Results 41 to 50 of about 251,658 (355)
Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance
Scientific Reports, 2016 Increasing numbers of materials have been extensively used as platforms for enzyme immobilization to improve catalytic performance. However, activity of the most of the enzymes was declined after immobilization.
J. Cui +4 more
semanticscholar +1 more source
The potential roles of transacylation in intracellular lipolysis and related QSSA approximations [PDF]
arXiv, 2022 Fatty acids (FAs) are crucial energy metabolites, signalling molecules, and membrane building blocks for a wide range of organisms. Adipose triglyceride lipase (ATGL) is the first and presumingly most crucial regulator of FA release from triacylglycerols (TGs) stored within cytosolic lipid droplets.
arxiv
Acid digestion and symbiont: Proton sharing at the origin of mitochondriogenesis?
BioEssays, Volume 45, Issue 1, January 2023., 2023 ADMit hypothesis for the origin of eukaryotes. A bacterial symbiont became associated with a protophagocytic host that captured prey with membrane processes. The symbiont cooperated to kill and digest the prey with reactive oxygen species and the acidification of the microenvironment.
Mario Mencía
wiley +1 more source
Hayati Journal of BiosciencesBacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in ...
Esti Puspitasari +5 more
doaj +1 more source
A Computational Methodology to Screen Activities of Enzyme Variants [PDF]
, 2012 We present a fast computational method to efficiently screen enzyme activity. In the presented method, the effect of mutations on the barrier height of an enzyme-catalysed reaction can be computed within 24 hours on roughly 10 processors. The methodology is based on the PM6 and MOZYME methods as implemented in MOPAC2009, and is tested on the first step
arxiv +1 more source
FEMS Microbiology Reviews, 1994 Many different bacterial species produce lipases which hydrolyze esters of glycerol with preferably long-chain fatty acids. They act at the interface generated by a hydrophobic lipid substrate in a hydrophilic aqueous medium. A characteristic property of lipases is called interfacial activation, meaning a sharp increase in lipase activity observed when
K E, Jaeger +5 more
openaire +2 more sources
Polyphenolic Compounds as Pancreatic Lipase Inhibitors
Planta Medica, 2015 Obesity and its associated diseases such as diabetes mellitus and coronary heart diseases are a major challenge for our society. An important target for the treatment of obesity includes the development of inhibitors of nutrient digestion and absorption.
T. Buchholz, M. Melzig
semanticscholar +1 more source
Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
Biotechnology Reports, 2020 Lipase was immobilized onto bacterial magnetosomes using glutaraldehyde cross-linking and confirmed by Fourier transform infrared spectrometry (FT-IR) and Scanning electron microscopy (SEM).
Jobin John Jacob, K Suthindhiran
doaj
In silico screening of 393 mutants facilitates enzyme engineering of amidase activity in CalB [PDF]
, 2012 Our previously presented method for high throughput computational screening of mutant activity (Hediger et al., arXiv:1203.2950) is benchmarked against experimentally measured amidase activity for 22 mutants of Candida antarctica lipase B (CalB). Using an appropriate cutoff criterion for the computed barriers, the qualitative activity of 15 out of 22 ...
arxiv +1 more source
Toward Design Principles for Biomolecular Condensates for Metabolic Pathways
Advanced Biology, EarlyView.Biomolecular condensates are membrane‐less compartments found through‐out nature which can serve as reaction crucibles for biochemical processes. This review explores the design strategies underlying how condensates can be used in biotechnology to enhance multistep enzyme cascades including enhancement by mass action and substrate channeling, and ...
Alain A.M. André +3 more
wiley +1 more source