Results 41 to 50 of about 6,019,478 (263)

Mitigation of liquid-liquid phase separation of a monoclonal antibody by mutations of negative charges on the Fab surface.

PLoS ONE, 2020
Some monoclonal antibodies undergo liquid-liquid phase separation owing to self-attractive associations involving electrostatic and other soft interactions, thereby rendering monoclonal antibodies unsuitable as therapeutics.
Tatsuji Matsuoka, Ryuki Miyauchi, Nobumi Nagaoka, Jun Hasegawa   +3 more
doaj   +1 more source

Liquid-liquid crystalline phase separation of evolving amyloid fibrils

Physical Review Research, 2023
We study the conditions necessary for the onset of liquid-liquid crystalline phase separation (LLCPS) in a system of dynamically evolving amyloid fibrils whose kinetics is controlled by nucleation, growth and fragmentation.
Paride Azzari, Raffaele Mezzenga
doaj   +1 more source

LEF1 enhances β-catenin transactivation through IDR-dependent liquid–liquid phase separation

Life Science Alliance, 2023
This research elucidates the correlation between liquid–liquid phase separation (LLPS) of LEF1 with β-catenin and their transcriptional activation function, offering a novel therapeutic approach for the treatment of colorectal cancer through LLPS.
Bing Zhao, Zhuoxin Li, Shaoqing Yu, Tingting Li, Wen Wang, Ran Liu, Biyu Zhang, Xiya Fang, Yezhuang Shen, Qiuying Han, Xin Xu, Kai Wang, Weili Gong, Tao Li, Ailing Li, Tao Zhou, Weihua Li, Teng Li   +17 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Liquid-Liquid Phase Separation in an Elastic Network

Physical Review X, 2018
Living and engineered systems rely on the stable coexistence of two interspersed liquid phases. Yet, surface tension drives their complete separation. Here, we show that stable droplets of uniform and tunable size can be produced through arrested phase ...
Robert W. Style, Tianqi Sai, Nicoló Fanelli, Mahdiye Ijavi, Katrina Smith-Mannschott, Qin Xu, Lawrence A. Wilen, Eric R. Dufresne   +7 more
doaj   +1 more source

Spatiotemporal regulation of insulin signaling by liquid–liquid phase separation

Cell Discovery, 2022
Insulin signals through its receptor to recruit insulin receptor substrates (IRS) and phosphatidylinositol 3-kinase (PI3K) to the plasma membrane for production of phosphatidylinositol-3,4,5-trisphosphate (PIP3) from phosphatidylinositol-4,5-bisphosphate
Kun Zhou, Qiaoli Chen, Jiamou Chen, Derong Liang, Weikuan Feng, Minjun Liu, Qi Wang, Ruizhen Wang, Qian Ouyang, Chao Quan, Shuai Chen   +10 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source