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Amine production byLeishmania mexicana
Annals of Tropical Medicine & Parasitology, 1985Growing promastigotes of Leishmania mexicana mexicana excreted large amounts of urea and ammonia into the culture medium. Both promastigotes and amastigotes in short-term, high-density cultures also produced urea and ammonia; the excretion rate was higher in promastigotes.
G H, Coombs, B E, Sanderson
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Heme binding to Leishmania mexicana amazonensis
Molecular and Biochemical Parasitology, 1988Leishmania mexicana amazonensis is a pathogenic parasite whose growth, due to a biosynthetic deficiency, is dependent on a supply of exogenous heme. Utilizing [55Fe]hemin, we have demonstrated that heme binding to non-dividing cultured promastigotes of L. m.
R A, Galbraith, M J, McElrath
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Leishmania mexicana mexicana gp63 is a site-specific neutral endopeptidase
Molecular and Biochemical Parasitology, 1990Leishmania mexicana, like other species of the genus, has a major 63-kDa surface glycoprotein (gp63) that is an active protease. Reports differ as to whether gp63 is a neutral or an acidic protease. Using three radiolabeled synthetic peptide substrates, gp63 purified from L. m.
H S, Ip +3 more
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Molecular and Biochemical Parasitology, 1994
The glycolytic enzyme glucosephosphate isomerase (PGI) is present in two different cell compartments of Leishmania mexicana promastigotes; more than 90% of the activity was detected in the cytosol, the remainder in glycosomes. This subcellular distribution contrasts with that in Trypanosoma brucei, in which the enzyme activity has been mainly located ...
K, Nyame +3 more
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The glycolytic enzyme glucosephosphate isomerase (PGI) is present in two different cell compartments of Leishmania mexicana promastigotes; more than 90% of the activity was detected in the cytosol, the remainder in glycosomes. This subcellular distribution contrasts with that in Trypanosoma brucei, in which the enzyme activity has been mainly located ...
K, Nyame +3 more
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Purine phosphoribosyltransferases of Leishmania mexicana mexicana and other flagellate protozoa
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1985Amastigotes and cultured promastigotes of Leishmania mexicana mexicana and L. m. amazonensis, cultured promastigotes of L. donovani and L. tarentolae, and the culture forms of Crithidia fasciculata, Herpetomonas muscarum muscarum and H. m. ingenoplastis all possessed four phosphoribosyltransferase (PRTase) activities: adenine PRTase, hypoxanthine ...
H F, Hassan, G H, Coombs
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Molecular and Biochemical Parasitology, 1990
The multiple cysteine proteinases characteristic of the amastigote forms of Leishmania mexicana mexicana have been shown to be of three types. The groups of enzymes are distinguished by their substrate specificities and physical properties and have been purified free from other proteinases and most other proteins.
C D, Robertson, G H, Coombs
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The multiple cysteine proteinases characteristic of the amastigote forms of Leishmania mexicana mexicana have been shown to be of three types. The groups of enzymes are distinguished by their substrate specificities and physical properties and have been purified free from other proteinases and most other proteins.
C D, Robertson, G H, Coombs
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Leishmania mexicana: Energy metabolism of amastigotes and promastigotes
Experimental Parasitology, 1982Abstract The utilisation of substrates by Leishmania mexicana amastigotes and promastigotes differed significantly. The rates of uptake and catabolism of nonesterified fatty acids were up to 10-fold higher with amastigotes. Almost all the available exogenous fatty acids were consumed during amastigote transformation and by stationary phase of ...
D T, Hart, G H, Coombs
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ANTIPROLIFERATIVE EFFECT OF ILLIMAQUINONE ON LEISHMANIA MEXICANA
Cell Biology International, 1997Illimaquinone, a sponge metabolite that disrupts the Golgi complex in mammalian cells, stopped proliferation and induced morphological and ultrastructural changes in promastigotes of L. mexicana Radioactive labeling of proteins demonstrates an increased excretion function and diminution of membrane acid phosphatase activity, due probably to the ...
H R, Rangel, F, Dagger, R S, Compagnone
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Leishmania mexicana: Molecular cloning and characterization of enolase
Experimental Parasitology, 2007The gene of Leishmania mexicana enolase was cloned and overexpressed in Escherichia coli as an active enzyme; the protein was biochemically analyzed. This enolase shares with enolases from other trypanosomatids the presence of three atypical residues, each with a reactive side group, near the active site, already described for the enzyme from ...
Wilfredo, Quiñones +6 more
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Inducible protein stabilization system in Leishmania mexicana
Molecular and Biochemical Parasitology, 2017Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types.
Lucie, Podešvová +2 more
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