Results 11 to 20 of about 672,835 (295)
Long-Read Sequencing Emerging in Medical Genetics [PDF]
Frontiers in Genetics, 2019 The wide implementation of next-generation sequencing (NGS) technologies has revolutionized the field of medical genetics. However, the short read lengths of currently used sequencing approaches pose a limitation for the identification of structural ...
Tuomo Mantere +7 more
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Long-read sequencing for brain tumors [PDF]
Frontiers in OncologyBrain tumors and genomics have a long-standing history given that glioblastoma was the first cancer studied by the cancer genome atlas. The numerous and continuous advances through the decades in sequencing technologies have aided in the advanced ...
William J. Shelton +8 more
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Long-Read Nanopore-Based Sequencing of Anelloviruses
VirusesRoutinely used metagenomic next-generation sequencing (mNGS) techniques often fail to detect low-level viremia (
Raghavendran Anantharam +6 more
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Genome sequencing using long-read sequencing
Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 2023 Juan Guillermo McEwen +1 more
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Improving Bacterial Metagenomic Research through Long-Read Sequencing
Microorganisms, 2023 Metagenomic sequencing analysis is central to investigating microbial communities in clinical and environmental studies. Short-read sequencing remains the primary approach for metagenomic research; however, long-read sequencing may offer advantages of ...
Noah Greenman +4 more
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Engineering psychrophilic polymerase for nanopore long-read sequencing
Frontiers in Bioengineering and BiotechnologyUnveiling the potential application of psychrophilic polymerases as candidates for polymerase-nanopore long-read sequencing presents a departure from conventional choices such as thermophilic Bacillus stearothermophilus (Bst) renowned for its limitation ...
Yaping Sun +6 more
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Nanopore long-read sequencing of circRNAs [PDF]
Methods, 2021 Circular RNA (circRNA) is a group of highly stable RNA molecules with suggested roles in development and disease. They derive from linear pre-mRNAs when a 5'-splice site splices back to an upstream 3'-splice site in a process termed back-splicing. Most circRNAs are multi-exonic and may contain several thousand nucleotides.
Rahimi, Karim +3 more
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Towards population-scale long-read sequencing [PDF]
Nature Reviews Genetics, 2021 Long-read sequencing technologies have now reached a level of accuracy and yield that allows their application to variant detection at a scale of tens to thousands of samples. Concomitant with the development of new computational tools, the first population-scale studies involving long-read sequencing have emerged over the past 2 years and, given the ...
Wouter De Coster +2 more
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Deciphering Neurodegenerative Diseases Using Long-Read Sequencing [PDF]
Neurology, 2021 Neurodegenerative diseases exhibit chronic progressive lesions in the central and peripheral nervous systems with unclear causes. The search for pathogenic mutations in human neurodegenerative diseases has benefited from massively parallel short-read sequencers.
Su, Yun +12 more
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Long-read sequencing in human genetics [PDF]
Medizinische Genetik, 2019 Abstract Sanger sequencing revolutionized molecular genetics 40 years ago. However, next-generation sequencing technologies became further game changers and shaped our current view on genome structure and function in health and disease.
Kraft, Florian, Kurth, Ingo
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