Results 41 to 50 of about 52,140 (330)

Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater

Environmental Science and Technology, 2022
Wastewater-based surveillance of the COVID-19 pandemic holds great promise; however, a point-of-use detection method for SARS-CoV-2 in wastewater is lacking.
Haorui Cao, K. Mao, Fang Ran, P. Xu, Yirong Zhao, Xiangyan Zhang, Hourong Zhou, Zhugen Yang, Hua Zhang, Guibin Jiang   +9 more
semanticscholar   +1 more source

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19

Journal of Clinical Microbiology, 2020
The clinical performances of six molecular diagnostic tests and a rapid antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were clinically evaluated for the diagnosis of coronavirus disease 2019 (COVID-19) in self-collected ...
M. Nagura-Ikeda, K. Imai, S. Tabata, K. Miyoshi, Nami Murahara, Tsukasa Mizuno, M. Horiuchi, Kento Kato, Yoshitaka Imoto, Makiko Iwata, S. Mimura, Toshimitsu Ito, Kaku Tamura, Y. Kato   +13 more
semanticscholar   +1 more source

Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples [PDF]

, 2015
Background: Leishmania infantum infections in dogs play a crucial role in the transmission of pathogens causing visceral leishmaniasis to humans in the Gansu province, northwest China. To be able to control zoonotic transmission of the parasite to humans,
A Moshfe, A Nasereddin, AF Koutinas, CH Gao, Chun-hua Gao, D Otranto, D Strauss-Ayali, Dan Ding, Dietmar Steverding, ER Adams, F Dantas-Torres, Feng Shi, G Schönian, H Kaneko, H Takagi, J Alvar, J Dereure, JM Lawrie, Jun-yun Wang, JY Wang, JY Wang, JY Wang, K Stuart, L Chamaillé, L He, L Solano-Gallego, LL Poon, LS Leontides, M Chaouch, M Gramiccia, M Paiva Cavalcanti de, MG Khan, N Chargui, N Kuboki, N Singh, P Ciaramella, RS Leite, S Reale, SA Ferreira, T Muccio Di, T Notomi, UG Lopes, WP Kennedy, X Ni, Xia Wang, Y Fichoux le, Y Mori, YM Gomes, Yue-tao Yang   +48 more
core   +1 more source

Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification

Clinical Chemistry, 2020
Background Rapid, reliable, and widespread testing is required to curtail the ongoing COVID-19 pandemic. Current gold standard nucleic acid tests are hampered by supply shortages in critical reagents including nasal swabs, RNA extraction kits, personal ...
M. Lalli, Joshua S Langmade, Xuhua Chen, C. Fronick, Christopher Sawyer, Lauren C Burcea, Michael N. Wilkinson, R. Fulton, Michael E. Heinz, W. Buchser, R. Head, R. Mitra, J. Milbrandt   +12 more
semanticscholar   +1 more source

Head-to-head comparison of two loop-mediated isothermal amplification (LAMP) kits for diagnosis of malaria in a non-endemic setting

Malaria Journal, 2023
Background Light microscopy and rapid diagnostic tests (RDT) have long been the recommended diagnostic methods for malaria. However, in recent years, loop-mediated isothermal amplification (LAMP) techniques have been shown to offer superior performance ...
Anna-Clara Ivarsson, Elin Fransén, Ioanna Broumou, Anna Färnert, Kristina E. M. Persson, Sara Karlsson Söbirk   +5 more
doaj   +1 more source

Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]

, 2017
Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K., Bosompem, Kwabena M., Lodh, Nilanjan, Mikita, Kei, Otchere, Joseph, Quartey, Joseph K., Shiff, Clive J.   +6 more
core   +2 more sources

Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride.

BioTechniques, 2020
Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment.
Yinhua Zhang, Guoping Ren, J. Buss, A. Barry, Gregory C. Patton, N. Tanner   +5 more
semanticscholar   +1 more source

Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox Virus

Microbiology spectrum, 2022
MPXV outbreaks rapidly grew in the first half of 2022, and this virus has been recognized as an increasing public health threat, particularly in the context of the COVID-19 pandemic.
Junxia Feng, Guan-hua Xue, X. Cui, B. Du, Yanling Feng, J. Cui, Hanqing Zhao, Lin Gan, Zheng Fan, Tongtong Fu, Ziyi Xu, Shuheng Du, Yao Zhou, Rui Zhang, Hanyu Fu, Ziyan Tian, Qun Zhang, Chao Yan, Jing Yuan   +18 more
semanticscholar   +1 more source

DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

Methods and Protocols, 2018
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease.
Álvaro Rodríguez-García, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Alexei F. Licea-Navarro, Marco A. Ramos   +5 more
doaj   +1 more source

Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2

Emerging Microbes and Infections, 2020
The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the role of diagnostics in impediment of further spread and transmission. With the recent emergence of the novel SARS-CoV-2, the availability of rapid, sensitive, and reliable ...
Y. Baek, Jihye Um, K. J. C. Antigua, Ji-Hyun Park, Yeonjae Kim, Sol-La Oh, Young-Il Kim, Won-Suk Choi, Seong-Gyu Kim, J. Jeong, B. Chin, H. D. G. Nicolas, Ji-Young Ahn, K. Shin, Y. Choi, Jun-Sun Park, Min-Suk Song   +16 more
semanticscholar   +1 more source