Results 51 to 60 of about 218,193 (270)
Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
doaj +1 more source
Methods and Protocols, 2023 Whole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing.
Youngjae Ryu +5 more
doaj +1 more source
Bessel beam illumination reduces random and systematic errors in quantitative functional studies using light-sheet microscopy [PDF]
, 2018 Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a choice method to observe brain function with high frame rates at cellular resolution.
Alterini, Tommaso +8 more
core +15 more sources
Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser
Methods and Protocols, 2019 Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just ...
Diego Di Battista +4 more
doaj +1 more source
High-speed imaging of amoeboid movements using light-sheet microscopy. [PDF]
PLoS ONE, 2012 Light-sheet microscopy has been developed as a powerful tool for live imaging in biological studies. The efficient illumination of specimens using light-sheet microscopy makes it highly amenable to high-speed imaging. We therefore applied this technology
Daisuke Takao +4 more
doaj +1 more source
Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells [PDF]
, 2013 Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light-sheet microscopy (RLSM), a fluorescence microscopy method allowing ...
Basu, Srinjan +7 more
core +1 more source
Three-photon light-sheet fluorescence microscopy [PDF]
Optics Letters, 2018 We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
openaire +4 more sources
Open-top axially swept light sheet microscopy [PDF]
Biomedical Optics Express, 2020 AbstractOpen-top light sheet microscopy (OT-LSM) is a specialized microscopy for the high throughput cellular imaging of large tissues including optically cleared tissues by having all the optical setup below the sample stage. Current OT-LSM systems had relatively low axial resolutions by using weakly focused light sheets to cover the imaging field of ...
Bumju Kim +7 more
openaire +3 more sources
Axial plane optical microscopy. [PDF]
, 2014 We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning.
Kim, Jeongmin +6 more
core +2 more sources
MEMS enabled miniaturized light-sheet microscopy with all optical control
Scientific Reports, 2021 We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses.
Spyridon Bakas +3 more
doaj +1 more source