Results 51 to 60 of about 52,140 (330)

Nucleic acid extraction-free isothermal amplification based on magnetic beads capture and ligation [PDF]

Jichu yixue yu linchuang, 2021
Objective Capture and ligation probe-loop-mediated isothermal amplification CLIP-LAMP method with-out nucleic acid extraction based on magnetic beads was established to provide a new technique for the detection of human immunodeficiency virus (HIV) in
QIU Li-zhen, YANG Ming-zhu, PIAN Hong-ru, ZHENG Zhi
doaj  

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

Viruses, 2022
A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections.
Chao Yu, Lulu Zuo, Jing Miao, Lingjing Mao, Benjamin Selekon, Ella Gonofio, Emmanuel Nakoune, Nicolas Berthet, Gary Wong   +8 more
doaj   +1 more source

Capture and ligation-based loop-mediated isothermal amplification technology [PDF]

Jichu yixue yu linchuang, 2020
Objective To establish a capture and ligation-based loop-mediated isothermal amplification technology (CLIP-LAMP) based on probe capture and ligation reaction to provide a new method for laboratory diagnosis and pathogen detection.
PIAN Hong-ru, YANG Ming-zhu, ZHENG Zhi
doaj  

Diagnostic accuracy of TB-LAMP for pulmonary tuberculosis: a systematic review and meta-analysis. [PDF]

, 2019
BACKGROUND:The need for a rapid, molecular test to diagnose tuberculosis (TB) has prompted exploration of TB-LAMP (Eiken; Tokyo, Japan) for use in resource-limited settings.
Cattamanchi, Adithya, Farr, Katherine, Gray, Christen M, Shete, Priya B, Strnad, Luke   +4 more
core   +2 more sources

Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification

medRxiv, 2020
Novel Corona virus (COVID-19 or 2019-nCoV) is an emerging global health concern that requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is currently the standard for COVID-19 detection; however, Reverse Transcription Loop ...
L. Lamb, S. Bartolone, E. Ward, M. Chancellor   +3 more
semanticscholar   +1 more source

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

Advanced Engineering Materials, 2022
The loop‐mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle.
Nazente Atçeken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, A. Yetisen, S. Tasoglu   +4 more
semanticscholar   +1 more source

Asymmetric stem-loop–mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple modification of canonical PCR primers

Frontiers in Bioengineering and Biotechnology, 2022
Nucleic acid–based assays have been adopted as mainstream tools for clinical diagnostics, food safety, and environment monitoring with the merits of accuracy, rapidity, and sensitivity.
Rui Mao, Rui Mao, Xinyao Wu, Xinyao Wu, Qing Miao, Qing Miao, Ting Cai, Ting Cai   +7 more
doaj   +1 more source

The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America [PDF]

, 2018
It is estimated that between 8000 and 15 000 Trypanosoma cruzi infected babies are born every year to infected mothers in Chagas disease endemic countries. Currently, poor access to and performance of the current diagnostic algorithm, based on microscopy
Cruz, Israel, Katz, Zachary, Ndung'u, Joseph Mathu, Picado, Albert, Redard Jacot, Maël, Schijman, Alejandro Gabriel, Sosa-Estani, Sergio Alejandro, Torrico, Faustino   +7 more
core   +1 more source

Development and Validation of a Rapid, Single-Step Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) System Potentially to Be Used for Reliable and High-Throughput Screening of COVID-19

Frontiers in Cellular and Infection Microbiology, 2020
Objectives: Development and validation of a single-step and accurate reverse transcriptase loop-mediated isothermal amplification technique (RT-LAMP) for rapid identification of SARS-CoV-2 relative to commercial quantitative reverse transcriptase real ...
M. Jiang, W. Pan, Amir Arasthfer, W. Fang, Liyan Ling, H. Fang, F. Daneshnia, Jian Yu, W. Liao, H. Pei, Xiaojing Li, C. Lass‐Flörl   +11 more
semanticscholar   +1 more source

Inhibition of Non-specific Amplification in Loop-Mediated Isothermal Amplification via Tetramethylammonium Chloride

BioChip Journal, 2022
Loop-mediated isothermal amplification (LAMP) may be used in molecular and point-of-care diagnostics for pathogen detection. The amplification occurs under isothermal conditions using up to six primers.
Minju Jang, Sanghyo Kim
semanticscholar   +1 more source