Results 31 to 40 of about 218,193 (270)
Light-sheets and smart microscopy, an exciting future is dawning
Communications Biology, 2023 Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
doaj +1 more source
Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes. [PDF]
, 2016 The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D 'object plane'.
Bakowski, Malina A +6 more
core +1 more source
Motionless volumetric structured light sheet microscopy. [PDF]
Biomed Opt Express, 2023 To meet the increasing need for low-cost, compact imaging technology with cellular resolution, we have developed a microLED-based structured light sheet microscope for three-dimensional ex vivo and in vivo imaging of biological tissue in multiple modalities.
Peterson T +5 more
europepmc +3 more sources
Deep and fast live imaging with two-photon scanned light-sheet microscopy [PDF]
, 2011 We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large ...
A Edelstein +30 more
core +3 more sources
Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light,
openaire +2 more sources
Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +1 more source
Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy [PDF]
, 2017 Light sheet fluorescence microscopy enables high-resolution imaging of thick biological samples. By restricting the fluorescence excitation to a single plane, rapid wide-field image acquisition is possible with minimal sample exposure.
Corral, Angélica +4 more
core +3 more sources
The light-sheet microscopy revolution
Journal of Optics, 2018 This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging.
J M Girkin, M T Carvalho
openaire +3 more sources
Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]
PLoS Computational Biology, 2018 Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert +4 more
doaj +1 more source
Journal of Integrative Agriculture, 2017 Cell wall composition in monocotyledonous grasses has been identified as a key area of research for developing better feedstocks for forage and biofuel production.
Christopher Brown +2 more
doaj +1 more source