Results 261 to 270 of about 9,152,726 (291)
Some of the next articles are maybe not open access.
Iso-Seq Long Read Transcriptome Sequencing
2021Isoform sequencing (Iso-Seq), a long-read transcriptome sequencing method developed by Pacific Biosciences, has the capacity to generate full-length transcripts without the need for assembly. The method facilitates better genome annotation, identification of alternative splicing events, novel transcript isoforms and epi-transcriptome modification which
Hoang, Nam V., Henry, Robert J.
openaire +2 more sources
Transcript Identification Through Long-Read Sequencing
2021RNA-seq using long-read sequencing, such as nanopore and SMRT (Single Molecule, Real-Time) sequencing, enabled the identification of the full-length structure of RNA molecules. Several tools for long-read RNA-seq were developed recently. In this section, we introduce an analytical pipeline of long-read RNA-seq for isoform identification and the ...
Masahide, Seki +4 more
openaire +2 more sources
Amplicon Sequencing using the Long-Read Sequencing Technologies
Journal of Visualized ExperimentsThe World Health Organization (WHO) continues to emphasize the urgent need for a rapid, cost-effective, and user-friendly diagnostic method for tuberculosis (TB) and drug-resistant TB (DR-TB). Next-generation sequencing (NGS) technologies, endorsed by the WHO, have significantly improved the detection of DR-TB.
Morwasehla, Modjadji +7 more
openaire +2 more sources
Long-read sequencing of new Drosophila genomes
Nature Reviews Genetics, 2021Kim et al. present a drosophilid genome resource comprising 101 de novo genome assemblies from 93 drosophilid species obtained by nanopore sequencing.
openaire +2 more sources
Assembling Plant Genomes with Long-Read Sequencing
2022Continuous improvements in long-read sequencing allow us to tackle increasingly big and complex genomes. Here we present the principles of long-read genome assembly, taking Solanum pennellii nanopore sequencing as an example.
openaire +2 more sources
Long-read Sequencing Technology
This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing.openaire +1 more source
Genome sequencing: Long reads for a short plant
Nature Plants, 2015The genome of a tiny resurrection plant has been sequenced using PacBio's long-read single-molecule real-time sequencing technology, aiding the understanding of extreme desiccation tolerance. The genome contiguity is comparable to that of genomes sequenced using far more laborious approaches.
openaire +2 more sources
Long-read sequencing in the era of epigenomics and epitranscriptomics
Nature Methods, 2023Morghan C. Lucas, Eva Maria Novoa
openaire +2 more sources