Results 211 to 220 of about 73,867 (250)

A deep learning pipeline for three-dimensional brain-wide mapping of local neuronal ensembles in teravoxel light-sheet microscopy. [PDF]

Nat Methods
Attarpour A, Osmann J, Rinaldi A, Qi T, Lal N, Patel S, Rozak M, Yu F, Cho N, Squair J, McLaurin J, Raffiee M, Deisseroth K, Courtine G, Ye L, Stefanovic B, Goubran M.   +16 more
europepmc   +1 more source

Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging. [PDF]

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Zhu E, Li YR, Margolis S, Wang J, Wang K, Zhang Y, Wang S, Park J, Zheng C, Yang L, Chu A, Zhang Y, Gao L, Hsiai TK.   +13 more
europepmc   +1 more source

Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy. [PDF]

Nat Commun
Dibaji H, Kazemi Nasaban Shotorban A, Grattan RM, Lucero S, Schodt DJ, Lidke KA, Petruccelli J, Lidke DS, Liu S, Chakraborty T.   +9 more
europepmc   +1 more source

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Light sheet fluorescence microscopy

Nature Reviews Methods Primers, 2021
Friedrich Preusser, Stephan Preibisch, Reto Fiolka   +2 more
exaly   +2 more sources

Light sheet microscopy

2014
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample ...
Weber, M., Mickoleit, M., Huisken, J.
openaire   +3 more sources

Quantifiable Intravital Light Sheet Microscopy

2022
Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting
Holly C, Gibbs, Sreeja, Sarasamma, Oscar R, Benavides, David G, Green, Nathan A, Hart, Alvin T, Yeh, Kristen C, Maitland, Arne C, Lekven   +7 more
openaire   +2 more sources

An eye on light-sheet microscopy

2016
This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for in vivo imaging but can as well be used for in toto ...
D. Kromm, T. Thumberger, J. Wittbrodt
openaire   +2 more sources

Single-objective light sheet microscopy

Nature Methods, 2021
Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.
openaire   +2 more sources

Fast Fluorescence Microscopy with Light Sheets

The Biological Bulletin, 2016
In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
openaire   +3 more sources

A Practical Guide to Light Sheet Microscopy

2016
Light sheet fluorescence microscopy is an efficient method for imaging large volumes of biological tissue, including brains of larval zebrafish, at high spatial and fairly high temporal resolution with minimal phototoxicity.Here, we provide a practical guide for those who intend to build a light sheet microscope for fluorescence imaging in live larval ...
Davis V, Bennett, Misha B, Ahrens
openaire   +2 more sources