 1974; Gilbert et al., 1975). A series of deletions have been constructed from a lac transducing lambda bacteriophage.
A, Rambach, M, Lebastard
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NMR Studies of lac Operator and lac Repressor
2003For many years, and even now, the lac operon of Escherichia coli has been a model for gene regulation. The lac repressor is a tetrameric protein that binds strongly the lac operator and the affinity of which is modulated by an inducer molecule. The lac repressor–lac operator complex was the first nucleic acid–protein complex studied with success by NMR.
Lancelot, Gérard, Paquet, Françoise
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Lac repressor — Lac operator complexes
European Biophysics Journal, 1987Complexes between the Lac repressor and a small DNA operator fragment (29 base pairs) were investigated using polyacrylamide gel electrophoresis and solution X-ray scattering. Titration of the DNA fragment with the repressor, followed by gel electrophoresis showed that only two types of complexes are formed with repressor/operator ratios of 0.5 and 2 ...
F, Culard +3 more
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Journal of Molecular Biology, 2013
The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
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The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
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Biochemistry, 1980
We have shown previously that lac repressor binds specifically and quantitatively to lac operator restriction fragments which have been complexed with histones to form artificial nucleosomes (203 base pair restriction fragment) or core particles (144 base pair restriction fragment.
M V, Chao, H G, Martinson, J D, Gralla
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We have shown previously that lac repressor binds specifically and quantitatively to lac operator restriction fragments which have been complexed with histones to form artificial nucleosomes (203 base pair restriction fragment) or core particles (144 base pair restriction fragment.
M V, Chao, H G, Martinson, J D, Gralla
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Harbouring brood lac: envisioning lac clusters
Development in Practice, 2021openaire +1 more source