PLoS ONE, 2020 Novel Corona virus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or 2019-nCoV), and the subsequent disease caused by the virus (coronavirus disease 2019 or COVID-19), is an emerging global health concern that requires a rapid diagnostic ...
L. Lamb +3 more
semanticscholar +1 more source
Data in Brief, 2018 Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat +5 more
doaj +1 more source
Strategies for the Voltammetric Detection of Loop-Mediated Isothermal Amplification
Micromachines, 2023 Loop-mediated isothermal amplification (LAMP) is rapidly developing into an important tool for the point-of-use detection of pathogens for both clinical and environmental samples, largely due to its sensitivity, rapidity, and adaptability to portable ...
Jesse M. Marangoni +2 more
doaj +1 more source
Capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients [PDF]
, 2016 We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of ...
Cooper, Jonathan M. +3 more
core +1 more source
Loop‐mediated isothermal amplification of single pollen grains [PDF]
Journal of Integrative Plant Biology, 2014 AbstractThe polymerase chain reaction (PCR) has been a reliable and fruitful method for many applications in ecology. Nevertheless, unavoidable technical and instrumental requirements of PCR have limited its widespread application in field situations. The recent development of isothermal DNA amplification methods provides an alternative to PCR, which ...
Ali, Bektaş, Ignacio, Chapela
openaire +2 more sources
Low-Temperature Loop-Mediated Isothermal Amplification Operating at Physiological Temperature
Biosensors, 2023 Loop-mediated isothermal amplification (LAMP) is one of the most widely used isothermal amplification technologies in molecular diagnostics. However, LAMP operates at a high temperature of 65 °C; thus, operating LAMP at a lower temperature is desirable ...
Daehan Nam +9 more
semanticscholar +1 more source
Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula
AMB Express, 2018 Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of ...
Shiqian Fu +6 more
doaj +1 more source
Foods, 2022 The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive
Ye-Ji Moon, So-Young Lee, Se-Wook Oh
doaj +1 more source
Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP [PDF]
, 2015 In this paper, we asked if it is possible to identify the best primers and reaction conditions based on improvements in reaction speed when optimizing isothermal reactions.
Ismagilov, Rustem F. +3 more
core +2 more sources
Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae [PDF]
, 2020 The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly.
Garner, Omai B. +5 more
core +1 more source