Results 41 to 50 of about 41,627 (225)

Mechanoregulatory Effects of Cell‐Scale Microwells on Epithelial Cell Phenotype

Advanced Functional Materials, EarlyView.
In small polycaprolactone microwells, A549 epithelial cells span well edges, in contrast to cells growing on flat substrates. Focal adhesion sites (yellow) concentrate at topographic boundaries, while cytoskeletal tension (magenta stress fibers) is transmitted to the nucleus (blue), reducing nuclear sphericity.
Ruiwen He, Wang Sik Lee, Phattadon Yajan, Aaron Lee, Mauro Sousa de Almeida, Sandeep Keshavan, Jozef Adamcik, Céline Loussert‐Fonta, Dimitri Vanhecke, Barbara Rothen‐Rutishauser, Alke Petri‐Fink   +10 more
wiley   +1 more source

Statins and Histone Deacetylase Inhibitors Affect Lamin A/C – Histone Deacetylase 2 Interaction in Human Cells

Frontiers in Cell and Developmental Biology, 2019
We recently identified lamin A/C as a docking molecule for human histone deacetylase 2 (HDAC2) and showed involvement of HDAC2-lamin A/C complexes in the DNA damage response.
Elisabetta Mattioli, Elisabetta Mattioli, Davide Andrenacci, Davide Andrenacci, Antonello Mai, Sergio Valente, Joke Robijns, Winnok H. De Vos, Cristina Capanni, Cristina Capanni, Giovanna Lattanzi, Giovanna Lattanzi   +11 more
doaj   +1 more source

The Floor‐Ceiling‐Chip, or 2 × 2D = Pseudo‐3D—Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell‐Adhesive Protein Micropatterns

Advanced Healthcare Materials, EarlyView.
Here, we present a novel 3D cell patterning and culture platform. The “Floor‐Ceiling‐Chip” (FC‐Chip) consists of two opposing track‐etched membranes, creating a pseudo‐3D microenvironment for the cells in between. By providing the membranes with micropatterned cell‐adhesive islands of varying geometries and sizes, the FC‐Chip enables control over cell ...
Urandelger Tuvshindorj, Francesca Giacomini, Esra Güben Kaçmaz, Tim ten Brink, Lorenzo Moroni, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, Jan de Boer, Roman Truckenmüller   +10 more
wiley   +1 more source

Common Signatures of Altered Gene Regulation and Invasiveness of Different Breast Cancer Cell Lines after Matrix Interface Crossing

Advanced Healthcare Materials, EarlyView.
Interface transmigration reprograms triple‐negative breast cancer cells, triggering a shared switch toward more aggressive and invasive phenotypes. Using a collagen I interface model, this study identifies shared transcriptional changes involving proliferation, chromatin remodeling, and DNA repair pathways.
Cornelia Clemens, Hannah Trampert, Nataliia Kotsiuba, Tilo Pompe   +3 more
wiley   +1 more source

Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.

PLoS ONE, 2017
The type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2.
Elise Kaspi, Diane Frankel, Julien Guinde, Sophie Perrin, Sophie Laroumagne, Andrée Robaglia-Schlupp, Kevin Ostacolo, Karim Harhouri, Rachid Tazi-Mezalek, Joelle Micallef, Hervé Dutau, Pascale Tomasini, Annachiara De Sandre-Giovannoli, Nicolas Lévy, Pierre Cau, Philippe Astoul, Patrice Roll   +16 more
doaj   +1 more source

Tendon Organoids Enable Functional Tendon Rejuvenation Through ALKBH5‐Dependent RNA Demethylation

Advanced Science, EarlyView.
FT organoids reverse the aged phenotype of tendon cells, reinstating a fetal‐like state. This breakthrough establishes a potent cell source for tendon tissue engineering, effectively advancing regenerative medicine. ABSTRACT Adult tendon injuries pose a major clinical challenge due to limited self‐repair capacity, resulting in suboptimal regeneration ...
Tian Qin, Aini Pan, Zhuoning Miao, Yanyan Zhao, Xianan Mo, Heng Sun, Chunmei Fan, Junyu Guo, Bingbing Wu, Weiliang Shen, Qiangqiang Zheng, Jun Lu, Xi Jiang, Zi Yin, Xiao Chen   +14 more
wiley   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Baculovirus infection induces disruption of the nuclear lamina

Scientific Reports, 2017
Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during
Xiaomei Zhang, Kaiyan Xu, Denghui Wei, Wenbi Wu, Kai Yang, Meijin Yuan   +5 more
doaj   +1 more source

Revisiting the truncated lamin A produced by a commonly used strain of Lmna knockout mice

Nucleus, 2023
The Lmna knockout mouse (Lmna–/–) created by Sullivan and coworkers in 1999 has been widely used to examine lamin A/C function. The knockout allele contains a deletion of Lmna intron 7–exon 11 sequences and was reported to be a null allele.
Joonyoung R. Kim, Paul H. Kim, Ashley Presnell, Yiping Tu, Stephen G. Young   +4 more
doaj   +1 more source

Lamin-A Mechano-Protects the Heart [PDF]

Developmental Cell, 2019
In this issue of Developmental Cell, Cho et al. (2019) find that lamin-A levels in the nuclear envelope are regulated in response to mechanical stimuli to prevent the nucleus from rupture, keep DNA repair factors in the nucleus, and consequentially "mechano-protect the genome."
Ioannis, Xanthis, Thomas, Iskratsch
openaire   +2 more sources