Results 81 to 90 of about 115,363 (220)

The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference

Retrovirology, 2006
Background RNA interference (RNAi) has proven to be a powerful tool to suppress gene expression and can be used as a therapeutic strategy against human pathogenic viruses such as human immunodeficiency virus type 1 (HIV-1).
Berkhout Ben, ter Brake Olivier, Westerhout Ellen M   +2 more
doaj   +1 more source

Cell-based gene therapy for mending infarcted hearts [PDF]

, 2011
The goal of this study was to analyse the efficiency of a combinatorial cell/growth factor therapy to improve function of infarcted murine hearts. The Insulin-like Growth Factor-1 (IGF-1) isoform, IGF-1Ea, has been shown to reduce scar formation and ...
Poudel, Bhawana, Poudel, Bhawana
core   +1 more source

A Distinct Subset of Highly Proliferative and Lentiviral Vector (LV)-Transducible NK Cells Define a Readily Engineered Subset for Adoptive Cellular Therapy

Frontiers in Immunology, 2019
Genetic engineering is an important tool for redirecting the function of various types of immune cells and their use for therapeutic purpose. Although NK cells have many beneficial therapeutic features, genetic engineering of immune cells for targeted ...
Rafijul Bari, Markus Granzin, Kam Sze Tsang, Andre Roy, Winfried Krueger, Rimas Orentas, Dina Schneider, Rita Pfeifer, Nina Moeker, Els Verhoeyen, Els Verhoeyen, Boro Dropulic, Wing Leung   +12 more
doaj   +1 more source

An Improved Protocol for the Production of Lentiviral Vectors

STAR Protocols, 2020
Summary: Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism.
Logan Y. Brown, Wendy Dong, Boris Kantor
doaj   +1 more source

Targeting lentiviral vectors to antigen-specific immunoglobulins [PDF]

, 2008
Gene transfer into B cells by lentivectors can provide an alternative approach to managing B lymphocyte malignancies and autoreactive B cell-mediated autoimmune diseases.
Black M.E., David Baltimore, Davis T.A., Drake J.R., Dull T., Freeman S.M., Haiguang Yang, Kye il Joo, Leslie Ziegler, Lili Yang, Maloney D.G., Pin Wang, Rankin E.M., Reff M.E., Stripecke R., Vuist W.M., Wierda W.G., Yu J.H.   +17 more
core   +2 more sources

Lentiviral vectors: optimization of packaging, transduction and gene expression [PDF]

The Journal of Gene Medicine, 2004
Gene transfer vectors based on retroviruses including oncogenic retroviruses and lentiviruses provide effective means for the delivery, integration and expression of exogenous genes in mammalian cells. Lentiviral (LV) vectors provide attractive gene delivery vehicles in the context of non-dividing cells.
openaire   +2 more sources

Production of Lentiviral Vector Expressing MicroRNA-148b

مجله دانشکده پزشکی اصفهان, 2017
Background: Micro (mi)RNAs are non-coding endogenous RNAs which regulate gene expression by hybridization to specific binding sites in target mRNA sequences.
Samaneh Mollazadeh, Vajiheh Neshati, Bibi Sedigheh Fazly Bazzaz, Majid Mojarrad, Mohammad Amin Kerachian   +4 more
doaj  

Serum-free Erythroid Differentiation for Efficient Genetic Modification and High-Level Adult Hemoglobin Production

Molecular Therapy: Methods & Clinical Development, 2018
In vitro erythroid differentiation from primary human cells is valuable to develop genetic strategies for hemoglobin disorders. However, current erythroid differentiation methods are encumbered by modest transduction rates and high baseline fetal ...
Naoya Uchida, Selami Demirci, Juan J. Haro-Mora, Atsushi Fujita, Lydia N. Raines, Matthew M. Hsieh, John F. Tisdale   +6 more
doaj   +1 more source

Analysis of Hepatic Lentiviral Vector Transduction: Implications for Preclinical Studies and Clinical Gene Therapy Protocols

Viruses
Lentiviral vector-transduced T cells were approved by the FDA as gene therapy anti-cancer medications. Little is known about the effects of host genetic variation on the safety and efficacy of the lentiviral vector gene delivery system.
Peirong Hu, Yajing Hao, Wei Tang, Graham H. Diering, Fei Zou, Tal Kafri   +5 more
doaj   +1 more source

Human Cytomegalovirus Encoded miR-US25-1-5p Attenuates CD147/EMMPRIN-Mediated Early Antiviral Response. [PDF]

, 2017
Cellular receptor-mediated signaling pathways play critical roles during the initial immune response to Human Cytomegalovirus (HCMV) infection.
Chen, Huizi, Chen, Jun, Chen, Zhinan, Li, Fanni, LIU, Fenyong, Xia, Sisi, Yang, Xiangmin   +6 more
core   +2 more sources