A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus [PDF]
The Plant Pathology Journal, 2023 Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, ‘Candidatus Liberibacter asiaticus’ (CLas) is a non-culturable phloem-limited α ...
Tirumalareddy Danda +5 more
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PLoS ONE, 2020 A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou +2 more
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Frontiers in Cellular and Infection Microbiology, 2022 Streptococcus suis serotypes 2 and 14 are the most prevalent zoonotic strains. The establishment of a sensitive and extremely accurate method for point-of-care testing for Streptococcus suis serotype 2 and 14 strains is highly desirable. In this study, a
Jiajia Meng +42 more
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Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification [PDF]
, 2011 A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers
Hao-tai Chen +3 more
core +1 more source
Detecting amplicons of loop‐mediated isothermal amplification [PDF]
Microbiology and Immunology, 2019 AbstractLoop‐mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real‐time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA.
openaire +2 more sources
The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America [PDF]
, 2018 It is estimated that between 8000 and 15 000 Trypanosoma cruzi infected babies are born every year to infected mothers in Chagas disease endemic countries. Currently, poor access to and performance of the current diagnostic algorithm, based on microscopy
Cruz, Israel +7 more
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Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes [PDF]
Journal of Clinical Microbiology, 2003 ABSTRACTWhile PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity,
Noritaka, Kuboki +7 more
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Rapid and sensitive detection of mycobacterium ulcerans by use of a loop-mediated isothermal amplification test [PDF]
, 2012 This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404.
Aiga +24 more
core +1 more source
Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP) [PDF]
Lab on a Chip, 2015 A continuous flow droplet microfluidic device for digital nucleic acid detection using Loop-Mediated Isothermal Amplification (LAMP).
Tushar D, Rane +3 more
openaire +2 more sources
Molecular Methods for Identification and Quantification of Foodborne Pathogens
Molecules, 2022 Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang +7 more
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