Physiological regulation of a decontrolled lac operon [PDF]
Journal of Bacteriology, 1977 The expression of the lac operon was studied under a variety of growth conditions in induced and in constitutive cells of Escherichia coli that carried different catabolite-insensitive lac promoters. Use of such "decontrolled" lac operons permitted a study of the expression of an operon that was presumably subject only to passive control. Since the use
Barry L. Wanner +2 more
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Transient Repression of the lac Operon [PDF]
Journal of Bacteriology, 1967 Severe transient repression of constitutive or induced β-galactosidase synthesis occurs upon the addition of glucose to cells of Escherichia coli growing on glycerol, succinic acid, or lactic acid. Only mutants particularily well adapted to growth on glucose exhibit this phenomenon when transferred to a glucose ...
B Tyler +2 more
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Wrapped-around models for the lac operon complex. [PDF]
Biophys J, 2010 The protein-DNA complex, involved in the lac operon of enteric bacteria, is paradigmatic in understanding the extent of DNA bending and plasticity due to interactions with protein assemblies acting as DNA regulators. For the lac operon, two classes of structures have been proposed: 1), with the protein tetramer lying away from the DNA loop (wrapped ...
La Penna G, Perico A.
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Interplay of protein and DNA structure revealed in simulations of the lac operon. [PDF]
PLoS ONE, 2013 The E. coli Lac repressor is the classic textbook example of a protein that attaches to widely spaced sites along a genome and forces the intervening DNA into a loop.
Luke Czapla +3 more
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Lac Operon Boolean Models: Dynamical Robustness and Alternative Improvements
Mathematics, 2021 In Veliz-Cuba and Stigler 2011, Boolean models were proposed for the lac operon in Escherichia coli capable of reproducing the operon being OFF, ON and bistable for three (low, medium and high) and two (low and high) parameters, representing the ...
Marco Montalva-Medel +3 more
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BMC Microbiology, 2020 Background The msaABCR operon regulates several staphylococcal phenotypes such as biofilm formation, capsule production, protease production, pigmentation, antibiotic resistance, and persister cells formation.
Gyan S. Sahukhal +4 more
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Direct measurements of IPTG enable analysis of the induction behavior of
Microbial Cell Factories, 2012 Background The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production.
Fernández-Castané Alfred +2 more
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Dynamic model of gene regulation for the lac operon [PDF]
Journal of Physics: Conference Series, 2011 Gene regulatory network is a collection of DNA which interact with each other and with other matter in the cell. The lac operon is an example of a relatively simple genetic network and is one of the best-studied structures in the Escherichia coli ...
Asma Ben-Halim +7 more
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Hybrid de novo Genome Assembly of Erwinia sp. E602 and Bioinformatic Analysis Characterized a New Plasmid-Borne lac Operon Under Positive Selection [PDF]
Frontiers in Microbiology, 2021 Our previous study identified a new β-galactosidase in Erwinia sp. E602. To further understand the lactose metabolism in this strain, de novo genome assembly was conducted by using a strategy combining Illumina and PacBio sequencing technology. The whole
Yu Xia +10 more
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Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration. [PDF]
Open Microbiol J, 2015 The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment.
Malakar P.
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