Results 141 to 150 of about 116,783 (314)

The RLR intrinsic antiviral system is expressed in neural retina and restricts lentiviral transduction of human Mueller cells. [PDF]

Exp Eye Res, 2023
Sauter MM, Noel H, Brandt CR.
europepmc   +1 more source

ACSL5 Regulates Glucose Metabolism and Chemotherapy Sensitivity in Colorectal Cancer Cells under Glutamine Deficiency

Advanced Science, EarlyView.
Glutamine deprivation triggers ACSL5 upregulation in tumor cells, sustaining their viability via dual metabolic rewiring programs. ACSL5 enhances glycolysis by relieving p53's inhibition of PGAM1 while also sustaining mitochondrial respiration and TCA cycle flux through promoting IDH2 dimerization.
Shuai Tian, Qiaoxia Zhang, Xuedan Sun, Rick Francis Thorne, Zeyuan Shi, Qiang Ji, Zhangran Sun, Yuanxiang Lu, Qun Zhao, Xianjun Yu, Wanglai Hu, Mian Wu   +11 more
wiley   +1 more source

Improving Lentiviral Transduction of Human Adipose-Derived Mesenchymal Stem Cells. [PDF]

Hum Gene Ther, 2022
Collon K, Gallo MC, Bell JA, Chang SW, Rodman JCS, Sugiyama O, Kohn DB, Lieberman JR.   +7 more
europepmc   +1 more source

The Disordered Region of ASXL1 Acts as an Auto‐Regulator Through Condensation

Advanced Science, EarlyView.
ASXL1's long IDR encodes an electrostatic “basic platform + acidic brake” that autoregulates condensation. Truncation at a clinical hotspot lifts this brake, forming condensates that retarget BRD2, remodel local chromatin accessibility, and impair neutrophil maturation.
Xiao Fang, Qiwei Li, Wenqing Zhang
wiley   +1 more source

A Biomarker‐Driven Ovary–Endometrium Organ‐on‐a‐Chip Mimicking 3D Multicellular Complexity and Menstrual Cyclicity for Predicting Reproductive Toxicity

Advanced Science, EarlyView.
We present a dual‐organ, biomarker‐integrated ovaryendometrium organ‐on‐a‐chip that recapitulates 3D tissue complexity, menstrual cycle dynamics, and hormonal crosstalk. This platform enables real‐time, cell‐typespecific fluorescent readouts of reproductive toxicity using ANGPTL4 and SERPINB2 as early‐response reporters.
Soo‐Rim Kim, Eun‐kyung Min, Choon‐Mi Lee, Chan Hum Park, Byung‐Chul Oh, YunJae Jung, In‐Sun Hong   +6 more
wiley   +1 more source

Designing and Constructing the Lentiviral Vector Coding miRNA-499a and Transduction of Human Mesenchymal Stem Cells

Journal of Mazandaran University of Medical Sciences, 2018
Background and purpose: Lentiviral vectors (LVs) are suitable candidates for gene delivery to cells with stable and high-level of transgene expression in target cells.
Vajiheh Neshati, Samaneh Mollazadeh, Bibi Sedigheh Fazly Bazzaz, Majid Mojarrad, Zeinab Neshati, Mohammad Amin Kerachian   +5 more
doaj  

Cellular Proteo-Transcriptomic Changes in the Immediate Early-Phase of Lentiviral Transduction. [PDF]

Microorganisms, 2021
Linkner TR, Ambrus V, Kunkli B, Szojka ZI, Kalló G, Csősz É, Kumar A, Emri M, Tőzsér J, Mahdi M.   +9 more
europepmc   +1 more source

Development of Endogenous Protein Probes for Characterizing Surface Proteins and Cellular Interactors of Extracellular Vesicles

Advanced Science, EarlyView.
The proximity labeling enzyme APEX2 is displayed on extracellular vesicle (EV) surfaces via genetic fusion with EV‐sorting scaffolds, enabling in situ biotinylation of native surface proteins, adsorbed corona components, and interacting cellular proteins.
Wenyi Zheng, Metoboroghene Mowoe, Wenqing Hou, Daniel W. Hagey, Koshi Imami, Samir EL Andaloussi   +5 more
wiley   +1 more source

Aberrant SUMOylation Restricts the Targetable Cancer Immunopeptidome

Advanced Science, EarlyView.
Pharmacological SUMOylation inhibition (SUMOi) counteracts tumor immune evasion by unmasking an immunogenic HLA‐I peptide and neoepitope repertoire. By restoring HLA‐I ligand availability through increased antigen processing and presentation, enhanced proteasomal cleavage, and modulated TAP1 peptide affinity, SUMOi boosts tumor immunogenicity ...
Uta M. Demel, Anna Meurer, Badeel Kh. Q. Zaghla, Bilge Atay, Daniel Steiert, Luca V. Hummel, Konstandina Isaakidis, Chuanbing Zang, Michael Korenkov, Marlon Schielin, Schayan Yousefian, Shima Mecklenbräuker, Marieluise Kirchner, Simon Haas, Antonia Busse, Philipp Mertins, Stefan Müller, Matthias Wirth, Martin G. Klatt, Ulrich Keller   +19 more
wiley   +1 more source

Lentiviral transduction facilitates RNA interference in the nematode parasite Nippostrongylus brasiliensis. [PDF]

PLoS Pathog, 2021
Hagen J, Sarkies P, Selkirk ME.
europepmc   +1 more source