Results 61 to 70 of about 79,026 (218)

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

Advanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells

Journal of Extracellular Vesicles, 2020
Mast cells (MCs) are known to participate in a variety of patho-physiological processes depending largely on the intragranular mediators and the production of cytokines and chemokines during degranulation. Recently, extracellular vesicles (EVs) have been
Yuting Liang, Sheng Huang, Longwei Qiao, Xia Peng, Chong Li, Kun Lin, Guogang Xie, Jia Li, Lihui Lin, Yue Yin, Huanjin Liao, Qian Li, Li Li   +12 more
doaj   +1 more source

FTSJ3‐Mediated 2′‐O‐Methylation of HSPA5 mRNA Stabilizes BOP1 via SUMOylation to Drive DRP1‐Dependent Mitochondrial Fission and Fatty Acid Oxidation in Lung Squamous Cell Carcinoma

Advanced Science, EarlyView.
The RNA methyltransferase FTSJ3 drives lung squamous cell carcinoma progression by catalyzing 2′‐O‐methylation of HSPA5 mRNA, which stabilizes BOP1 through SUMOylation and activates DRP1/SREBP1‐mediated mitochondrial fission and fatty acid oxidation. Targeting this axis with liposomal siRNA potently suppresses tumor growth and metastasis ABSTRACT Lung ...
Qin Hu, Jiaheng Lin, Yifei Zhu, Ying Liu, Guoren Zhou   +4 more
wiley   +1 more source

SAT1‐Induced Spermidine Depletion Potentiates Food Allergy

Advanced Science, EarlyView.
Spermidine depletion, driven by upregulated spermidine/spermine N1‐acetyltransferase (SAT1), potentiates food allergy by promoting mast cell degranulation through autophagy suppression. SAT1 physically interacts with the long noncoding RNA Cdk19os to inhibit Atg5 and Atg7, disrupting autophagic flux.
Manman Liu, Beiyu Zhang, Junjuan Wang, Yue Jiang, Shiwen Han, Qianwei Wang, Lingyu Zeng, Jinfeng Wang, Huilian Che   +8 more
wiley   +1 more source

GSLR Drives Lung Adenocarcinoma as a Critical Regulator of Energy Homeostasis Through Creatine Kinase B Activation

Advanced Science, EarlyView.
This study identifies the glucose/glutamine sensitive lncRNA GSLR as a driver of lung adenocarcinoma progression. Nutrient restriction reduces chromatin accessibility at the GSLR locus and blocks CTCF‐mediated transcriptional regulation. GSLR directly interacts with DHX9 and recruits it to the CKB promoter, arresting R‐loop accumulation and promoting ...
Xinyi Qian, Juze Yang, Jiayi Ren, Xiaowei Mao, Jia Li, Tengfei Sun, Liangliang Dong, Enguo Chen, Yan Lu, Pengyuan Liu   +9 more
wiley   +1 more source

Decoding Biomolecular Phase Separation: From Physiological Roles to Pathological Mechanisms and Therapeutic Biomaterials Design

Advanced Science, EarlyView.
This review systematically elaborates on the mechanisms underlying physiological regulation and pathological dysregulation of biomolecular phase separation. It also summarizes advances in phase‑separation‑targeted therapeutic strategies and the design of functional biomaterials inspired by this process.
Jiawei Zhu, Jingbo Dong, Qiufang Gong, Xuejiao Song, Zhifa Shen, Chao Liang   +5 more
wiley   +1 more source

Bioinformatics-Based Screening of Key LncRNAs for Modulating the Transcriptome Associated with Glaucoma in Human Trabecular Meshwork Cells

Frontiers in Bioscience-Landmark
Objective: The morphology and functions of the human trabecular meshwork (HTM) are dysregulated in glaucoma, and the molecular mechanisms of this dysregulation remain unknown.
Junhong Guo, Yunfei Wu, Yue Sun, Dong Chen, Yijia Huang, Xiaoli Shen, Zhichao Yan, Jiantao Wang   +7 more
doaj   +1 more source

Transcriptomic Analysis of Trigeminal Ganglion and Spinal Trigeminal Nucleus Caudalis in Mice with Inflammatory Temporomandibular Joint Pain

Journal of Pain Research, 2022
Tieli Dong,1,* Haichao Si,1,2,* Zhisong Li,1 Qian Bai,1 Feng Tao3 1Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China; 2Department of Anesthesiology, Nanyang Central ...
Dong T, Si H, Li Z, Bai Q, Tao F
doaj  

Identification and Functional Characterization of PI3K/Akt/mTOR Pathway-Related lncRNAs in Lung Adenocarcinoma: A Retrospective Study [PDF]

Cell Journal
Objective: This paper aimed to investigate the PI3K/Akt/mTOR signal-pathway regulator factor-related lncRNAsignatures (PAM-SRFLncSigs), associated with regulators of the indicated signaling pathway in patients with lungadenocarcinoma (LUAD) undergoing ...
Jiaqi Zhong, Ying Kong, Ruming Li, Minghan Feng, Liming Li, Xiao Zhu, Lianzhou Chen   +6 more
doaj   +1 more source

SEC Mediates m6A Deposition and Transcription Pause Release to Drive Cell Identity Transition

Advanced Science, EarlyView.
The SEC promotes cell identity transitions by facilitating transcription pause release at key identity‐associated genes, including Klf4 and Myc during iPSC reprogramming and Nes and Tubb3 during ESC‐to‐neuroectodermal differentiation. During early iPSC reprogramming, this function additionally involves cooperation with METTL3‐associated m6A regulation.
Zhijing Zhang, Jingyi Xu, Yonghong Wu, Yitong Qu, Xinglin Hu, Yuchen Sun, Yanshuang Wu, Zhonghua Liu, Jiaqiang Wang, Lei Lei   +9 more
wiley   +1 more source