Results 31 to 40 of about 35,344 (268)

Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP [PDF]

, 2015
In this paper, we asked if it is possible to identify the best primers and reaction conditions based on improvements in reaction speed when optimizing isothermal reactions.
Ismagilov, Rustem F., Karymov, Mikhail A., Khorosheva, Eugenia M., Selck, David A.   +3 more
core   +2 more sources

Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]

, 2017
Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K., Bosompem, Kwabena M., Lodh, Nilanjan, Mikita, Kei, Otchere, Joseph, Quartey, Joseph K., Shiff, Clive J.   +6 more
core   +2 more sources

Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting

Malaria Journal, 2023
Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance ...
Anna-Clara Ivarsson, Elin Fransén, Ioanna Broumou, Anna Färnert, Kristina E. M. Persson, Sara Karlsson Söbirk   +5 more
doaj   +1 more source

DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

Methods and Protocols, 2018
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease.
Álvaro Rodríguez-García, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Alexei F. Licea-Navarro, Marco A. Ramos   +5 more
doaj   +1 more source

Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples [PDF]

, 2015
Background: Leishmania infantum infections in dogs play a crucial role in the transmission of pathogens causing visceral leishmaniasis to humans in the Gansu province, northwest China. To be able to control zoonotic transmission of the parasite to humans,
A Moshfe, A Nasereddin, AF Koutinas, CH Gao, Chun-hua Gao, D Otranto, D Strauss-Ayali, Dan Ding, Dietmar Steverding, ER Adams, F Dantas-Torres, Feng Shi, G Schönian, H Kaneko, H Takagi, J Alvar, J Dereure, JM Lawrie, Jun-yun Wang, JY Wang, JY Wang, JY Wang, K Stuart, L Chamaillé, L He, L Solano-Gallego, LL Poon, LS Leontides, M Chaouch, M Gramiccia, M Paiva Cavalcanti de, MG Khan, N Chargui, N Kuboki, N Singh, P Ciaramella, RS Leite, S Reale, SA Ferreira, T Muccio Di, T Notomi, UG Lopes, WP Kennedy, X Ni, Xia Wang, Y Fichoux le, Y Mori, YM Gomes, Yue-tao Yang   +48 more
core   +1 more source

Nucleic acid extraction-free isothermal amplification based on magnetic beads capture and ligation [PDF]

Jichu yixue yu linchuang, 2021
Objective Capture and ligation probe-loop-mediated isothermal amplification CLIP-LAMP method with-out nucleic acid extraction based on magnetic beads was established to provide a new technique for the detection of human immunodeficiency virus (HIV) in
QIU Li-zhen, YANG Ming-zhu, PIAN Hong-ru, ZHENG Zhi
doaj  

Diagnostic accuracy of TB-LAMP for pulmonary tuberculosis: a systematic review and meta-analysis. [PDF]

, 2019
BACKGROUND:The need for a rapid, molecular test to diagnose tuberculosis (TB) has prompted exploration of TB-LAMP (Eiken; Tokyo, Japan) for use in resource-limited settings.
Cattamanchi, Adithya, Farr, Katherine, Gray, Christen M, Shete, Priya B, Strnad, Luke   +4 more
core   +2 more sources

Capture and ligation-based loop-mediated isothermal amplification technology [PDF]

Jichu yixue yu linchuang, 2020
Objective To establish a capture and ligation-based loop-mediated isothermal amplification technology (CLIP-LAMP) based on probe capture and ligation reaction to provide a new method for laboratory diagnosis and pathogen detection.
PIAN Hong-ru, YANG Ming-zhu, ZHENG Zhi
doaj  

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

Viruses, 2022
A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections.
Chao Yu, Lulu Zuo, Jing Miao, Lingjing Mao, Benjamin Selekon, Ella Gonofio, Emmanuel Nakoune, Nicolas Berthet, Gary Wong   +8 more
doaj   +1 more source

Asymmetric stem-loop–mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple modification of canonical PCR primers

Frontiers in Bioengineering and Biotechnology, 2022
Nucleic acid–based assays have been adopted as mainstream tools for clinical diagnostics, food safety, and environment monitoring with the merits of accuracy, rapidity, and sensitivity.
Rui Mao, Rui Mao, Xinyao Wu, Xinyao Wu, Qing Miao, Qing Miao, Ting Cai, Ting Cai   +7 more
doaj   +1 more source