Results 121 to 130 of about 166,720 (266)

BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow‐Transit Constipation

Advanced Science, EarlyView.
Slow‐transit constipation (STC) is a disabling motility disorder with unclear smooth‐muscle mechanisms. Spatial proteomic analysis of STC patient colon reveals both the central pathogenic role of smooth muscle cells (SMCs) in STC and novel regulators of intestinal motility, BIN1 and ALDH1B1.
Jianbo Liu, Hao Zhang, Wenhao Qiao, Ran Liu, Jie Sun, Xiaopei Li, Qin Li, Dongbo Zhao, Dawei Chen, Jingxin Li, Shuxiao Dong   +10 more
wiley   +1 more source

Sheep infection by caprine lentivirus

, 2018
SUMMARY The objective of this study was to demonstrate iatrogenic transmission of small ruminant lentivirus (SRLV) from goats to sheep and horizontal transmission between sheep.
Thiago Sampaio de Souza (4826937), Raymundo Rizaldo Pinheiro (4826943), Dalva Alana Aragão de Azevedo (5833607), Carla Caroline Valença de Lima (5763704), Vanderlan Warlington Souza dos Santos (4826940), Roberta Lomonte Lemos de Brito (4826928), Ronaldo Pereira Dias (4826931), Alice Andrioli (4826952), Joselito Nunes Costa (5694467)   +8 more
core   +1 more source

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine
Lentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for ...
Eduardo Barbieri, Gina N. Mollica, Sobhana A. Sripada, Shrirarjun Shastry, Yuxuan Wu, Arianna Minzoni, Will Smith, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Michael S. Crapanzano, Oliver Rammo, Michael M. Schulte, Michael A. Daniele, Stefano Menegatti   +14 more
doaj   +1 more source

Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

Microbiology Spectrum
Feline infectious peritonitis virus (FIPV), a highly pathogenic subtype of feline coronavirus, is characterized by antigenic variability and immune evasion, resulting in limited efficacy of existing clinical therapeutic regimens.
Yi Zhong, Zhiwei Sun, Ziyan Song, Jinman Ding, Yanwen Song, Yi Li, Guisong Liao, Xin Wang, Yan Zeng, Nan Hu, Xingcui Zhang, Zhenhui Song   +11 more
doaj   +1 more source

Epigenetic Silencing of RFX7 Defines a Transcriptional Axis Linking Lactate Metabolism to Immune Checkpoint Therapy in Glioblastoma

Advanced Science, EarlyView.
Glioblastoma (GBM) exhibits RFX7 silencing via promoter hypermethylation, which downregulates PIK3IP1 to activate PI3K/AKT signaling, increase lactate accumulation, and enhance histone H4K12 lactylation. This lactylation upregulates PD‐L1/CSF1 expression and drives the immunosuppressive microenvironment formation.
Liying Han, Jinpeng Zhou, Gang Zhu, Fan Chen, Leiyang Li, Wenxing Cui, Fang Sun, Tian Feng, Yue Zhang, Qiang Wang, Shuoyao Ma, Chengxuan Guo, Ziwen Zhang, Kai Wang, Jiahui Wang, Liangbo Wang, Wencong Li, Chenyi Yuan, Haixiao Liu, Liang Wang, Yan Qu   +20 more
wiley   +1 more source

Knockdown of FAM81A with lentivirus shRNA.

(A) Primary cultured mouse cortical neurons were infected with lentivirus encoding FAM81A shRNAs at DIV4. At DIV16, neurons were harvested to extract mRNA. After the preparation of cDNA, real-time PCR was performed.
Taisei Hirouchi (18121663), Toru Takumi (72606), Takeo Saneyoshi (701663), Mark O. Collins (10944312), Toshihiko Shirafuji (847544), Takeshi Kaizuka (775489), Seth G. N. Grant (11820940), Yasunori Hayashi (701664)   +7 more
core   +1 more source

Multi‐Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean‐Type and Chinese Indigenous Pigs

Advanced Science, EarlyView.
Multi‐omics analyses uncover breed‐specific cis‐regulatory landscapes and higher‐order chromatin architectural differences that underlie early postnatal muscle fiber divergence in pigs. A super‐enhancer upstream of PPP3CB recruits MEF2C to activate PPP3CB transcription, while the PPP3CB–MEF2C positive feedback loop promotes oxidative muscle fiber ...
Shuailong Zheng, Hainan Wu, Min Liu, Kunpeng Wu, Shuntao Huang, Zulfiqar Ahmed, Lenox Pius, Mengjin Zhu, Dequan Xu   +8 more
wiley   +1 more source

Electrophysiological analysis of lentivirus-infected neurons.

, 2013
EGFP-expressing lentivirus vectors were stereotaxically injected into the CA1 pyramidal neurons, and patch clamp analyses were carried out on infected and non-infected neurons. A. B.
Yoshihiko Wakazono (497924), Kogo Takamiya (123337), Takato Kunitake (497923), Jyoji Tsutajima (497922)   +3 more
core   +1 more source

DTX3L Inhibits the EMT, Metastasis, and Stem‐Like Features of Gastric Cancer Through Promoting GSK‐3β Dependent SNAI1 Decay

Advanced Science, EarlyView.
This research identifies DTX3L as a critical tumor suppressor that inhibits epithelial‐mesenchymal transition (EMT), invasion and metastasis in gastric cancer. By functioning as an E3 ubiquitin ligase, DTX3L targets the master EMT regulator SNAI1 for GSK‐3β‐dependent proteasomal degradation.
Yang Chen, Zhen Li, Jiajia Shen, Jingyu Lin, Xiaoli Zhao, Rui Zhang, Ying Han, Zhen Wang   +7 more
wiley   +1 more source

Targeted depletion of BMI-1 through lentivirus-mediated siRNA.

, 2013
(A) Representetive graphs of SAOS-2 cells infected with indicated lentivirus at MOI of 10 were shown (×400). Following infection of cells with indicated lentivirus for 5 days, BMI-1 mRNA levels were measured with real-time PCR (B), and protein levels ...
Zhihong Wu (224503), Guixing Qiu (224508), Dongsheng Hao (356610), Yuanhui Duan (356612), Li Min (356608), Dafu Chen (291913), Yipeng Wang (9800)   +6 more
core   +1 more source