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Transcript Identification Through Long-Read Sequencing
2021RNA-seq using long-read sequencing, such as nanopore and SMRT (Single Molecule, Real-Time) sequencing, enabled the identification of the full-length structure of RNA molecules. Several tools for long-read RNA-seq were developed recently. In this section, we introduce an analytical pipeline of long-read RNA-seq for isoform identification and the ...
Masahide, Seki +4 more
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Long-read sequencing of new Drosophila genomes
Nature Reviews Genetics, 2021Kim et al. present a drosophilid genome resource comprising 101 de novo genome assemblies from 93 drosophilid species obtained by nanopore sequencing.
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Assembling Plant Genomes with Long-Read Sequencing
2022Continuous improvements in long-read sequencing allow us to tackle increasingly big and complex genomes. Here we present the principles of long-read genome assembly, taking Solanum pennellii nanopore sequencing as an example.
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Profiling the epigenome using long-read sequencing
Nature GeneticsThe advent of single-molecule, long-read sequencing (LRS) technologies by Oxford Nanopore Technologies and Pacific Biosciences has revolutionized genomics, transcriptomics and, more recently, epigenomics research. These technologies offer distinct advantages, including the direct detection of methylated DNA and simultaneous assessment of DNA sequences ...
Tianyuan Liu, Ana Conesa
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Long-read Sequencing Technology
This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing.openaire +1 more source
Genome sequencing: Long reads for a short plant
Nature Plants, 2015The genome of a tiny resurrection plant has been sequenced using PacBio's long-read single-molecule real-time sequencing technology, aiding the understanding of extreme desiccation tolerance. The genome contiguity is comparable to that of genomes sequenced using far more laborious approaches.
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Long-read sequencing in the era of epigenomics and epitranscriptomics
Nature Methods, 2023Morghan C. Lucas, Eva Maria Novoa
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