Network Topology as a Driver of Bistability in the lac Operon [PDF]
J Comput Biol. 2011 Jun;18(6):783-94, 2008 The lac operon in Escherichia coli has been studied extensively and is one of the earliest gene systems found to undergo both positive and negative control. The lac operon is known to exhibit bistability, in the sense that the operon is either induced or uninduced. Many dynamical models have been proposed to capture this phenomenon.
Alan Veliz-Cuba +3 more
arxiv +10 more sources
The effect of stochasticity on the lac operon: an evolutionary perspective. [PDF]
PLoS Computational Biology, 2007 The role of stochasticity on gene expression is widely discussed. Both potential advantages and disadvantages have been revealed. In some systems, noise in gene expression has been quantified, in among others the lac operon of Escherichia coli.
Milan van Hoek, Paulien Hogeweg
doaj +8 more sources
The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition [PDF]
Frontiers in Microbiology, 2021 The lac operon is one of the best known gene regulatory circuits and constitutes a landmark example of how bacteria tune their metabolism to nutritional conditions.
Catarina Pinto +3 more
doaj +6 more sources
DNA supercoiling, a critical signal regulating the basal expression of the lac operon in Escherichia coli. [PDF]
Sci Rep, 2016 Escherichia coli lac repressor (LacI) is a paradigmatic transcriptional factor that controls the expression of lacZYA in the lac operon. This tetrameric protein specifically binds to the O1, O2 and O3 operators of the lac operon and forms a DNA loop to ...
Fulcrand G +6 more
europepmc +5 more sources
Deterministic and stochastic population-level simulations of an artificial
BMC Bioinformatics, 2011 Background The lac operon genetic switch is considered as a paradigm of genetic regulation. This system has a positive feedback loop due to the LacY permease boosting its own production by the facilitated transport of inducer into the cell and the ...
Zygourakis Kyriacos, Stamatakis Michail
doaj +4 more sources
Determinants of bistability in induction of the Escherichia coli lac operon [PDF]
arXiv, 2008 We have developed a mathematical model of regulation of expression of the Escherichia coli lac operon, and have investigated bistability in its steady-state induction behavior in the absence of external glucose. Numerical analysis of equations describing regulation by artificial inducers revealed two natural bistability parameters that can be used to ...
David W. Dreisigmeyer +4 more
arxiv +7 more sources
A modified consumer inkjet for spatiotemporal control of gene expression. [PDF]
PLoS ONE, 2009 This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was
Daniel J Cohen +2 more
doaj +9 more sources
Physiological regulation of a decontrolled lac operon [PDF]
Journal of Bacteriology, 1977 The expression of the lac operon was studied under a variety of growth conditions in induced and in constitutive cells of Escherichia coli that carried different catabolite-insensitive lac promoters. Use of such "decontrolled" lac operons permitted a study of the expression of an operon that was presumably subject only to passive control. Since the use
Barry L. Wanner +2 more
openalex +3 more sources
Adaptive evolution of the lactose utilization network in experimentally evolved populations of Escherichia coli. [PDF]
PLoS Genetics, 2012 Adaptation to novel environments is often associated with changes in gene regulation. Nevertheless, few studies have been able both to identify the genetic basis of changes in regulation and to demonstrate why these changes are beneficial.
Selwyn Quan +6 more
doaj +8 more sources
Diversity in
mBio, 2019 Transcription of bacterial genes is controlled by the coordinated action of cis- and trans-acting regulators. The activity and mode of action of these regulators can reflect different requirements for gene products in different environments.
Kelly N. Phillips +6 more
doaj +2 more sources