Results 21 to 30 of about 333,263 (243)
Visualizing liquid-liquid phase separation and protein aggregates [PDF]
Communications ChemistryLiquid-liquid phase separation (LLPS) facilitates functionally-relevant proteins to gather in high concentrations at specific locations. Several interacting proteins encapsulated in the membraneless droplets tend to form abnormal protein aggregates which
Jing Xue +5 more
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Liquid-liquid phase separation driven by charge heterogeneity
Communications PhysicsDespite the intrinsic charge heterogeneity of proteins plays a crucial role in the liquid-liquid phase separation (LLPS) of a broad variety of protein systems, our understanding of the effects of their electrostatic anisotropy is still in its early ...
Daniele Notarmuzi, Emanuela Bianchi
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Artificial Metalloenzymes in Artificial Sanctuaries Through Liquid–Liquid Phase Separation [PDF]
Bio-ProtocolArtificial metalloenzymes (ArMs) hold great promise for expanding the toolbox of non-natural transformations usable in living systems, such as cells, plants, and animals.
Kaixin Wang +4 more
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Liquid–Liquid Phase Separation in Crowded Environments [PDF]
International Journal of Molecular Sciences, 2020 Biomolecular condensates play a key role in organizing cellular fluids such as the cytoplasm and nucleoplasm. Most of these non-membranous organelles show liquid-like properties both in cells and when studied in vitro through liquid–liquid phase separation (LLPS) of purified proteins.
Alain A. M. André, Evan Spruijt
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Liquid–liquid phase separation in autophagy
Journal of Cell Biology, 2020 Liquid–liquid phase separation (LLPS) compartmentalizes and concentrates biomacromolecules into distinct condensates. Liquid-like condensates can transition into gel and solid states, which are essential for fulfilling their different functions. LLPS plays important roles in multiple steps of autophagy, mediating the assembly of autophagosome formation
Nobuo N. Noda, Zheng Wang, Hong Zhang
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Liquid–liquid phase separation in artificial cells [PDF]
Interface Focus, 2018 Abstract Liquid–liquid phase separation (LLPS) in biology is a recently appreciated means of intracellular compartmentalization. Because the mechanisms driving phase separations are grounded in physical interactions, they can be recreated within less complex systems consisting of only a few simple components, to serve as artificial ...
Charles D. Crowe, Christine D. Keating
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Kinase regulation by liquid–liquid phase separation
Trends in Cell Biology, 2023 Liquid-liquid phase separation (LLPS) is emerging as a mechanism of spatiotemporal regulation that could answer long-standing questions about how order is achieved in biochemical signaling. In this review we discuss how LLPS orchestrates kinase signaling, either by creating condensate structures that are sensed by kinases or by direct LLPS of kinases ...
Tania P. López-Palacios +1 more
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Microfluidic characterization of macromolecular liquid-liquid phase separation [PDF]
Lab on a Chip, 2020 Abstract Liquid-liquid phase separation plays important roles in the compartmentalization of cells. Developing an understanding of how phase separation is encoded in biomacromolecules requires quantitative mapping of their phase behavior.
Anne Bremer +2 more
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Elastic ripening and inhibition of liquid–liquid phase separation [PDF]
Nature Physics, 2020 Phase separation has recently emerged as an important organizational principle in the dense and heterogeneous environment within living cells. Here, we use a synthetic system to show that compressive stresses in a polymer network suppress phase separation of the solvent that swells it. These stresses create a barrier to droplet nucleation that leads to
Kathryn A. Rosowski +5 more
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Modeling Elastically Mediated Liquid-Liquid Phase Separation [PDF]
Physical Review Letters, 2020 We propose a continuum theory of the liquid-liquid phase separation in an elastic network where phase-separated microscopic droplets rich in one fluid component can form as an interplay of fluids mixing, droplet nucleation, network deformation, thermodynamic fluctuation, \emph{etc}.
Xuefeng Wei +3 more
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