Results 111 to 120 of about 35,344 (268)

Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR

Practical Laboratory Medicine
Isothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee, Tesfaye Sisay Tessema   +1 more
doaj   +1 more source

Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea [PDF]

A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at ...
Behzadbehbahani, Chaudhry, Chernesky, Francois, G. Hobbs, Geraats-Peters, H. B. Smalley, Hedman, Kaneko, Khan, L. Gillies, Luijt, Nakao, Newman, Njiru, Notomi, P. A. Burke, T. Edwards, Tomita, World Health Organization   +19 more
core   +1 more source

Polyethylene Glycol Accelerates Loop-mediated Isothermal Amplification (LAMP) Reaction

YAKUGAKU ZASSHI, 2013
Loop-mediated isothermal amplification (LAMP) has several advantages: this technique involves gene amplification under isothermal conditions using only one high-specificity enzyme; the amplification efficiency is so high that large quantities of pyrophosphoric acid are formed as a by-product of DNA synthesis; furthermore, the results can be judged ...
Kenta, Nose, Kentaro, Nagamine, Junichi, Tokuda, Junichi, Takino, Takamitsu, Hori   +4 more
openaire   +3 more sources

Machine Learning‐Augmented Loop‐Mediated Isothermal Amplification‐Enabled Point‐of‐Care for Mpox‐Specific Detection

Advanced Intelligent Systems, EarlyView.
A low‐cost, portable point‐of‐care platform for rapid Mpox detection using loop‐mediated isothermal amplification is reported. The device integrates fluorescence readout and mobile monitoring. A machine‐learning model analyzes temperature data and correlates thermal changes with DNA concentration, enabling sensitive and reliable molecular diagnosis in ...
Nazente Atceken, Ahmed Choukri Abdullah, Olgac Ozarslan, Ziya Yıldız, Savas Tasoglu   +4 more
wiley   +1 more source

Detection of Helicobacter pylori in Drinking Water by Loop-Mediated Isothermal Amplification [PDF]

, 2017
Background: There should be a public environmental reservoir for Helicobacter pylori in the developing countries, such as Iran, due to their high infection rate of over 70%.
Abiri, Ramin., Alvandi, Amirhooshang, Azizi, Mohsen., Bagherabadi, Shahram., Gholipour, Abolfazl., Hasanvand, Banafsheh., Kashef, Mahsa., Pajavand, Hamid.   +7 more
core   +1 more source

DNA Flap‐Mediated Control of Transcription for Programmable RNA Synthesis

Angewandte Chemie International Edition, EarlyView.
Pyrimidine‐rich ssDNA flaps on the T7 promoter inhibit T7 RNA polymerase (T7RP) transcription. Leveraging this pyrimidine bias, we developed DNAzyme‐ and MNAzyme‐mediated flap promoter induced transcription control (D‐FIT and M‐FIT) systems. These platforms enable regulated transcription and sequence‐specific detection, highlighting the programmable ...
Eun Sung Lee, Jisu Woo, Seokjoon Kim, Seok Hyeon Kim, Gun Haeng Lee, Ki Soo Park   +5 more
wiley   +1 more source

Monitoring genetic population biomarkers for wastewater-based epidemiology [PDF]

, 2017
We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic
Cooper, Jonathan M., Kasprzyk-Hordern, Barbara, Reboud, Julien, Xu, Gaolian, Yang, Zhugen   +4 more
core   +3 more sources

Allele-Specific Isothermal Amplification Method Using Unmodified Self-Stabilizing Competitive Primers. [PDF]

, 2018
Rapid and specific detection of single nucleotide polymorphisms (SNPs) related to drug resistance in infectious diseases is crucial for accurate prognostics, therapeutics and disease management at point-of-care.
Baum, Jake, Chotivanich, Kesinee, Delves, Michael J, Georgiou, Pantelis, Malpartida-Cardenas, Kenny, Nguon, Chea, Rodriguez-Manzano, Jesus, Yu, Ling-Shan   +7 more
core   +2 more sources

Click Capture SELEX for the Identification of Click‐Modified Aptamers Targeting Small Molecules in Solution

Chemistry – A European Journal, EarlyView.
Using kanamycin A as a model target, we developed click‐capture SELEX to identify nucleobase‐modified aptamers capable of binding small molecules in solution. This approach enables flexible incorporation of nucleobase modifications via CuAAC, allowing the chemical properties of the aptamer to be tailored to the target molecule.
Philipp Menke, Carmelo Di Primo, Felix Bernhardt, Alea Schröder, Christian Renzl, Günter Mayer   +5 more
wiley   +1 more source

Loop-mediated isothermal amplification for detection of porcine circovirus type 2 [PDF]

, 2011
Background Porcine circovirus type 2 (PCV2) is the primary causative agent of the emerging swine disease known as postweaning multisystemic wasting syndrome (PMWS).
Shun Zhou, Si Han, Jianli Shi, Jiaqiang Wu, Xiaoyuan Yuan, Xiaoyan Cong, Shaojian Xu, Xiaoyan Wu, Jun Li, Jinbao Wang   +9 more
core   +2 more sources