Results 31 to 40 of about 172,662 (343)

Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing

Nucleic Acids Research, 2019
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system discovered using bacteria has been repurposed for genome editing in human cells. Transient expression of the editor proteins (e.g.
B. Lu, Parisa Javidi-Parsijani, Vishruti Makani, F. Mehraein‐Ghomi, W. Sarhan, Dongjun Sun, K. Yoo, Zachary Atala, Pin Lyu, A. Atala   +9 more
semanticscholar   +1 more source

Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons

eLife, 2019
Direct conversion of human somatic fibroblasts into induced neurons (iNs) allows for the generation of functional neurons while bypassing any stem cell intermediary stages.
Joseph Herdy, Simon Schafer, Yongsung Kim, Zoya Ansari, Dina Zangwill, Manching Ku, Apua Paquola, Hyungjun Lee, Jerome Mertens, Fred H Gage   +9 more
doaj   +1 more source

Beyond retrovirus infection: HIV meets gene therapy

Genetics and Molecular Biology, 2006
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA.
Flávia Helena da Silva, Tiago Pires Dalberto, Nance Beyer Nardi   +2 more
doaj   +1 more source

Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

bioRxiv, 2019
Background Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation.
R. Gratacap, Tim Regan, C. Dehler, S. Martin, P. Boudinot, B. Collet, R. Houston   +6 more
semanticscholar   +1 more source

Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits

Frontiers in Neural Circuits, 2018
Retrograde tracing is a key facet of neuroanatomical studies involving long distance projection neurons. Previous groups have utilized a variety of tools ranging from classical chemical tracers to newer methods employing viruses for gene delivery.
Imran S. Sheikh, Kathleen M. Keefe, Noelle A. Sterling, Ian P. Junker, Chidubem I. Eneanya, Yingpeng Liu, Xiao-Qing Tang, George M. Smith   +7 more
doaj   +1 more source

Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins

Bioengineering, 2022
Lentiviral vectors are unique and highly efficient genetic tools to incorporate genetic materials into the genome of a variety of cells whilst conserving biosafety.
Seçkin Akgül, Carolin Offenhäuser, Anja Kordowski, Bryan W. Day   +3 more
doaj   +1 more source

Retroviral Integration Site Selection

Viruses, 2010
The stable insertion of a copy of their genome into the host cell genome is an essential step of the life cycle of retroviruses. The site of viral DNA integration, mediated by the viral-encoded integrase enzyme, has important consequences for both the ...
Angela Ciuffi, Sébastien Desfarges
doaj   +1 more source

Manufacture of Third-Generation Lentivirus for Preclinical Use, with Process Development Considerations for Translation to Good Manufacturing Practice

Human Gene Therapy Methods, 2017
Lentiviral vectors are used in laboratories around the world for in vivo and ex vivo delivery of gene therapies, and increasingly clinical investigation as well as preclinical applications.
C. Gándara, Valerie S. Affleck, Elizabeth A. Stoll   +2 more
semanticscholar   +1 more source

Toward Personalized Gene Therapy: Characterizing the Host Genetic Control of Lentiviral-Vector-Mediated Hepatic Gene Delivery

Molecular Therapy: Methods & Clinical Development, 2017
The success of lentiviral vectors in curing fatal genetic and acquired diseases has opened a new era in human gene therapy. However, variability in the efficacy and safety of this therapeutic approach has been reported in human patients.
Thipparat Suwanmanee, Martin T. Ferris, Peirong Hu, Tong Gui, Stephanie A. Montgomery, Fernando Pardo-Manuel de Villena, Tal Kafri   +6 more
doaj   +1 more source

Exploration of high-efficiency transfection methods for sheep fibroblasts OAR-L1

浙江大学学报. 农业与生命科学版, 2022
In order to achieve high-efficiency expression of exogenous protein in sheep lung fibroblasts OAR-L1, and to explore a suitable transfection method for the cell line, the transfection efficiencies of polyethyleneimine (PEI), LipofectamineTM 2000 ...
WU Fei, WU Jie, CHEN Xueqiu, ZHOU Jingru, ZHANG Hui, HUANG Yan, SHI Hengzhi, YANG Yi, MA Guangxu, DU Aifang   +9 more
doaj   +1 more source