Results 21 to 30 of about 128,431 (240)
Origin of bistability in the lac Operon. [PDF]
Biophys J, 2007 Multistability is an emergent dynamic property that has been invoked to explain multiple coexisting biological states. In this work, we investigate the origin of bistability in the lac operon. To do this, we develop a mathematical model for the regulatory pathway in this system and compare the model predictions with other experimental results in which ...
Santillán M, Mackey MC, Zeron ES.
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The Location of the Repressor Binding Sites in the lac Operon [PDF]
Proceedings of the National Academy of Sciences, 1974 The affinity of various mutant lac DNAs for the lac repressor was examined by means of a competition binding assay. The results indicate that there exist at least two binding sites for the lac repressor.
William S. Reznikoff +2 more
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Structure of the lac Operon Galactoside Acetyltransferase [PDF]
Structure, 2002 The galactoside acetyltransferase (thiogalactoside transacetylase) of Escherichia coli (GAT, LacA, EC 2.3.1.18) is a gene product of the classical lac operon. GAT may assist cellular detoxification by acetylating nonmetabolizable pyranosides, thereby preventing their reentry into the cell.
Xingguo Wang +2 more
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From Binary to Multivalued to Continuous Models: The lac Operon as a Case Study
Journal of Integrative Bioinformatics, 2010 Using the lac operon as a paradigmatic example for a gene regulatory system in prokaryotes, we demonstrate how qualitative knowledge can be initially captured using simple discrete (Boolean) models and then stepwise refined to multivalued logical models ...
Franke Raimo +2 more
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Bistable behavior of the lac operon in E. coli when induced with a mixture of lactose and TMG
Frontiers in Physiology, 2010 In this work we investigate multistability in the lac operon of Escherichia coli when it is induced by a mixture of lactose and the non-metabolizable thiomethyl galactoside (TMG).
Orlando Díaz-Hernández +3 more
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Understanding operon architecture using LEGO bricks [PDF]
Journal of Microbiology & Biology EducationMany undergraduates struggle to interpret abstract concepts in molecular biology. Modeling can facilitate learning by making these abstract concepts tangible.
Dena R. Hammond-Weinberger +1 more
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Historical Contingency Causes Divergence in Adaptive Expression of the lac Operon. [PDF]
Mol Biol Evol, 2021 Karkare K +3 more
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Transcription of the lac Operon of Escherichia coli
Journal of Biological Chemistry, 1974 The effects of induction and of transient and catabolite repression on lac-specific RNA in cells of Escherichia coli were investigated. The rate of lac-specific RNA synthesis increases linearly for 3 min after induction and then becomes constant. The accumulation of RNA complementary to the promoter proximal lac Z gene begins before accumulation of RNA
Terrance Cooper, Boris Magasanik
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Catabolite repression of the lac operon. Repression of translation [PDF]
Biochemical Journal, 1969 1. Experiments were devised to show whether catabolite repression of β-galactosidase synthesis operates at the level of transcription or of translation. Escherichia coli K12 was induced for a short period in non-repressing medium (glycerol–minimal medium), and transcription of the lac operon was terminated by either of two methods; glucose was then ...
Michael D. Yudkin, V. Moses
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The three operators of the lac operon cooperate in repression [PDF]
The EMBO Journal, 1990 We tested the effect of systematic destruction of all three lac operators of the chromosomal lac operon of Escherichia coli on repression by Lac repressor. Absence of just one 'pseudo-operator' O2 or O3 decreases repression by wild-type tetrameric Lac repressor approximately 2- to 3-fold; absence of both 'pseudo-operators' decreases repression greater ...
Stefan Oehler +3 more
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