Structure of the lac Operon Galactoside Acetyltransferase [PDF]
Structure, 2002 The galactoside acetyltransferase (thiogalactoside transacetylase) of Escherichia coli (GAT, LacA, EC 2.3.1.18) is a gene product of the classical lac operon. GAT may assist cellular detoxification by acetylating nonmetabolizable pyranosides, thereby preventing their reentry into the cell.
Xingguo Wang +2 more
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Concentration and Length Dependence of DNA Looping in Transcriptional Regulation [PDF]
, 2008 In many cases, transcriptional regulation involves the binding of transcription factors at sites on the DNA that are not immediately adjacent to the promoter of interest. This action at a distance is often mediated by the formation of DNA loops: Binding at two or more sites on the DNA results in the formation of a loop, which can bring the ...
Beausang, John F. +6 more
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Interplay of protein and DNA structure revealed in simulations of the lac operon. [PDF]
PLoS ONE, 2013 The E. coli Lac repressor is the classic textbook example of a protein that attaches to widely spaced sites along a genome and forces the intervening DNA into a loop.
Luke Czapla +3 more
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Historical Contingency Causes Divergence in Adaptive Expression of the lac Operon. [PDF]
Mol Biol Evol, 2021 Karkare K +3 more
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Transcription of the lac Operon of Escherichia coli
Journal of Biological Chemistry, 1974 The effects of induction and of transient and catabolite repression on lac-specific RNA in cells of Escherichia coli were investigated. The rate of lac-specific RNA synthesis increases linearly for 3 min after induction and then becomes constant. The accumulation of RNA complementary to the promoter proximal lac Z gene begins before accumulation of RNA
Terrance Cooper, Boris Magasanik
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Catabolite repression of the lac operon. Repression of translation [PDF]
Biochemical Journal, 1969 1. Experiments were devised to show whether catabolite repression of β-galactosidase synthesis operates at the level of transcription or of translation. Escherichia coli K12 was induced for a short period in non-repressing medium (glycerol–minimal medium), and transcription of the lac operon was terminated by either of two methods; glucose was then ...
Michael D. Yudkin, V. Moses
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A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli [PDF]
Scientific ReportsProtein production using Escherichia coli is a cornerstone of modern biotechnology. In this study, we developed a novel auto-expression medium to maximize protein production. Each E.
James Bosco +23 more
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Catabolite repression of the lac operon. Effect of mutations in the lac promoter [PDF]
Biochemical Journal, 1970 1. Several lac diploid strains of Escherichia coli were constructed and tested to discover whether mutations in the lac promoter alleviate catabolite repression. 2. In each of these diploids the chromosome carries one of the promoter mutations, L8, L29 or L1; so that the rate of synthesis of the enzymes of the lac operon is only 2–6% of the fully ...
Michael D. Yudkin
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The three operators of the lac operon cooperate in repression [PDF]
The EMBO Journal, 1990 We tested the effect of systematic destruction of all three lac operators of the chromosomal lac operon of Escherichia coli on repression by Lac repressor. Absence of just one 'pseudo-operator' O2 or O3 decreases repression by wild-type tetrameric Lac repressor approximately 2- to 3-fold; absence of both 'pseudo-operators' decreases repression greater ...
Stefan Oehler +3 more
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RNA polymerase supply and flux through the lac operon in Escherichia coli. [PDF]
Philos Trans R Soc Lond B Biol Sci, 2016 Sendy B, Lee DJ, Busby SJ, Bryant JA.
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