Results 111 to 120 of about 9,152,726 (291)

Genomic surveillance of multidrug-resistant organisms based on long-read sequencing

Genome Medicine
Background Multidrug-resistant organisms (MDRO) pose a significant threat to public health worldwide. The ability to identify antimicrobial resistance determinants, to assess changes in molecular types, and to detect transmission are essential for ...
Fabian Landman, Casper Jamin, Angela de Haan, Sandra Witteveen, Jeroen Bos, Han G. J. van der Heide, Leo M. Schouls, Antoni P. A. Hendrickx, Dutch CPE/MRSA surveillance study group   +8 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Leveraging the power of long reads for targeted sequencing

Genome Research
Long-read sequencing technologies have improved the contiguity and, as a result, the quality of genome assemblies by generating reads long enough to span and resolve complex or repetitive regions of the genome. Several groups have shown the power of long reads in detecting thousands of genomic and epigenomic features that were previously missed by ...
Shruti V. Iyer, Sara Goodwin, William Richard McCombie   +2 more
openaire   +3 more sources

Improving Bacterial Metagenomic Research through Long-Read Sequencing

Microorganisms
Metagenomic sequencing analysis is central to investigating microbial communities in clinical and environmental studies. Short-read sequencing remains the primary approach for metagenomic research; however, long-read sequencing may offer advantages of ...
Noah Greenman, Sayf Al-Deen Hassouneh, Latifa S. Abdelli, Catherine Johnston, Taj Azarian   +4 more
doaj   +1 more source

Performance of neural network basecalling tools for Oxford Nanopore sequencing

Genome Biology, 2019
Background Basecalling, the computational process of translating raw electrical signal to nucleotide sequence, is of critical importance to the sequencing platforms produced by Oxford Nanopore Technologies (ONT).
Ryan R. Wick, Louise M. Judd, Kathryn E. Holt   +2 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Dynamic Transcriptome Sequencing of Bovine Alphaherpesvirus Type 1 and Host Cells Carried Out by a Multi-Technique Approach

Frontiers in Genetics, 2021
Dóra Tombácz, Norbert Moldován, Gábor Torma, Tibor Nagy, Ákos Hornyák, Zsolt Csabai, Gábor Gulyás, Miklós Boldogkői, Victoria A. Jefferson, Zoltán Zádori, Florencia Meyer, Zsolt Boldogkői   +11 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Analýza nástrojů a pipeline pro zpracování long-read sequencing dat

, 2023
Long-read sekvenování je technologie, která způsobila revoluci v oblasti genomiky. Na rozdíl od dříve používaných sekvenačních technik, které dokážou přečíst pouze krátké úseky DNA, long-read sekvenování dokáže číst až desítky tisíc bází najednou, což ...
Soukupová, Barbora
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