Results 71 to 80 of about 17,356 (288)

Cellular palmitoylation and trafficking of lipidated peptides

Journal of Lipid Research, 2007
Many important signaling proteins require the posttranslational addition of fatty acid chains for their proper subcellular localization and function.
Jeremiah M. Draper, Zuping Xia, Charles D. Smith   +2 more
doaj   +1 more source

The Carboxyl-Terminal Amino Acids Render Pro-Human LC3B Migration Similar to Lipidated LC3B in SDS-PAGE [PDF]

, 2013
LC3 is widely used marker for macroautophagy assays. After translation pro-LC3 is processed by Atg4 to expose C-terminal glycine residue for downstream conjugation reactions to accomplish the conversion of LC3-I to LC3-II. SDS-PAGE based Western blot (Wb)
Wang, W, Billiar, Timothy R., Chen, Z, Gao Wentao, Wang, Wei, Timothy R Billiar, Billiar, TR, Billiar Timothy R., Gao, Wentao, Zhixia Chen, Wentao Gao, Wang Wei, Stang Michael T., Chen, Zhixia, Michael T Stang, Timothy R. Billiar, Gasset, Maria, Michael T. Stang, Gao, W, Wei Wang, Stang, MT, Stang, Michael T., Chen Zhixia   +22 more
core   +2 more sources

Image Dataset for Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy

, 2021
Selective autophagy of damaged mitochondria, protein aggregates, and other cargoes is essential for health. Cargo initiates phagophore biogenesis, which entails the conjugation of LC3 to phosphatidylethanolamine.
Chunmei Chang
core   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Crimean-Congo hemorrhagic fever virus replication imposes hyper-lipidation of MAP1LC3 in epithelial cells.

, 2020
International audienceCrimean-Congo hemorrhagic fever virus (CCHFV) is a virus that causes severe liver dysfunctions and hemorrhagic fever, with high mortality rate.
Glaucia Paranhos-Baccalà (3310710), Paranhos-Baccalà, Glaucia, Viret, C, Peyrefitte, Christophe, Rozières, A, Olivier Ferraris (168507), Verlhac, P, Aurore Rozières (116424), Carbonnelle, C, Paranhos-Baccalà, G, Carbonnelle, Caroline, Verlhac, Pauline, Rozières, Aurore, Caroline Carbonnelle (2549053), Hubert P. Endtz (139857), Christophe N. Peyrefitte (8227071), Christophe Viret (8227074), Ferraris, O, Mély, Stéphane, Faure, M, Marie Moroso (4477468), Moroso, M, Endtz, Hubert, Mély, S, Pauline Verlhac (463640), Peyrefitte, Cn, Viret, Christophe, Moroso, Marie, Faure, Mathias, Endtz, Hp, Mathias Faure (193265), Ferraris, Olivier, Stéphane Mély (8227068)   +32 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy

Autophagy Reports
Malaria parasites encounter diverse conditions as they transition between mosquito and mammalian hosts. A characteristic of the sporozoite stage of the parasite is that once it enters the hepatocyte, it changes its morphology and metabolism.
Suryansh Rajput, Satish Mishra
doaj   +1 more source

Protein lipidation meets proteomics.

, 2008
International audienceProtein lipidation is a crucial protein modification involving the attachment of hydrophobic carbon skeletons (C:14-C:60) of various lipid classes--fatty acids, sterols, glycero-, phospho- and glycolipids.
Meinnel, Thierry, Giglione, Carmela
core