Results 61 to 70 of about 104,318 (277)

High-resolution 3D optical microscopy inside the beating zebrafish heart using prospective optical gating [PDF]

open access: yes, 2012
3D fluorescence imaging is a fundamental tool in the study of functional and developmental biology, but effective imaging is particularly difficult in moving structures such as the beating heart.
Girkin, J.M., Love, G.D., Taylor, J.M.
core   +1 more source

Light Sheet Fluorescence Microscopy Image Acquisition v1 [PDF]

open access: yes, 2020
The Zeiss Lightsheet 7 is used to quickly and efficiently scan large tissue volumes using laser light sheet illumination. The focused laser sheets and imaging plane are static while the tissue is moved throughout the selected volume for three dimensional image acquisition.
Seth Currlin   +2 more
openaire   +1 more source

Meeting report: First light sheet based fluorescence microscopy workshop [PDF]

open access: yesBiotechnology Journal, 2010
In Jena, at the turn of the century, Richard Zsig-mondy (1865–1929), working together with thephysicist Heinrich Siedentopf, had the brilliantidea to use a light sheet to illuminate from the sidethe colloid solution they were investigating [1].From there the idea of light sheet illumination van-ished from the scientific spotlight until it was re-vived ...
Reynaud, E., Tomancák, P.
openaire   +2 more sources

Quantum Emitters in Hexagonal Boron Nitride: Principles, Engineering and Applications

open access: yesAdvanced Functional Materials, EarlyView.
Quantum emitters in hexagonal boron nitride have emerged as a promising candidate for quantum information science. This review examines the fundamentals of these quantum emitters, including their level structures, defect engineering, and their possible chemical structures.
Thi Ngoc Anh Mai   +8 more
wiley   +1 more source

Single-molecule study for a graphene-based nano-position sensor [PDF]

open access: yes, 2014
In this study we lay the groundwork for a graphene-based fundamental ruler at the nanoscale. It relies on the efficient energy-transfer mechanism between single quantum emitters and low-doped graphene monolayers.
Cataliotti, F. S.   +10 more
core   +2 more sources

Patterning the Void: Combining L‐Systems with Archimedean Tessellations as a Perspective for Tissue Engineering Scaffolds

open access: yesAdvanced Functional Materials, EarlyView.
This study introduces a novel multi‐scale scaffold design using L‐fractals arranged in Archimedean tessellations for tissue regeneration. Despite similar porosity, tiles display vastly different tensile responses (1–100 MPa) and deformation modes. In vitro experiments with hMSCs show geometry‐dependent growth and activity. Over 55 000 tile combinations
Maria Kalogeropoulou   +4 more
wiley   +1 more source

Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics

open access: yesnpj Imaging
Single-molecule localization microscopy has revealed cellular architectures and molecular dynamics beyond the diffraction limit of light. However, imaging thick samples presents challenges from increased fluorescence background. Light sheet illumination,
Siyang Cheng   +4 more
doaj   +1 more source

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. [PDF]

open access: yes, 2017
Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are
Adli, Mazhar   +9 more
core   +1 more source

Transparent Inorganic–Organic Bilayer Neural Electrode Array and Integration to Miniscope System for In Vivo Calcium Imaging and Electrophysiology

open access: yesAdvanced Functional Materials, EarlyView.
This study presents the BioCLEAR system, a highly transparent and conductive neural electrode array composed of silver nanowires (AgNWs) and doped PEDOT:PSS, enabling neural recordings with minimal optical artifacts. When integrated with a GRIN lens, this cost‐effective neural implant allows simultaneous electrophysiological recording and GCaMP6‐based ...
Dongjun Han   +17 more
wiley   +1 more source

Toward high-content/high-throughput imaging and analysis of embryonic morphogenesis [PDF]

open access: yes, 2011
In vivo study of embryonic morphogenesis tremendously benefits from recent advances in live microscopy and computational analyses. Quantitative and automated investigation of morphogenetic processes opens the field to high-content and high-throughput ...
Supatto, Willy, Truong, Thai V.
core   +4 more sources

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