Results 61 to 70 of about 52,140 (330)

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

PLoS ONE, 2020
A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou, Michael Glenn Mason, Jose Ramon Botella   +2 more
doaj   +1 more source

Accelerated loop-mediated isothermal amplification method for the rapid detection of Streptococcus suis serotypes 2 and 14 based on single nucleotide polymorphisms

Frontiers in Cellular and Infection Microbiology, 2022
Streptococcus suis serotypes 2 and 14 are the most prevalent zoonotic strains. The establishment of a sensitive and extremely accurate method for point-of-care testing for Streptococcus suis serotype 2 and 14 strains is highly desirable. In this study, a
Jiajia Meng, Chunling Li, Chunling Li, Chunling Li, Chunling Li, Yu Wang, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Zhibiao Bian, Pinpin Chu, Pinpin Chu, Pinpin Chu, Pinpin Chu, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Shaolun Zhai, Dongxia Yang, Dongxia Yang, Dongxia Yang, Dongxia Yang, Shuai Song, Shuai Song, Shuai Song, Shuai Song, Yan Li, Yan Li, Yan Li, Yan Li, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Zhiyong Jiang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Kunli Zhang, Yugu Li, Hongchao Gou, Hongchao Gou, Hongchao Gou, Hongchao Gou   +42 more
doaj   +1 more source

A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus [PDF]

The Plant Pathology Journal, 2023
Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, ‘Candidatus Liberibacter asiaticus’ (CLas) is a non-culturable phloem-limited α ...
Tirumalareddy Danda, Jong-Won Park, Kimberly L. Timmons, Mamoudou Sétamou, Eliezer S. Louzada, Madhurababu Kunta   +5 more
doaj   +1 more source

Loop-Mediated Isothermal Amplification for Detection of Plant Pathogens in Wheat (Triticum aestivum)

Frontiers in Plant Science, 2022
Wheat plants can be infected by a variety of pathogen species, with some of them causing similar symptoms. For example, Zymoseptoria tritici and Parastagonospora nodorum often occur together and form the Septoria leaf blotch complex.
Sandra V. Gomez-Gutierrez, S. B. Goodwin
semanticscholar   +1 more source

Molecular Methods for Identification and Quantification of Foodborne Pathogens

Molecules, 2022
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang, Jiajia Wu, Zhaoai Shi, Aocheng Cao, Wensheng Fang, Dongdong Yan, Qiuxia Wang, Yuan Li   +7 more
doaj   +1 more source

Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology

Analytical Chemistry, 2020
We have developed a single-tube assay for SARS-CoV-2 in patient samples. This assay combined advantages of reverse transcription (RT) loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPRs ...
B. Pang, Jingyang Xu, Yanming Liu, Hanyong Peng, Wei Feng, Yiren Cao, Jinjun Wu, Huyan Xiao, K. Pabbaraju, G. Tipples, Michael A. Joyce, H. Saffran, D., Lorne Tyrrell, Hongquan Zhang, X. Le   +15 more
semanticscholar   +1 more source

Detecting amplicons of loop‐mediated isothermal amplification [PDF]

Microbiology and Immunology, 2019
AbstractLoop‐mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real‐time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA.
openaire   +2 more sources

A novel cassette method for probe evaluation in the designed biochips [PDF]

, 2014
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A., Alkhalil, Samia, Asatiani, Nino, Bogdarina, Irina, Kartvelishvili, Tamar, Mitchell, Julian, Sapojnikova, Nelly, Zinkevich, Vitaly   +7 more
core   +4 more sources

Droplet Encoding‐Pairing Enabled Multiplexed Digital Loop‐Mediated Isothermal Amplification for Simultaneous Quantitative Detection of Multiple Pathogens

Advancement of science, 2023
Despite the advantages of digital nucleic acid analysis (DNAA) in terms of sensitivity, precision, and resolution, current DNAA methods commonly suffer a limitation in multiplexing capacity.
Dongyang Cai, Yu Wang, Jingjing Zou, Zhujun Li, Enqi Huang, Xiuyun Ouyang, Zhiquan Que, Yanzhang Luo, Zhenhua Chen, Yanqing Jiang, Guohao Zhang, Hongkai Wu, Dayu Liu   +12 more
semanticscholar   +1 more source

Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACTWhile PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity,
Noritaka, Kuboki, Noboru, Inoue, Tatsuya, Sakurai, Francescopaolo, Di Cello, Dennis J, Grab, Hiroshi, Suzuki, Chihiro, Sugimoto, Ikuo, Igarashi   +7 more
openaire   +2 more sources