DNA supercoiling, a critical signal regulating the basal expression of the lac operon in Escherichia coli. [PDF]
Sci Rep, 2016 Escherichia coli lac repressor (LacI) is a paradigmatic transcriptional factor that controls the expression of lacZYA in the lac operon. This tetrameric protein specifically binds to the O1, O2 and O3 operators of the lac operon and forms a DNA loop to ...
Fulcrand G +6 more
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Network Topology as a Driver of Bistability in the lac Operon [PDF]
Journal of Computational Biology, 2008 The lac operon in Escherichia coli has been studied extensively and is one of the earliest gene systems found to undergo both positive and negative control. The lac operon is known to exhibit bistability, in the sense that the operon is either induced or
Alan Veliz-Cuba +3 more
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Deterministic and stochastic population-level simulations of an artificial
BMC Bioinformatics, 2011 Background The lac operon genetic switch is considered as a paradigm of genetic regulation. This system has a positive feedback loop due to the LacY permease boosting its own production by the facilitated transport of inducer into the cell and the ...
Zygourakis Kyriacos, Stamatakis Michail
doaj +5 more sources
The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition [PDF]
Frontiers in Microbiology, 2021 The lac operon is one of the best known gene regulatory circuits and constitutes a landmark example of how bacteria tune their metabolism to nutritional conditions.
Catarina Pinto +3 more
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The effect of stochasticity on the lac operon: an evolutionary perspective. [PDF]
PLoS Computational Biology, 2007 The role of stochasticity on gene expression is widely discussed. Both potential advantages and disadvantages have been revealed. In some systems, noise in gene expression has been quantified, in among others the lac operon of Escherichia coli.
Milan van Hoek, Paulien Hogeweg
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Diversity in
mBio, 2019 Transcription of bacterial genes is controlled by the coordinated action of cis- and trans-acting regulators. The activity and mode of action of these regulators can reflect different requirements for gene products in different environments.
Kelly N. Phillips +6 more
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Building the lac Operon: A Guided-Inquiry Activity Using 3D-Printed Models [PDF]
Journal of Microbiology & Biology Education, 2020 Undergraduate biology courses rely heavily on visual representation of information. Students view images of plants, animals, and microbes, interpret data presented in graphs, and use drawings to understand how cells and molecules interact in three ...
Claire L. Gordy +4 more
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Acquisition of the lac operon by Salmonella enterica. [PDF]
BMC Microbiol, 2015 Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geographical regions worldwide.
Leonard SR, Lacher DW, Lampel KA.
europepmc +6 more sources
RNA polymerase supply and flux through the lac operon in Escherichia coli. [PDF]
Philos Trans R Soc Lond B Biol Sci, 2016 Chromatin immunoprecipitation, followed by quantification of immunoprecipitated DNA, can be used to measure RNA polymerase binding to any DNA segment in Escherichia coli.
Sendy B, Lee DJ, Busby SJ, Bryant JA.
europepmc +4 more sources
Development of a Lac Operon Concept Inventory (LOCI). [PDF]
CBE Life Sci Educ, 2016 Concept inventories (CIs) are valuable tools for educators that assess student achievement and identify misconceptions held by students. Results of student responses can be used to adjust or develop new instructional methods for a given topic. The regulation of gene expression in both prokaryotes and eukaryotes is an important concept in genetics and ...
Stefanski KM +2 more
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