Results 31 to 40 of about 342,327 (294)

Kinase regulation by liquid–liquid phase separation

Trends in Cell Biology, 2023
Liquid-liquid phase separation (LLPS) is emerging as a mechanism of spatiotemporal regulation that could answer long-standing questions about how order is achieved in biochemical signaling. In this review we discuss how LLPS orchestrates kinase signaling, either by creating condensate structures that are sensed by kinases or by direct LLPS of kinases ...
Tania P. López-Palacios, Joshua L. Andersen   +1 more
openaire   +2 more sources

Microfluidic characterization of macromolecular liquid-liquid phase separation [PDF]

Lab on a Chip, 2020
Abstract Liquid-liquid phase separation plays important roles in the compartmentalization of cells. Developing an understanding of how phase separation is encoded in biomacromolecules requires quantitative mapping of their phase behavior.
Anne Bremer, Tanja Mittag, Michael Heymann   +2 more
openaire   +3 more sources

Elastic ripening and inhibition of liquid–liquid phase separation [PDF]

Nature Physics, 2020
Phase separation has recently emerged as an important organizational principle in the dense and heterogeneous environment within living cells. Here, we use a synthetic system to show that compressive stresses in a polymer network suppress phase separation of the solvent that swells it. These stresses create a barrier to droplet nucleation that leads to
Kathryn A. Rosowski, Tianqi Sai, Estefania Vidal-Henriquez, David Zwicker, Robert W. Style, Eric R. Dufresne   +5 more
openaire   +5 more sources

Enhancement of DNA Droplet Formation Ability in Liquid–Liquid Phase Separation by Introduction of Artificial Nucleobase [PDF]

, 2021
Controlling liquid–liquid phase separation (LLPS) of biomolecules is a challenge for understanding its biological phenomena and developing drugs for LLPS associated diseases. We found that substitution of DNA bases with 5-nitroindoles, known as universal
Tomohiro, Kitagawa, Ryu, Tashiro, Yuta, Ito   +2 more
core   +1 more source

Modeling Elastically Mediated Liquid-Liquid Phase Separation [PDF]

Physical Review Letters, 2020
We propose a continuum theory of the liquid-liquid phase separation in an elastic network where phase-separated microscopic droplets rich in one fluid component can form as an interplay of fluids mixing, droplet nucleation, network deformation, thermodynamic fluctuation, \emph{etc}.
Xuefeng Wei, Jiajia Zhou, Yanting Wang, Fanlong Meng   +3 more
openaire   +3 more sources

Temperature-Controlled Microchip Liquid Chromatography System [PDF]

, 2006
High-performance liquid chromatography (HPLC) is one of the most important analytical tools heavily used in the fields of chemistry, biotechnology, pharmaceutics, and the food industry.
Shih, Victor-Chi-Yuan
core   +1 more source

Separation of p-Nitrophenol and o-Nitrophenol with three-liquid-phase extraction system [PDF]

, 2009
Three-liquid-phase systems containing polyethylene glycol with different molecular weight were explored to investigate the partitioning behavior of simulated wastewater-containing isomers of p-Nitrophenol (p-NP) and o-Nitrophenot (o-NP). The distribution
Yu, Pinhua, Hua, Chao, Chang, ZD, Liu, Huizhou, Chang, Zhidong, Cao, Hongbin, Ma, Yinchen, Wang, Senjian   +7 more
core   +1 more source

Comprehensive Regulation of Liquid–Liquid Phase Separation of Polypeptides

Molecules, 2023
The elucidation of the molecular driving forces responsible for Liquid–liquid Phase Separation (LLPS) of proteins and nucleic acids within living cells is crucial for understanding its biological functions and its role in related diseases. In the present
Yanwei Wang, Dongxin Xiang, Siyuan Chen, Guangcan Yang   +3 more
doaj   +1 more source

Liquid-Liquid Phase Separation for Analyte Enrichment in Coacervates for Ultra-Sensitive Biosensing [PDF]

, 2023
Efficient isolation and concentration of biomolecules are foundational for rapid diagnostics. Traditional methods, often reliant on solid surfaces, necessitate complex and labor-intensive procedures.
Si , Meng, Chaofeng, Cen, Xudong , Ma, Xi , Lu, Wenwen , Chen, Hui , Zhou, Zhou , Liu, Tiantian, Kong, Yinliang , Lin, cheng, qi   +9 more
core   +1 more source

CTP-controlled liquid–liquid phase separation of ParB

Journal of Molecular Biology, 2022
The ParABS system is supposed to be responsible for plasmid partitioning and chromosome segregation in bacteria. ParABS ensures a high degree of fidelity in inheritance by dividing the genetic material equally between daughter cells during cell division.
Babl, L., Giacomelli, G., Ramm, B., Gelmroth, A., Bramkamp, M., Schwille, P.   +5 more
openaire   +4 more sources